Strain identifier
BacDive ID: 12848
Type strain: 
Species: Pseudomonas fluorescens
Strain Designation: Tween B
Strain history: <- E. Lang; TweenB
NCBI tax ID(s): 294 (species)
version 8.1 (current version)
General
@ref: 3766
BacDive-ID: 12848
DSM-Number: 9958
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas fluorescens Tween B is a mesophilic human pathogen that was isolated from contaminant of PBS-Tween solution.
NCBI tax id
- NCBI tax id: 294
- Matching level: species
strain history
- @ref: 3766
- history: <- E. Lang; TweenB
doi: 10.13145/bacdive12848.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas fluorescens
- full scientific name: Pseudomonas fluorescens Migula 1895 (Approved Lists 1980)
@ref: 3766
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas fluorescens
full scientific name: Pseudomonas fluorescens Migula 1895
strain designation: Tween B
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 3766 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
| 3766 | MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) | yes | https://mediadive.dsmz.de/medium/457 | Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457; with strain-specific modifications) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Tween 20 Distilled water |
culture temp
- @ref: 3766
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3766 | + | - | - | + | - | - | + | - | + | + | + | + | + | - | + | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 3766
- sample type: contaminant of PBS-Tween solution
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
- Cat1: #Engineered
- Cat2: #Laboratory
- Cat3: #Contaminant
Safety information
risk assessment
- @ref: 3766
- pathogenicity human: yes, in single cases
- pathogenicity animal: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 3766
culture collection no.: DSM 9958
straininfo link
- @ref: 82060
- straininfo: 48724
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3766 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9958) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9958 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82060 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48724.1 | StrainInfo: A central database for resolving microbial strain identifiers |