Strain identifier
BacDive ID: 12795
Type strain: 
Species: Pseudomonas aeruginosa
Strain Designation: PAO1
Strain history: <- M. Schobert, Inst. Microbiology, TU Braunschweig, Germany; PAO1 <- S. Lory, Univ. of Washington, Seattle, USA <- ? <- <- P. Rountree
NCBI tax ID(s): 287 (species)
version 8.1 (current version)
General
@ref: 8364
BacDive-ID: 12795
DSM-Number: 19880
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas aeruginosa PAO1 is a mesophilic human pathogen that was isolated from human, infected wound.
NCBI tax id
- NCBI tax id: 287
- Matching level: species
strain history
- @ref: 8364
- history: <- M. Schobert, Inst. Microbiology, TU Braunschweig, Germany; PAO1 <- S. Lory, Univ. of Washington, Seattle, USA <- ? <- <- P. Rountree
doi: 10.13145/bacdive12795.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas aeruginosa
- full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacterium aeruginosum
@ref: 8364
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas aeruginosa
full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900
strain designation: PAO1
type strain: no
Culture and growth conditions
culture medium
- @ref: 8364
- name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
- growth: yes
- link: https://mediadive.dsmz.de/medium/381
- composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
culture temp
- @ref: 8364
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8364 | - | - | - | + | + | - | + | - | + | - | - | + | + | - | + | + | + | + | + | - | + |
| 8364 | - | - | - | + | + | - | + | - | + | - | - | + | + | - | + | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 8364
- sample type: human, infected wound
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Disease | |
| #Host | #Human | |
| #Host Body-Site | #Other | #Wound |
Safety information
risk assessment
- @ref: 8364
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 8364
culture collection no.: DSM 19880
straininfo link
- @ref: 82005
- straininfo: 397215
literature
- topic: Pathogenicity
- Pubmed-ID: 34989245
- title: Characterization and in vitro testing of newly isolated lytic bacteriophages for the biocontrol of Pseudomonas aeruginosa.
- authors: Harada LK, Silva EC, Rossi FP, Cieza B, Oliveira TJ, Pereira C, Tomazetto G, Silva BB, Squina FM, Vila MM, Setubal JC, Ha T, da Silva AM, Balcao VM
- journal: Future Microbiol
- DOI: 10.2217/fmb-2021-0027
- year: 2022
- mesh: *Bacteriophages/genetics, Host Specificity, In Vitro Techniques, Myoviridae/genetics, *Pseudomonas aeruginosa
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 8364 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19880) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19880 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82005 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397215.1 | StrainInfo: A central database for resolving microbial strain identifiers |