Strain identifier
BacDive ID: 130555
Type strain: 
Species: Pseudomonas aeruginosa
Strain Designation: A5803, clone F429
Strain history: <- J. Klockgether, Hannover Medical School, Germany; A5803 <- Univ. Heidelberg, Inst. of Hygiene
NCBI tax ID(s): 287 (species)
version 9.3 (current version)
General
@ref: 22006
BacDive-ID: 130555
DSM-Number: 29275
keywords: genome sequence, Bacteria, aerobe, mesophilic, Gram-negative, human pathogen
description: Pseudomonas aeruginosa A5803 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from airways of an intubated patient .
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
- @ref: 22006
- history: <- J. Klockgether, Hannover Medical School, Germany; A5803 <- Univ. Heidelberg, Inst. of Hygiene
doi: 10.13145/bacdive130555.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 22006
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900
strain designation: A5803, clone F429
type strain: no
Morphology
cell morphology
| @ref | gram stain | confidence | motility |
|---|---|---|---|
| 125439 | negative | 95.8 | |
| 125438 | 90.492 | yes | |
| 125438 | negative | 97.5 |
colony morphology
- @ref: 22006
- type of hemolysis: beta
- hemolysis ability: 1
- incubation period: 1-2 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 22006 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| 22006 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | yes | https://mediadive.dsmz.de/medium/381 | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
culture temp
- @ref: 22006
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
oxygen tolerance
- @ref: 125438
- oxygen tolerance: aerobe
- confidence: 95.863
spore formation
- @ref: 125439
- spore formation: no
- confidence: 98.2
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | + | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 22006 | catalase | + | 1.11.1.6 |
| 22006 | cytochrome-c oxidase | + | 1.9.3.1 |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | + | 3.2.1.21 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 22006 | - | - | - | + | + | + | + | + | + | - | - | + | + | - | + | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 22006
- sample type: airways of an intubated patient (acute infection)
- geographic location: Heidelberg
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Disease | |
| #Infection | #Patient | |
| #Host Body-Site | #Oral cavity and airways | #Airways |
Safety information
risk assessment
- @ref: 22006
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
- @ref: 66792
- description: Pseudomonas aeruginosa A5803
- accession: GCA_022870525
- assembly level: complete
- database: ncbi
- NCBI tax ID: 287
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 97.5 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 98.328 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 95.863 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 85.528 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 100 | no |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 90.492 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 98.2 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 87.2 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 95.8 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | aerobe | 64 |
External links
@ref: 22006
culture collection no.: DSM 29275
straininfo link
- @ref: 89075
- straininfo: 399059
literature
- topic: Genetics
- Pubmed-ID: 34465897
- title: Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness.
- authors: Markwitz P, Lood C, Olszak T, van Noort V, Lavigne R, Drulis-Kawa Z
- journal: ISME J
- DOI: 10.1038/s41396-021-01096-5
- year: 2021
- mesh: Anti-Bacterial Agents, *Bacteriophages/genetics, *Biological Evolution, *Genome, Phenotype, *Prophages/genetics, Pseudomonas aeruginosa/genetics
- topic2: Phenotype
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22006 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29275 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29275) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68369 | Automatically annotated from API 20NE | |||
| 89075 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399059.1 | |
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | 10.1101/2024.08.12.607695 | |
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 | https://github.com/GenomeNet/deepG |