Strain identifier

BacDive ID: 12618

Type strain: Yes

Species: Microlunatus okinawensis

Strain Designation: FB1, O4_4

Strain history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; O4_4

NCBI tax ID(s): 546873 (species)

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General

@ref: 15910

BacDive-ID: 12618

DSM-Number: 21744

keywords: 16S sequence, Bacteria, aerobe, coccus-shaped

description: Microlunatus okinawensis FB1 is an aerobe, coccus-shaped bacterium that was isolated from spider web.

NCBI tax id

  • NCBI tax id: 546873
  • Matching level: species

strain history

  • @ref: 15910
  • history: <- K. Iwai, BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd.; O4_4

doi: 10.13145/bacdive12618.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus okinawensis
  • full scientific name: Microlunatus okinawensis (Iwai et al. 2010) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Friedmanniella okinawensis

@ref: 15910

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus okinawensis

full scientific name: Microlunatus okinawensis (Iwai et al. 2010) Nouioui et al. 2018

strain designation: FB1, O4_4

type strain: yes

Morphology

cell morphology

  • @ref: 29168
  • cell width: 0.65 µm
  • cell shape: coccus-shaped

colony morphology

@refcolony colorincubation periodmedium used
21359Saffron yellow (1017)10-14 daysISP 2
21359Maize yellow (1006)10-14 daysISP 3
21359Maize yellow (1006)10-14 daysISP 4
21359Saffron yellow (1017)10-14 daysISP 5
21359Ivory (1014)10-14 daysISP 7
21359Ivory (1014)10-14 dayssuter with tyrosine
2135910-14 dayssuter without tyrosine

multicellular morphology

@refforms multicellular complexmedium name
21359noISP 2
21359noISP 3
21359noISP 4
21359noISP 5
21359noISP 7
21359noSuter with tyrosine
21359noSuter without tyrosine

pigmentation

  • @ref: 29168
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15910R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
21359ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21359ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21359ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21359ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21359ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21359suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21359suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15910positivegrowth22-28
29168positivegrowth18-32
29168positiveoptimum25mesophilic

culture pH

@refabilitytypepHPH range
29168positivegrowth05-09alkaliphile
29168positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 29168
  • oxygen tolerance: aerobe

halophily

  • @ref: 29168
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-4 %

observation

  • @ref: 29168
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2135917234glucose-
2135922599arabinose-
2135917992sucrose-
2135918222xylose-
2135917268myo-inositol+
2135937684mannose+
2135928757fructose-
2135926546rhamnose+
2135916634raffinose-
2135962968cellulose-
2916822599arabinose+carbon source
2916828757fructose+carbon source
2916828260galactose+carbon source
2916817234glucose+carbon source
2916829864mannitol+carbon source
2916817268myo-inositol+carbon source
2916816634raffinose+carbon source
2916826546rhamnose+carbon source
2916833942ribose+carbon source
2916817992sucrose+carbon source
2916818222xylose+carbon source

enzymes

@refvalueactivityec
29168catalase+1.11.1.6
29168gelatinase+
29168urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382leucine arylamidase+3.4.11.1
68382trypsin+3.4.21.4
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21359+++/-+/-++/-+/-++/-+-++-+++++

Isolation, sampling and environmental information

isolation

  • @ref: 15910
  • sample type: spider web
  • geographic location: Okinawa Prefecture
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids

Safety information

risk assessment

  • @ref: 15910
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15910
  • description: Friedmanniella okinawensis gene for 16S rRNA, partial sequence
  • accession: AB445455
  • length: 1417
  • database: ena
  • NCBI tax ID: 546873

GC content

@refGC-contentmethod
1591074high performance liquid chromatography (HPLC)
2916874

External links

@ref: 15910

culture collection no.: DSM 21744, NBRC 104966, KY 13159

straininfo link

  • @ref: 81832
  • straininfo: 399812

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19648329Friedmanniella luteola sp. nov., Friedmanniella lucida sp. nov., Friedmanniella okinawensis sp. nov. and Friedmaniella sagamiharensis sp. nov., isolated from spiders.Iwai K, Aisaka K, Suzuki MInt J Syst Evol Microbiol10.1099/ijs.0.007815-02009Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Spiders/*microbiologyMetabolism
Phylogeny22941301Friedmanniella flava sp. nov., a soil actinomycete.Zhang X, Zhang J, Zhang Y, Xin Y, He HInt J Syst Evol Microbiol10.1099/ijs.0.043984-02012Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15910Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21744)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21744
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21359Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM21744.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29168Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2559228776041
68382Automatically annotated from API zym
81832Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399812.1StrainInfo: A central database for resolving microbial strain identifiers