Strain identifier

BacDive ID: 12703

Type strain: No

Species: Azorhizophilus paspali

Strain Designation: Ax-12

Strain history: <- J. Döbereiner, Ax-12

NCBI tax ID(s): 69963 (species)

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General

@ref: 164

BacDive-ID: 12703

DSM-Number: 376

keywords: Bacteria, mesophilic

description: Azorhizophilus paspali Ax-12 is a mesophilic bacterium that was isolated from rhizosphere soil of Paspalum notatum.

NCBI tax id

  • NCBI tax id: 69963
  • Matching level: species

strain history

  • @ref: 164
  • history: <- J. Döbereiner, Ax-12

doi: 10.13145/bacdive12703.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Azorhizophilus
  • species: Azorhizophilus paspali
  • full scientific name: Azorhizophilus paspali (Döbereiner 1966) Thompson and Skerman 1981
  • synonyms

    • @ref: 20215
    • synonym: Azotobacter paspali

@ref: 164

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Azorhizophilus

species: Azorhizophilus paspali

full scientific name: Azorhizophilus paspali (Döbereiner 1966) Thompson and Skerman 1981

strain designation: Ax-12

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
164AZOTOBACTER MEDIUM (DSMZ Medium 3)yeshttps://mediadive.dsmz.de/medium/3Name: AZOTOBACTER MEDIUM (DSMZ Medium 3) Composition: Agar 15.0 g/l CaCO3 5.0 g/l Glucose 5.0 g/l Mannitol 5.0 g/l K2HPO4 0.9 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.1 g/l FeSO4 x 7 H2O 0.01 g/l Na2MoO4 x 2 H2O 0.005 g/l Distilled water
164DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441)yeshttps://mediadive.dsmz.de/medium/441Name: DIAZOTROPHIC MEDIUM (RBA) (DSMZ Medium 441) Composition: Agar 14.881 g/l DL-Malate 1.98413 g/l D-Glucose 1.98413 g/l D-Mannitol 1.98413 g/l Na-pyruvate 0.992063 g/l Disodium succinate 0.992063 g/l K2HPO4 0.892857 g/l KH2PO4 0.0992065 g/l NaCl 0.0992065 g/l CaCl2 x 2 H2O 0.0992065 g/l MgSO4 x 7 H2O 0.0992065 g/l Yeast extract 0.0496032 g/l FeSO4 x 7 H2O 0.00992065 g/l MnSO4 x H2O 0.00496032 g/l NaVO3 x H2O 0.00496032 g/l Nicotinic acid 0.00248016 g/l Pyridoxine hydrochloride 0.00248016 g/l Calcium pantothenate 0.00248016 g/l Thiamine-HCl x 2 H2O 0.00248016 g/l H3BO3 0.000892857 g/l CoCl2 x 6 H2O 0.000595238 g/l Riboflavin 0.000496032 g/l ZnSO4 x 7 H2O 0.000297619 g/l MnCl2 x 4 H2O 8.92857e-05 g/l Na2MoO4 x 2 H2O 8.92857e-05 g/l NiCl2 x 6 H2O 5.95238e-05 g/l Vitamin B12 4.96032e-05 g/l CuCl2 x 2 H2O 2.97619e-05 g/l Folic acid 9.92063e-06 g/l Biotin 4.96032e-06 g/l Distilled water

culture temp

  • @ref: 164
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 164
  • sample type: rhizosphere soil of Paspalum notatum
  • host species: Paspalum notatum
  • country: Brazil
  • origin.country: BRA
  • continent: Middle and South America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 164
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 164
  • GC-content: 63.8

External links

@ref: 164

culture collection no.: DSM 376, ATCC 23367

straininfo link

  • @ref: 81913
  • straininfo: 1030

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1987127Detection of alternative nitrogenases in aerobic gram-negative nitrogen-fixing bacteria.Fallik E, Chan YK, Robson RLJ Bacteriol10.1128/jb.173.1.365-371.19911991Azotobacter/enzymology/genetics, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Escherichia coli/genetics, *Genes, Bacterial, Gram-Negative Aerobic Bacteria/enzymology/*genetics, *Nitrogen Fixation, Nitrogenase/*genetics, Nucleic Acid Hybridization, Pseudomonas/enzymology/genetics, Rhizobium/enzymology/genetics, Species SpecificityPhylogeny
16348965Presence of a Vanadium Nitrogenase in Azotobacter paspali.Fallik E, Hartel PG, Robson RLAppl Environ Microbiol10.1128/aem.59.6.1883-1886.19931993

Reference

@idauthorscataloguedoi/urltitle
164Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 376)https://www.dsmz.de/collection/catalogue/details/culture/DSM-376
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
81913Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1030.1StrainInfo: A central database for resolving microbial strain identifiers