Strain identifier

BacDive ID: 1264

Type strain: Yes

Species: Bacillus safensis

Strain Designation: FO-36b

Strain history: CIP <- 2006, K. Venkateswaran, Lund Univ., Pasadena, California, USA: strain FO-36b

NCBI tax ID(s): 561879 (species)

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General

@ref: 8001

BacDive-ID: 1264

DSM-Number: 19292

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, chemoheterotroph, spore-forming, thermophilic, colony-forming

description: Bacillus safensis FO-36b is a facultative anaerobe, chemoheterotroph, spore-forming bacterium that forms round, undulate colonies and was isolated from clean room air particle, spacecraft assembly facility.

NCBI tax id

  • NCBI tax id: 561879
  • Matching level: species

strain history

@refhistory
8001<- NBRC <- K. Venkateswaran, FO-36b
122346CIP <- 2006, K. Venkateswaran, Lund Univ., Pasadena, California, USA: strain FO-36b

doi: 10.13145/bacdive1264.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus safensis
  • full scientific name: Bacillus safensis Satomi et al. 2006

@ref: 8001

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus safensis subsp. safensis

full scientific name: Bacillus safensis subsp. safensis (Satomi et al. 2006) Abril et al. 2019

strain designation: FO-36b

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthgram staincell shapemotility
229021-1.2 µm0.5-0.7 µm
122346positiverod-shapedyes

colony morphology

  • @ref: 22902
  • colony color: white
  • colony shape: round, undulate

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8001CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
33297MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122346CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
22902positivemaximum50thermophilic
22902positiveminimum10psychrophilic
22902positivegrowth30-37mesophilic
8001positivegrowth30mesophilic
33297positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122346
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 22902
  • type: chemoheterotroph

spore formation

  • @ref: 22902
  • spore formation: yes

compound production

  • @ref: 22902
  • compound: Acetoin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2290117057cellobiose+builds acid from
2290117634D-glucose+builds acid from
2290116024D-mannose-builds acid from
2290117113erythritol+builds acid from
2290117268myo-inositol-builds acid from
2290116467L-arginine+growth
2290117306maltose-builds acid from
2290129864mannitol+builds acid from
2290116634raffinose-builds acid from
2290126546rhamnose-builds acid from
2290130911sorbitol-builds acid from
2290128017starch-builds acid from
22902168082-dehydro-D-gluconate-builds acid from
22902168082-dehydro-D-gluconate-growth
22902581435-dehydro-D-gluconate-builds acid from
22902581435-dehydro-D-gluconate-growth
2290217128adipate-growth
2290215963ribitol-builds acid from
2290215963ribitol-growth
2290227613amygdalin+builds acid from
2290227613amygdalin+growth
2290218305arbutin+builds acid from
2290218305arbutin+growth
2290227689decanoate-growth
22902casein-hydrolysis
2290217057cellobiose+builds acid from
2290217057cellobiose+growth
2290216947citrate+growth
2290217108D-arabinose-builds acid from
2290217108D-arabinose-growth
2290218333D-arabitol-builds acid from
2290218333D-arabitol-growth
2290228847D-fucose-builds acid from
2290228847D-fucose-growth
2290217634D-glucose+builds acid from
2290217634D-glucose+growth
2290217634D-glucose-builds gas from
2290262318D-lyxose-builds acid from
2290262318D-lyxose-growth
2290216443D-tagatose+builds acid from
2290216443D-tagatose+growth
2290232528turanose+builds acid from
2290232528turanose+growth
2290265327D-xylose+builds acid from
2290265327D-xylose+growth
2290216813galactitol-builds acid from
2290216813galactitol-growth
2290217113erythritol-builds acid from
2290217113erythritol-growth
229024853esculin+hydrolysis
2290228757fructose+builds acid from
2290228757fructose+growth
2290228260galactose+builds acid from
2290228260galactose+growth
229025291gelatin+hydrolysis
2290228066gentiobiose+/-builds acid from
2290228066gentiobiose+growth
2290224265gluconate-builds acid from
2290224265gluconate+growth
2290217754glycerol+builds acid from
2290217754glycerol+growth
2290215428glycine-growth
2290228087glycogen-builds acid from
2290228087glycogen-growth
2290217268myo-inositol+builds acid from
2290217268myo-inositol+growth
2290215443inulin-builds acid from
2290215443inulin-growth
2290230849L-arabinose+builds acid from
2290230849L-arabinose+growth
2290218403L-arabitol-builds acid from
2290218403L-arabitol-growth
2290229991L-aspartate+growth
2290218287L-fucose-builds acid from
2290218287L-fucose-growth
2290229985L-glutamate+growth
2290265328L-xylose-builds acid from
2290265328L-xylose-growth
2290224996lactate+growth
2290217716lactose+/-builds acid from
2290217716lactose-growth
2290225115malate+growth
2290217306maltose+builds acid from
2290217306maltose+growth
2290229864mannitol+builds acid from
2290229864mannitol+growth
2290237684mannose+builds acid from
2290237684mannose+growth
229026731melezitose-builds acid from
229026731melezitose-growth
2290228053melibiose+/-builds acid from
2290228053melibiose+growth
22902320061methyl alpha-D-glucopyranoside+builds acid from
22902320061methyl alpha-D-glucopyranoside+growth
2290243943methyl alpha-D-mannoside+builds acid from
2290243943methyl alpha-D-mannoside+growth
2290274863methyl beta-D-xylopyranoside-builds acid from
2290274863methyl beta-D-xylopyranoside-growth
22902506227N-acetyl-D-glucosamine+builds acid from
22902506227N-acetyl-D-glucosamine+growth
2290218401phenylacetate-growth
2290217272propionate-growth
2290216634raffinose-builds acid from
2290216634raffinose+growth
2290226546rhamnose-builds acid from
2290226546rhamnose+/-growth
2290233942ribose+builds acid from
2290233942ribose+growth
2290233697rna+hydrolysis
2290217814salicin+builds acid from
2290217814salicin+growth
2290230911sorbitol-builds acid from
2290230911sorbitol-growth
2290227922sorbose-builds acid from
2290227922sorbose-growth
2290228017starch-builds acid from
2290228017starch-growth
2290217992sucrose+builds acid from
2290217992sucrose+growth
2290227082trehalose+builds acid from
2290227082trehalose+growth
2290217151xylitol-builds acid from
2290217151xylitol-growth
2290217632nitrate-reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
12234617632nitrate-reduction
12234616301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2290216136hydrogen sulfideno
2290235581indoleno
2290215688acetoinyes
12234635581indoleno

metabolite tests

  • @ref: 22902
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
22901lipase (Tween 80)-
22902alkaline phosphatase+3.1.3.1
22902alpha-fucosidase-3.2.1.51
22902alpha-galactosidase-3.2.1.22
22902arginine dihydrolase-3.5.3.6
22902beta-galactosidase+3.2.1.23
22902beta-glucosidase+3.2.1.21
22902catalase+1.11.1.6
22902cystine arylamidase-3.4.11.3
22902gelatinase+
22902leucine arylamidase-3.4.11.1
22902lysine decarboxylase-4.1.1.18
22902N-acetyl-beta-glucosaminidase-3.2.1.52
22902ornithine decarboxylase-4.1.1.17
22902cytochrome oxidase+1.9.3.1
22902trypsin-3.4.21.4
22902tryptophan deaminase-4.1.99.1
22902valine arylamidase-
22902lipase (Tween 80)-
22902amylase-
22902esterase+
22902agarase-3.2.1.81
22902lecithinase-
22902DNase-
22902urease-3.5.1.5
22902phenylalanine deaminase-4.3.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122346oxidase+
122346catalase+1.11.1.6
122346urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122346-+++-----++--+--+-+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122346+/---+++/----+/-+++---+/-+-+/-+/-+++++++/---++------++/--+-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8001clean room air particle, spacecraft assembly facilityPasadenaUSAUSANorth America
122346Spacecraft assembly facilityPasadena, CaliforniaUnited States of AmericaUSANorth America2001

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Clean room
#Environmental#Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_885.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_607;98_698;99_885&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: AB681259
  • Sequence Identity:
  • Total samples: 20329
  • soil counts: 7187
  • aquatic counts: 3497
  • animal counts: 6743
  • plant counts: 2902

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
80011Risk group (German classification)
1223461Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus safensis gene for 16S rRNA, partial sequence, strain: NBRC 100820AB6812591474ena561879
8001Bacillus safensis strain FO-036b 16S ribosomal RNA gene, partial sequenceAF2348541434ena1178541

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus safensis FO-36bGCA_003097715chromosomencbi1178541
66792Bacillus safensis FO-36bGCA_000691165contigncbi1178541
66792Bacillus safensis FO-36b1178541.10wgspatric1178541
66792Bacillus safensis FO-36b1178541.5wgspatric1178541
66792Bacillus safensis FO-36b2576861488draftimg1178541

GC content

  • @ref: 8001
  • GC-content: 41

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes87.076no
gram-positiveyes86.658no
anaerobicno99.258no
halophileyes77.713no
spore-formingyes96.344yes
glucose-utilyes88.762yes
aerobicyes88.281no
thermophileno95.474no
motileyes89.751no
glucose-fermentno89.405no

External links

@ref: 8001

culture collection no.: DSM 19292, ATCC BAA 1126, LMG 26769, NBRC 100820, CIP 109412

straininfo link

  • @ref: 70920
  • straininfo: 297138

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16902000Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces.Satomi M, La Duc MT, Venkateswaran KInt J Syst Evol Microbiol10.1099/ijs.0.64189-02006Bacillus/*classification/genetics/isolation & purification, Bacterial Proteins/genetics, DNA Fingerprinting, DNA Gyrase/genetics, DNA, Bacterial/genetics, *Environmental Microbiology, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Repetitive Sequences, Nucleic Acid/genetics, Sequence Homology, Nucleic Acid, *Spacecraft, Species SpecificityEnzymology
Phylogeny24158533Bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (Mugil cephalus).Lai Q, Liu Y, Shao ZAntonie Van Leeuwenhoek10.1007/s10482-013-0057-42013Animals, Bacillus/*classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, China, DNA, Bacterial/genetics/metabolism, Fatty Acids/chemistry/metabolism, Intestines/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Smegmamorpha/*microbiologyMetabolism
Phylogeny26705002Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov.Liu Y, Lai Q, Du J, Shao ZInt J Syst Evol Microbiol10.1099/ijsem.0.0008562015
Metabolism27771954Biodegradation of Chlorpyrifos, Malathion, and Dimethoate by Three Strains of Bacteria Isolated from Pesticide-Polluted Soils in Sudan.Ishag AE, Abdelbagi AO, Hammad AM, Elsheikh EA, Elsaid OE, Hur JH, Laing MDJ Agric Food Chem10.1021/acs.jafc.6b033342016Bacillus/classification/genetics/isolation & purification/*metabolism, Bacillus cereus/classification/genetics/isolation & purification/*metabolism, Biodegradation, Environmental, Chlorpyrifos/*metabolism, Dimethoate/*metabolism, Malathion/*metabolism, Pesticides/*metabolism, Phylogeny, *Soil Microbiology, Soil Pollutants/*metabolism, SudanPhylogeny
Phylogeny29884123Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates.Tirumalai MR, Stepanov VG, Wunsche A, Montazari S, Gonzalez RO, Venkateswaran K, Fox GEBMC Microbiol10.1186/s12866-018-1191-y2018Bacillus/classification/*genetics, Bacillus pumilus/genetics, Bacterial Proteins/genetics, DNA Gyrase/genetics, Gene Order, *Genome, Bacterial, Molecular Sequence Annotation, Open Reading Frames, Phylogeny, Sequence Analysis, DNA/*methods, Spacecraft, Spores, Bacterial/classification/genetics/*isolation & purificationGenetics
Pathogenicity35305633Antimicrobial activity of Bacillus sp. isolated strains of wild honey.Hallaj-Nezhadi S, Hamdipour R, Shahrvirani M, Zare Tin R, Chapeland-Leclerc F, Ruprich-Robert G, Esnaashari S, Elyasi Far B, Dilmaghani ABMC Complement Med Ther10.1186/s12906-022-03551-y2022*Anti-Infective Agents/pharmacology, *Bacillus/genetics, Fungi, *Honey, Humans, RNA, Ribosomal, 16SPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8001Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19292)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19292
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22901Raquel Branquinho, Clara Sousa, Hugo Osório, Luís Meirinhos-Soares, João Lopes, João A. Carriço, Hans-Jürgen Busse, Amir Abdulmawjood, Günter Klein, Peter Kämpfer, Manuela E. Pintado, Luísa V. Peixe10.1099/ijs.0.067850-0Bacillus invictae sp. nov., isolated from a health productIJSEM 64: 3867-3876 201425171924
22902Masataka Satomi, Myron T. La Duc, Kasthuri Venkateswaran10.1099/ijs.0.64189-0Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfacesIJSEM 56: 1735-1740 200616902000
33297Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7151
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70920Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297138.1StrainInfo: A central database for resolving microbial strain identifiers
122346Curators of the CIPCollection of Institut Pasteur (CIP 109412)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109412