Strain identifier

BacDive ID: 12654

Type strain: Yes

Species: Acidipropionibacterium thoenii

Strain Designation: 15

Strain history: CIP <- 1987, NCIB <- 1950, NCTC <- 1940, ATCC <- C.B. van Niel: strain 15

NCBI tax ID(s): 1122998 (strain), 1751 (species)

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General

@ref: 8683

BacDive-ID: 12654

DSM-Number: 20276

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Acidipropionibacterium thoenii 15 is an anaerobe, mesophilic bacterium that was isolated from emmental cheese.

NCBI tax id

NCBI tax idMatching level
1122998strain
1751species

strain history

@refhistory
8683<- Weihenstephan, Germany <- ATCC <- C.B. van Niel, 15
67770T. Mitsuoka <-- ATCC 4874 <-- C. B. van Niel 15.
123531CIP <- 1987, NCIB <- 1950, NCTC <- 1940, ATCC <- C.B. van Niel: strain 15

doi: 10.13145/bacdive12654.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Acidipropionibacterium
  • species: Acidipropionibacterium thoenii
  • full scientific name: Acidipropionibacterium thoenii (van Niel 1928) Scholz and Kilian 2016

  • synonyms

    • @ref: 20215
    • synonym: Propionibacterium thoenii

@ref: 8683

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Acidipropionibacterium

species: Acidipropionibacterium thoenii

full scientific name: Acidipropionibacterium thoenii (van Niel 1928) Scholz and Kilian 2016 emend. Nouioui et al. 2018

strain designation: 15

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.897
69480100positive
123531nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8683PROPIONIBACTERIUM AGAR (DSMZ Medium 91)yeshttps://mediadive.dsmz.de/medium/91Name: PROPIONIBACTERIUM AGAR (DSMZ Medium 91) Composition: Agar 15.0 g/l Na-lactate 10.0 g/l Casein peptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
42203MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
123531CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20
123531CIP Medium 26yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=26

culture temp

@refgrowthtypetemperaturerange
8683positivegrowth30mesophilic
42203positivegrowth30mesophilic
49122positivegrowth37mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8683anaerobe
49122anaerobe
49122microaerophile
123531anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.997

murein

  • @ref: 8683
  • murein short key: A41.01
  • type: A3gamma LL-Dpm-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12353117632nitrate-reduction
12353116301nitrite-reduction
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
12353135581indoleno
6838035581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin-
6838035581indole-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380tryptophan deaminase-4.1.99.1
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
123531catalase+1.11.1.6
123531urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49122C15:09.615
    49122C16:04.416
    49122C17:0317
    49122C18:02.718
    49122C14:0 3OH/C16:1 ISO I2.715.485
    49122C14:0 ISO 3OH1.715.117
    49122C15:0 ANTEISO16.214.711
    49122C15:0 ISO2314.621
    49122C16:0 iso1.115.626
    49122C16:1 ω11c0.915.757
    49122C17:0 anteiso1.616.722
    49122C17:0 iso3.616.629
    49122C17:1 ISO I/C16:0 DMA116.481
    49122C18:1 ω7c /12t/9t18.717.824
    49122C18:1 ω9c3.217.769
    49122C18:2 ω6,9c/C18:0 ANTE4.517.724
    49122Unidentified2.2
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
49122---+-++++---++--+-+--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
49122-+---+++--++-+-++++-

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8683---+-++---+------++-++-+-+/-+/--+
49122---+-++---+--+--++++++-++++-+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
49122------------------+-------------

Isolation, sampling and environmental information

isolation

@refsample type
8683emmental cheese
49122Emmental cheese
67770Emmenthaler cheese
123531Food, Cheese, Emmenthal

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86831Risk group (German classification)
1235311Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Propionibacterium thoenii 16S-23S ribosomal RNA intergenic spacer region, complete sequenceAF218435268ena1751
20218Propionibacterium thoenii strain CIP 103029 16S ribosomal RNA gene, partial sequenceAY883045461ena1751
20218Propionibacterium thoenii partial 16S rRNAX532201467ena1751
20218Propionibacterium thoenii 16S rRNA (partial) strain NCFB 568AJ132324465ena1751
20218Propionibacterium thoenii partial 16S rRNA gene, type strain NCFB 568AJ7045721467ena1751

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Propionibacterium thoenii DSM 202761122998.3wgspatric1122998
66792Acidipropionibacterium thoenii DSM 202762523231069draftimg1122998
67770Acidipropionibacterium thoenii DSM 20276GCA_000423445scaffoldncbi1122998

GC content

@refGC-content
868366.0
6777066

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno85no
motileno94.764no
flagellatedno98.424no
gram-positiveyes91.257no
anaerobicno65.235no
aerobicno83.012yes
halophileno92.969no
spore-formingno93.111no
thermophileno87.688yes
glucose-utilyes89.5no
glucose-fermentyes67.815yes

External links

@ref: 8683

culture collection no.: DSM 20276, ATCC 4874, CCM 1865, CCUG 28149, HAMBI 247, JCM 6437, LMG 16455, NCDO 568, NCIB 5966, CGMCC 1.2228, CIP 103029, LMG 16731, NCIMB 5966, VPI 0412, VTT E-79095

straininfo link

  • @ref: 81867
  • straininfo: 8650

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8683Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20276)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20276
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42203Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14781
49122Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28149)https://www.ccug.se/strain?id=28149
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
81867Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8650.1StrainInfo: A central database for resolving microbial strain identifiers
123531Curators of the CIPCollection of Institut Pasteur (CIP 103029)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103029