Strain identifier

BacDive ID: 13061

Type strain: Yes

Species: Pseudomonas anguilliseptica

Strain Designation: S1

Strain history: CIP <- 2000, CECT <- NCIMB <- S. Egusa: strain S1

NCBI tax ID(s): 53406 (species)

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General

@ref: 4603

BacDive-ID: 13061

DSM-Number: 12111

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile, animal pathogen

description: Pseudomonas anguilliseptica S1 is an aerobe, mesophilic, motile animal pathogen that was isolated from pond-cultured eels .

NCBI tax id

  • NCBI tax id: 53406
  • Matching level: species

strain history

@refhistory
4603<- NCIMB <- S. Egusa
116387CIP <- 2000, CECT <- NCIMB <- S. Egusa: strain S1

doi: 10.13145/bacdive13061.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas anguilliseptica
  • full scientific name: Pseudomonas anguilliseptica Wakabayashi and Egusa 1972 (Approved Lists 1980)

@ref: 4603

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas anguilliseptica

full scientific name: Pseudomonas anguilliseptica Wakabayashi and Egusa 1972

strain designation: S1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.551
6948099.997negative
116387yesnegativerod-shaped

colony morphology

  • @ref: 52773
  • incubation period: 3 days

pigmentation

  • @ref: 116387
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4603CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
39556Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116387CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116387CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
4603positivegrowth25mesophilic
39556positivegrowth25mesophilic
52773positivegrowth30mesophilic
116387positivegrowth10-30
116387nogrowth5psychrophilic
116387nogrowth37mesophilic
116387nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
52773aerobe
116387obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.967

halophily

@refsaltgrowthtested relationconcentration
116387NaClpositivegrowth0-2 %
116387NaClnogrowth4 %
116387NaClnogrowth6 %
116387NaClnogrowth8 %
116387NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
11638716947citrate-carbon source
11638717632nitrate-reduction
11638716301nitrite-reduction

antibiotic resistance

  • @ref: 116387
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11638735581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
116387oxidase+
116387beta-galactosidase-3.2.1.23
116387alcohol dehydrogenase-1.1.1.1
116387gelatinase+/-
116387amylase-
116387DNase-
116387caseinase-3.4.21.50
116387catalase+1.11.1.6
116387tween esterase+
116387lecithinase+
116387lipase-
116387lysine decarboxylase-4.1.1.18
116387ornithine decarboxylase-4.1.1.17
116387protease-
116387tryptophan deaminase-
116387urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116387-++++-----+---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4603-----------------++-+
4603---+-------------+--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116387-------------------------------------------------------------------------------+-----------+-------

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
4603pond-cultured eels (Anguilla japonica)Anguilla japonicaJapanJPNAsia
52773Eels,pond-cultured (Anguilla japonica)
116387Pond-cultured anguilla japonica

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Aquaculture
#Environmental#Aquatic#Pond (small)
#Host#Invertebrates (Other)#Tunicata

taxonmaps

  • @ref: 69479
  • File name: preview.99_8560.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_1186;98_6253;99_8560&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: X99540
  • Sequence Identity:
  • Total samples: 7
  • aquatic counts: 6
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
4603yes1Risk group (German classification)
1163871Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas anguilliseptica 16S-23S intergenic spacer, IGS, strain CECT 899FN646566534ena53406
20218Pseudomonas anguilliseptica partial 16S rRNA gene, strain NCIMB 1949X995401454ena53406
20218Pseudomonas anguilliseptica gene for 16S rRNA, strain: NCIMB 1949AB0213761504ena53406

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas anguilliseptica DSM 12111GCA_900105355contigncbi53406
66792Pseudomonas anguilliseptica strain DSM 1211153406.4wgspatric53406
66792Pseudomonas anguilliseptica DSM 121112687453785draftimg53406

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.81no
anaerobicno98.413yes
halophileno73.853no
spore-formingno95.712no
glucose-utilyes74.035yes
thermophileno99.848yes
flagellatedyes92.555no
aerobicyes93.987yes
motileyes88.786no
glucose-fermentno90.849yes

External links

@ref: 4603

culture collection no.: CCUG 35503, DSM 12111, ATCC 33660, NCIMB 1949, CIP 106711, CECT 899

straininfo link

  • @ref: 82271
  • straininfo: 40607

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30024362Pseudomonas tianjinensis sp. nov., isolated from domestic sewage.Chen P, Li S, Li QXInt J Syst Evol Microbiol10.1099/ijsem.0.0027992018Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny32996879Pseudomonas xionganensis sp. nov., isolated from Baiyangdian Lake in Xiong'an New Area.Zhao Y, Yang Y, He F, Wang C, Feng J, Zhang XInt J Syst Evol Microbiol10.1099/ijsem.0.0044852020Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Lakes/*microbiology, Nucleic Acid Hybridization, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4603Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12111)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12111
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39556Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18872
52773Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35503)https://www.ccug.se/strain?id=35503
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82271Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40607.1StrainInfo: A central database for resolving microbial strain identifiers
116387Curators of the CIPCollection of Institut Pasteur (CIP 106711)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106711