Strain identifier

BacDive ID: 12628

Type strain: Yes

Species: Microlunatus aurantiacus

Strain history: DSM 18424 <-- S.-K. Tang YIM 45721.

NCBI tax ID(s): 446786 (species)

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General

@ref: 7549

BacDive-ID: 12628

DSM-Number: 18424

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Microlunatus aurantiacus DSM 18424 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from rhizosphere soil.

NCBI tax id

  • NCBI tax id: 446786
  • Matching level: species

strain history

@refhistory
7549<- Shu-Kun Tang; YIM 45721
67770DSM 18424 <-- S.-K. Tang YIM 45721.

doi: 10.13145/bacdive12628.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus aurantiacus
  • full scientific name: Microlunatus aurantiacus Wang et al. 2008

@ref: 7549

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus aurantiacus

full scientific name: Microlunatus aurantiacus Wang et al. 2008

type strain: yes

Morphology

cell morphology

  • @ref: 32453
  • gram stain: positive
  • cell width: 0.9-1.3 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
19764Broom yellow (1032)10-14 daysISP 2
19764Broom yellow (1032)10-14 daysISP 3
19764Broom yellow (1032)10-14 daysISP 4
1976410-14 daysISP 5
1976410-14 daysISP 6
1976410-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19764noISP 2
19764noISP 3
19764noISP 4
19764noISP 5
19764noISP 6
19764noISP 7

pigmentation

  • @ref: 32453
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7549R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
19764ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19764ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19764ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19764ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19764ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19764ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
7549positivegrowth28mesophilic
19764positiveoptimum28mesophilic
32453positivegrowth15-37
32453positiveoptimum28mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
32453positivegrowth7-7.5
32453positiveoptimum7-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 32453
  • oxygen tolerance: aerobe

spore formation

  • @ref: 32453
  • spore formation: no

observation

@refobservation
32453aggregates in chains
67770quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3245330089acetate+carbon source
3245315963ribitol+carbon source
3245316449alanine+carbon source
3245322599arabinose+carbon source
3245329016arginine+carbon source
3245322653asparagine+carbon source
3245329987glutamate+carbon source
3245317754glycerol+carbon source
3245327570histidine+carbon source
3245329864mannitol+carbon source
3245337684mannose+carbon source
3245328053melibiose+carbon source
3245316634raffinose+carbon source
3245333942ribose+carbon source
3245317814salicin+carbon source
3245330911sorbitol+carbon source
3245317992sucrose+carbon source
3245326986threonine+carbon source
3245327082trehalose+carbon source
3245353424tween 20+carbon source
3245318222xylose+carbon source
3245317632nitrate+reduction

enzymes

@refvalueactivityec
32453catalase+1.11.1.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19764+++-++++-+++++++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7549rhizosphere soilYunnan ProvinceChinaCHNAsia
67770Rhizosphere soilYunnan ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host Body-Site#Plant#Rhizosphere
#Host#Plants

taxonmaps

  • @ref: 69479
  • File name: preview.99_12163.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_3710;97_4562;98_5854;99_12163&stattab=map
  • Last taxonomy: Microlunatus
  • 16S sequence: EF601828
  • Sequence Identity:
  • Total samples: 7462
  • soil counts: 2481
  • aquatic counts: 1755
  • animal counts: 3135
  • plant counts: 91

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
75491Risk group (German classification)
197641Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7549
  • description: Microlunatus aurantiacus strain YIM 45721 16S ribosomal RNA gene, partial sequence
  • accession: EF601828
  • length: 1517
  • database: ena
  • NCBI tax ID: 446786

GC content

@refGC-contentmethod
754970.9
6777070.9high performance liquid chromatography (HPLC)

External links

@ref: 7549

culture collection no.: DSM 18424, CCTCC AB 206067, JCM 16548, YIM 45721

straininfo link

  • @ref: 81842
  • straininfo: 402020

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18676472Microlunatus aurantiacus sp. nov., a novel actinobacterium isolated from a rhizosphere soil sample.Wang YX, Cai M, Zhi XY, Zhang YQ, Tang SK, Xu LH, Cui XL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.65518-02008Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Roots/*microbiology, Propionibacteriaceae/*classification/genetics/isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, Taxus/*microbiologyGenetics
Phylogeny19946055Microlunatus parietis sp. nov., isolated from an indoor wall.Kampfer P, Schafer J, Lodders N, Martin KInt J Syst Evol Microbiol10.1099/ijs.0.020115-02009Bacterial Typing Techniques, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, *Environmental Microbiology, Fatty Acids, Molecular Sequence Data, Mycolic Acids/analysis, Phospholipids/analysis, Phylogeny, Propionibacteriaceae/chemistry/*classification/genetics/*isolation & purification, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7549Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18424)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18424
19764Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM18424.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32453Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2867528776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
81842Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402020.1StrainInfo: A central database for resolving microbial strain identifiers