Strain identifier
BacDive ID: 130415
Type strain: 
Species: Microbacterium proteolyticum
Strain Designation: RZ36
Strain history: <- A. Alves, Univ. de Aveiro, Dep. de Biologie, Portugal; RZ36
NCBI tax ID(s): 1572644 (species)
version 8.1 (current version)
General
@ref: 21668
BacDive-ID: 130415
DSM-Number: 27100
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive
description: Microbacterium proteolyticum RZ36 is a mesophilic, Gram-positive bacterium that was isolated from surface sterilized roots of sea purslane .
NCBI tax id
- NCBI tax id: 1572644
- Matching level: species
strain history
- @ref: 21668
- history: <- A. Alves, Univ. de Aveiro, Dep. de Biologie, Portugal; RZ36
doi: 10.13145/bacdive130415.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Microbacteriaceae
- genus: Microbacterium
- species: Microbacterium proteolyticum
- full scientific name: Microbacterium proteolyticum Alves et al. 2015
@ref: 21668
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Microbacteriaceae
genus: Microbacterium
species: Microbacterium proteolyticum
full scientific name: Microbacterium proteolyticum Alves et al. 2015
strain designation: RZ36
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
- @ref: 21668
- name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
- growth: yes
- link: https://mediadive.dsmz.de/medium/92
- composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
culture temp
- @ref: 21668
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.959 |
Isolation, sampling and environmental information
isolation
- @ref: 21668
- sample type: surface sterilized roots of sea purslane (Halimione portulacoides)
- host species: Halimione portulacoides
- geographic location: Ria de Aveiro, Largo do Laranjo, Murtosa (40° 43' 48.0'' N 8° 36' 45.5'' W)
- country: Portugal
- origin.country: PRT
- continent: Europe
- latitude: 40.73
- longitude: -8.6126
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Shrub (Scrub) |
| #Host Body-Site | #Plant | #Root (Rhizome) |
| #Host Body-Site | #Plant | #Sterilized plant part |
taxonmaps
- @ref: 69479
- File name: preview.99_322.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_137;97_246;98_275;99_322&stattab=map
- Last taxonomy: Microbacterium
- 16S sequence: KM359785
- Sequence Identity:
- Total samples: 286
- soil counts: 34
- aquatic counts: 27
- animal counts: 89
- plant counts: 136
Safety information
risk assessment
- @ref: 21668
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21668
- description: Microbacterium proteolyticum strain RZ36 16S ribosomal RNA gene, partial sequence
- accession: KM359785
- length: 1436
- database: ena
- NCBI tax ID: 1572644
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Microbacterium proteolyticum CECT 8356 | GCA_014192415 | scaffold | ncbi | 1572644 |
| 66792 | Microbacterium proteolyticum strain CECT 8356 | 1572644.3 | wgs | patric | 1572644 |
| 66792 | Microbacterium proteolyticum CECT 8356 | 2824465740 | draft | img | 1572644 |
GC content
- @ref: 21668
- GC-content: 69.7
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| flagellated | no | 97.696 | no |
| gram-positive | yes | 94.218 | no |
| anaerobic | no | 99.722 | no |
| aerobic | yes | 95.905 | no |
| halophile | no | 87.351 | no |
| spore-forming | no | 96.24 | no |
| glucose-util | yes | 86.991 | no |
| thermophile | no | 99.097 | no |
| motile | no | 92.481 | no |
| glucose-ferment | no | 92.084 | no |
External links
@ref: 21668
culture collection no.: DSM 27100, CECT 8356
straininfo link
- @ref: 88947
- straininfo: 399720
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 25744585 | Microbacterium proteolyticum sp. nov. isolated from roots of Halimione portulacoides. | Alves A, Riesco R, Correia A, Trujillo ME | Int J Syst Evol Microbiol | 10.1099/ijs.0.000177 | 2015 | Actinomycetales/*classification/genetics/isolation & purification, Amaranthaceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, Plant Roots/*microbiology, Portugal, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry | Genetics |
| Phylogeny | 28176143 | Microbacterium zeae sp. nov., an endophytic bacterium isolated from maize stem. | Gao JL, Sun P, Wang XM, Lv FY, Sun JG | Antonie Van Leeuwenhoek | 10.1007/s10482-017-0837-3 | 2017 | Actinobacteria/*classification/genetics/*isolation & purification, Aerobiosis, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Endophytes/*classification/genetics/*isolation & purification, Fatty Acids/analysis, Glycolipids/analysis, Humans, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Plant Stems/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysis, Zea mays/*microbiology | Enzymology |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21668 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27100 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27100) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 88947 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399720.1 |