Strain identifier

BacDive ID: 12850

Type strain: No

Species: Pseudomonas fluorescens

Strain Designation: SK1

Strain history: <- W. Reineke, Uni Wuppertal; SK1 <- S. R. Kaschabek, TU Bergakademie Freiberg

NCBI tax ID(s): 294 (species)

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General

@ref: 6268

BacDive-ID: 12850

DSM-Number: 16274

keywords: Bacteria, mesophilic, human pathogen

description: Pseudomonas fluorescens SK1 is a mesophilic human pathogen that was isolated from ground water.

NCBI tax id

  • NCBI tax id: 294
  • Matching level: species

strain history

  • @ref: 6268
  • history: <- W. Reineke, Uni Wuppertal; SK1 <- S. R. Kaschabek, TU Bergakademie Freiberg

doi: 10.13145/bacdive12850.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas fluorescens
  • full scientific name: Pseudomonas fluorescens Migula 1895 (Approved Lists 1980)

@ref: 6268

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas fluorescens

full scientific name: Pseudomonas fluorescens Migula 1895

strain designation: SK1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6268REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
6268MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://mediadive.dsmz.de/medium/457Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water

culture temp

  • @ref: 6268
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

compound production

  • @ref: 6268
  • compound: chlorocatechol 2,3-dioxygenase CbzE

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6268+--+----+++++-+++++-+

Isolation, sampling and environmental information

isolation

  • @ref: 6268
  • sample type: ground water
  • geographic location: Bitterfeld
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Groundwater

Safety information

risk assessment

  • @ref: 6268
  • pathogenicity human: yes, in single cases
  • pathogenicity animal: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 6268

culture collection no.: DSM 16274

straininfo link

  • @ref: 82062
  • straininfo: 404883

literature

  • topic: Enzymology
  • Pubmed-ID: 15340793
  • title: Microorganisms degrading chlorobenzene via a meta-cleavage pathway harbor highly similar chlorocatechol 2,3-dioxygenase-encoding gene clusters.
  • authors: Gobel M, Kranz OH, Kaschabek SR, Schmidt E, Pieper DH, Reineke W
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-004-0681-5
  • year: 2004
  • mesh: Aldehyde Oxidoreductases/genetics, Amino Acid Sequence, Bacterial Proteins/genetics/metabolism, Biodegradation, Environmental, Catechol 2,3-Dioxygenase, Catechols/metabolism, Chlorobenzenes/*metabolism, Cloning, Molecular, DNA, Bacterial/chemistry/isolation & purification, Dioxygenases/chemistry/*genetics/isolation & purification/*metabolism, Environmental Microbiology, Ferredoxins/genetics, Genes, Bacterial, Molecular Sequence Data, Operon, Phylogeny, Pseudomonas/*enzymology/*genetics/isolation & purification/metabolism, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Synteny
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
6268Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16274)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16274
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
82062Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404883.1StrainInfo: A central database for resolving microbial strain identifiers