Strain identifier

BacDive ID: 12908

Type strain: No

Species: Pseudomonas putida

Strain Designation: Strain A

Strain history: CIP <- 2002, NCIMB <- BKM

NCBI tax ID(s): 303 (species)

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General

@ref: 3116

BacDive-ID: 12908

DSM-Number: 7314

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Pseudomonas putida Strain A is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from Water samples taken from areas of mass development of S. eusphaera.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

@refhistory
3116<- NCIMB; NCIMB 11286 <- VKM; VKM-B 1122 <- G. Dubinina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Strain A
67770IAM 14870 <-- NCIMB 11286 <-- BKM BN-1122.
122068CIP <- 2002, NCIMB <- BKM

doi: 10.13145/bacdive12908.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 3116

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: Strain A

type strain: no

Morphology

cell morphology

  • @ref: 122068
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 122068
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3116NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605)yeshttps://mediadive.dsmz.de/medium/605Name: NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605) Composition: Agar 15.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Lab-Lemco beef extract 1.0 g/l Distilled water
32680MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122068CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
3116positivegrowth25mesophilic
32680positivegrowth25mesophilic
67770positivegrowth25mesophilic
122068positivegrowth5-30
122068nogrowth37mesophilic
122068nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122068
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122068NaClpositivegrowth0-6 %
122068NaClnogrowth8 %
122068NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12206816947citrate+carbon source
1220684853esculin-hydrolysis
12206817632nitrate-reduction
12206816301nitrite-reduction
12206817632nitrate-respiration

antibiotic resistance

  • @ref: 122068
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 122068
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
122068oxidase+
122068beta-galactosidase-3.2.1.23
122068alcohol dehydrogenase+1.1.1.1
122068gelatinase-
122068amylase-
122068DNase-
122068caseinase-3.4.21.50
122068catalase+1.11.1.6
122068tween esterase-
122068lecithinase-
122068lipase-
122068lysine decarboxylase-4.1.1.18
122068ornithine decarboxylase-4.1.1.17
122068protease-
122068tryptophan deaminase-
122068urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122068--++-+--+-++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122068++-----------------+-----------++----------+++-++++--++++---++++++-+--++++++++-++++++-+-++++++++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3116Water samples taken from areas of mass development of S. eusphaeraGek-GelAzerbaijanAZEAsia
122068Gek-GelAzerbaijanAZEAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_863.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_863&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF226713
  • Sequence Identity:
  • Total samples: 1972
  • soil counts: 444
  • aquatic counts: 421
  • animal counts: 802
  • plant counts: 305

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
3116yesyes2Risk group (German classification)
1220681Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arthrobacter siderocapsulatus (strain NCIMB 11286) 16S rRNA gene, partialAJ0079101437ena303
3116Arthrobacter siderocapsulatus 16S ribosomal RNA gene, complete sequenceAF2267131442ena303

External links

@ref: 3116

culture collection no.: DSM 7314, NCIMB 11286, VKM B-1122, JCM 21368, CIP 107361, IAM 14870, IMET 11317, KCTC 9839, LMG 16206, BKM BN1122

straininfo link

  • @ref: 82121
  • straininfo: 8187

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11211254Arthrobacter siderocapsulatus Dubinina and Zhdanov 1975AL is a later subjective synonym of Pseudomonas putida (Trevisan 1889) Migula 1895AL.Chun J, Rhee MS, Han JI, Bae KSInt J Syst Evol Microbiol10.1099/00207713-51-1-1692001Arthrobacter/chemistry/*classification/genetics, Bacterial Typing Techniques/methods, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Pseudomonas putida/chemistry/*classification/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Biotechnology36367470Engineering artificial microbial consortia based on division of labor promoted simultaneous removal of Cr(VI)-atrazine combined pollution.Wu S, Li X, Fan H, Dong Y, Wang Y, Bai Z, Zhuang XJ Hazard Mater10.1016/j.jhazmat.2022.1302212022*Microbial Consortia, *Atrazine/analysis, Chromium/metabolism, Biodegradation, EnvironmentalMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3116Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7314)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7314
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32680Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4788
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82121Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8187.1StrainInfo: A central database for resolving microbial strain identifiers
122068Curators of the CIPCollection of Institut Pasteur (CIP 107361)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107361