Strain identifier
BacDive ID: 12846
Type strain: 
Species: Pseudomonas fluorescens
Strain Designation: 4
Strain history: <- G. Berg, 4
NCBI tax ID(s): 294 (species)
version 9 (current version)
General
@ref: 3300
BacDive-ID: 12846
DSM-Number: 8567
keywords: Bacteria, human pathogen
description: Pseudomonas fluorescens 4 is a human pathogen that was isolated from rhizosphere of Brassica napus.
NCBI tax id
- NCBI tax id: 294
- Matching level: species
strain history
- @ref: 3300
- history: <- G. Berg, 4
doi: 10.13145/bacdive12846.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas fluorescens
- full scientific name: Pseudomonas fluorescens Migula 1895 (Approved Lists 1980)
@ref: 3300
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas fluorescens
full scientific name: Pseudomonas fluorescens Migula 1895
strain designation: 4
type strain: no
Culture and growth conditions
culture medium
- @ref: 3300
- name: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220a
- composition: Name: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
culture temp
- @ref: 3300
- growth: positive
- type: growth
- temperature: 28
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3300 | + | - | - | - | - | - | + | - | + | + | + | + | + | - | + | + | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 3300
- sample type: rhizosphere of Brassica napus
- host species: Brassica napus
- geographic location: Rostock
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Rhizosphere |
Safety information
risk assessment
- @ref: 3300
- pathogenicity human: yes, in single cases
- pathogenicity animal: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 3300
culture collection no.: DSM 8567
straininfo link
- @ref: 82058
- straininfo: 48495
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3300 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8567) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8567 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82058 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48495.1 | StrainInfo: A central database for resolving microbial strain identifiers |