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Strain identifier

BacDive ID: 130555

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: A5803, clone F429

Strain history: <- J. Klockgether, Hannover Medical School, Germany; A5803 <- Univ. Heidelberg, Inst. of Hygiene

NCBI tax ID(s): 287 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 22006

BacDive-ID: 130555

DSM-Number: 29275

keywords: Bacteria, mesophilic, human pathogen

description: Pseudomonas aeruginosa A5803 is a mesophilic human pathogen that was isolated from airways of an intubated patient .

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history: <- J. Klockgether, Hannover Medical School, Germany; A5803 <- Univ. Heidelberg, Inst. of Hygiene

doi: 10.13145/bacdive130555.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 22006

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: A5803, clone F429

type strain: no

Morphology

colony morphology

  • @ref: 22006
  • type of hemolysis: beta
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22006TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
22006LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 22006
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22006catalase+1.11.1.6
22006cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
22006---++++++--++-+++++-+

Isolation, sampling and environmental information

isolation

  • @ref: 22006
  • sample type: airways of an intubated patient (acute infection)
  • geographic location: Heidelberg
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body-Site#Oral cavity and airways#Airways

Safety information

risk assessment

  • @ref: 22006
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa A5803GCA_022870525completencbi287
66792Salmonella enterica subsp. enterica serovar Enteritidis BCW_2195GCA_002030505contigpatric149539

External links

@ref: 22006

culture collection no.: DSM 29275

literature

  • topic: Genetics
  • Pubmed-ID: 34465897
  • title: Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness.
  • authors: Markwitz P, Lood C, Olszak T, van Noort V, Lavigne R, Drulis-Kawa Z
  • journal: ISME J
  • DOI: 10.1038/s41396-021-01096-5
  • year: 2021
  • mesh: Anti-Bacterial Agents, *Bacteriophages/genetics, *Biological Evolution, *Genome, Phenotype, *Prophages/genetics, Pseudomonas aeruginosa/genetics
  • topic2: Phenotype

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22006Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29275Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29275)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE