Strain identifier

BacDive ID: 1262

Type strain: Yes

Species: Halalkalibacter okhensis

Strain Designation: Kh10-101

Strain history: CIP <- 2006, JCM <- B. Patel: strain Kh10-101 <- S. Shingh

NCBI tax ID(s): 333138 (species)

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General

@ref: 16780

BacDive-ID: 1262

DSM-Number: 23308

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, motile, rod-shaped

description: Halalkalibacter okhensis Kh10-101 is an aerobe, Gram-positive, motile bacterium that was isolated from Indian saltpan.

NCBI tax id

  • NCBI tax id: 333138
  • Matching level: species

strain history

@refhistory
16780<- CIP <- JCM/RIKEN <- B. Patel, Griffith Univ., Nathan Campus, Australia <- S. P. Singh, Saurashtra Univ., Rajkot, India
67770B. K. C. Patel Kh10-101 <-- S. P. Singh.
121405CIP <- 2006, JCM <- B. Patel: strain Kh10-101 <- S. Shingh

doi: 10.13145/bacdive1262.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Halalkalibacter
  • species: Halalkalibacter okhensis
  • full scientific name: Halalkalibacter okhensis (Nowlan et al. 2006) Joshi et al. 2022

  • synonyms

    @refsynonym
    20215Alkalihalobacillus okhensis
    20215Bacillus okhensis

@ref: 16780

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Halalkalibacter

species: Halalkalibacter okhensis

full scientific name: Bacillus okhensis Nowlan et al. 2006

strain designation: Kh10-101

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31591positive02-03 µm0.6-0.8 µmrod-shapedyes
121405positiverod-shapedyes

pigmentation

  • @ref: 31591
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37492MEDIUM 678 - for Bacillus okhensisyesDistilled water make up to (1000.000 ml);Sodium chloride (50.000 g);Glucose (10.000 g);Yeast extract (5.000 g);Peptone (5.000 g);Potassium di-hydrogen phosphate(10.000 g)
121405CIP Medium 678yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=678

culture temp

@refgrowthtypetemperaturerange
31591positivegrowth25-40
31591positiveoptimum37mesophilic
37492positivegrowth37mesophilic
67770positivegrowth35mesophilic

culture pH

@refabilitytypepHPH range
31591positivegrowth07-10alkaliphile
31591positiveoptimum9

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31591aerobe
121405obligate aerobe

spore formation

  • @ref: 31591
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31591NaClpositivegrowth0-10 %
31591NaClpositiveoptimum5 %

observation

  • @ref: 31591
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3159122599arabinose+carbon source
3159128757fructose+carbon source
3159133984fucose+carbon source
3159128260galactose+carbon source
3159117234glucose+carbon source
3159137684mannose+carbon source
3159126546rhamnose+carbon source
3159133942ribose+carbon source
3159118222xylose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12140517632nitrate+reduction
12140516301nitrite-reduction

metabolite production

  • @ref: 121405
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31591catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121405oxidase+
121405gelatinase+
121405catalase+1.11.1.6
121405urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121405--++-------+-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121405------------------------+/-------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16780Indian saltpanvicinity of Port Okha (22° 15' N 69° 1' E)IndiaINDAsia
67770Saltpan in the vicinity of Port OkhaIndiaINDAsia
121405Indian saltpanIndiaINDAsia

isolation source categories

  • Cat1: #Condition
  • Cat2: #Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_2205.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_880;97_1028;98_1720;99_2205&stattab=map
  • Last taxonomy: Alkalihalobacillus okhensis
  • 16S sequence: DQ026060
  • Sequence Identity:
  • Total samples: 435
  • soil counts: 30
  • aquatic counts: 40
  • animal counts: 364
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
167801Risk group (German classification)
1214051Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16780
  • description: Bacillus okhensis strain Kh10-101 16S ribosomal RNA gene, partial sequence
  • accession: DQ026060
  • length: 1467
  • database: ena
  • NCBI tax ID: 333138

Genome sequences

  • @ref: 67770
  • description: Halalkalibacter okhensis Kh10-101
  • accession: GCA_000787375
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 333138

GC content

@refGC-contentmethod
1678041.6±1
3159141.6
6777041thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.147no
flagellatedyes83.774no
gram-positiveyes90.908yes
anaerobicno98.217yes
aerobicyes90.128yes
halophileyes63.529no
spore-formingyes89.2yes
thermophileno98.976no
glucose-utilyes91.103no
glucose-fermentno91.965no

External links

@ref: 16780

culture collection no.: DSM 23308, CIP 109247, JCM 13040, ATCC BAA 1137

straininfo link

  • @ref: 70918
  • straininfo: 290960

literature

  • Pubmed-ID: 34350093
  • title: Comparative genome analysis of Alkalihalobacillus okhensis Kh10-101( T) reveals insights into adaptive mechanisms for halo-alkali tolerance.
  • authors: Krishna PS, Raghunathan S, Prakash JSS
  • journal: 3 Biotech
  • DOI: 10.1007/s13205-021-02938-x
  • year: 2021

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
16780Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23308)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23308
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31591Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2788028776041
37492Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6967
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70918Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID290960.1StrainInfo: A central database for resolving microbial strain identifiers
121405Curators of the CIPCollection of Institut Pasteur (CIP 109247)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109247