Strain identifier

BacDive ID: 1225

Type strain: Yes

Species: Rossellomorea aquimaris

Strain Designation: TF-12

Strain history: CIP <- 2003, JCM <- 2002, Y.H. Park: strain TF-12

NCBI tax ID(s): 189382 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6324

BacDive-ID: 1225

DSM-Number: 16205

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-variable, motile, rod-shaped, colony-forming

description: Rossellomorea aquimaris TF-12 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from sea water of a tidal flat.

NCBI tax id

  • NCBI tax id: 189382
  • Matching level: species

strain history

@refhistory
6324<- F. G. Priest, Univ. Edinburgh <- J.-H. Yoon; TF-12
67770Y.-H. Park TF-12.
116458CIP <- 2003, JCM <- 2002, Y.H. Park: strain TF-12

doi: 10.13145/bacdive1225.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Rossellomorea
  • species: Rossellomorea aquimaris
  • full scientific name: Rossellomorea aquimaris (Yoon et al. 2003) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus aquimaris

@ref: 6324

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Rossellomorea

species: Rossellomorea aquimaris

full scientific name: Rossellomorea aquimaris (Yoon et al. 2003) Gupta et al. 2020

strain designation: TF-12

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23314variable1.2-3.5 µm0.5-0.7 µmrod-shapedyesperitrichous
116458positiverod-shapedyes

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium usedhemolysis ability
233142-4 mmpale orange-yellowcircular3-4 daysMA
1164581

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6324BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23314Marine agar (MA)yes
32894Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116458CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13
116458CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6324positivegrowth30mesophilic
23314positivegrowth10-44
23314nogrowth4psychrophilic
23314nogrowth>45thermophilic
23314positiveoptimum30-37mesophilic
32894positivegrowth30mesophilic
67770positivegrowth30mesophilic
116458positivegrowth10-45
116458nogrowth55thermophilic

culture pH

@refabilitytypepH
23314positiveoptimum6.0-7.0
23314nogrowth9.0
23314nogrowth4.5
116458nogrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23314aerobe
116458obligate aerobe

spore formation

@refspore descriptiontype of sporespore formation
23314ellipsoidal, central, large swollen sporangiaendosporeyes
116458yes

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23314NaClpositiveoptimum2-5 %
23314NaClinconsistentgrowth0 %
23314NaClpositivemaximum18 %
23314moderately halophilic
116458NaClnogrowth0 %
116458NaClnogrowth2 %
116458NaClnogrowth4 %
116458NaClnogrowth6 %
116458NaClnogrowth8 %
116458NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23314168082-dehydro-D-gluconate-builds acid from
23314174265-dehydro-D-gluconate-builds acid from
2331415963ribitol-builds acid from
2331427613amygdalin-builds acid from
2331418305arbutin-builds acid from
2331417108D-arabinose-builds acid from
2331418333D-arabitol-builds acid from
2331417057cellobiose-builds acid from
2331428847D-fucose-builds acid from
2331412936D-galactose-builds acid from
2331462318D-lyxose-builds acid from
2331416899D-mannitol-builds acid from
2331416024D-mannose-builds acid from
233146731melezitose-builds acid from
2331416634raffinose-builds acid from
2331417924D-sorbitol-builds acid from
2331416443D-tagatose-builds acid from
2331432528turanose-builds acid from
2331465327D-xylose-builds acid from
2331416813galactitol-builds acid from
2331417113erythritol-builds acid from
233144853esculin-builds acid from
2331424265gluconate-builds acid from
2331428087glycogen-builds acid from
2331415443inulin-builds acid from
2331430849L-arabinose-builds acid from
2331418403L-arabitol-builds acid from
2331418287L-fucose-builds acid from
2331462345L-rhamnose-builds acid from
2331417266L-sorbose-builds acid from
2331465328L-xylose-builds acid from
2331417716lactose-builds acid from
2331428053melibiose-builds acid from
23314320061methyl alpha-D-glucopyranoside-builds acid from
2331443943methyl alpha-D-mannoside-builds acid from
2331474863methyl beta-D-xylopyranoside-builds acid from
2331417268myo-inositol-builds acid from
23314506227N-acetylglucosamine-builds acid from
2331417814salicin-builds acid from
2331417164stachyose-builds acid from
2331428017starch-builds acid from
2331417151xylitol-builds acid from
233144853esculin-hydrolysis
2331417368hypoxanthine-hydrolysis
2331428017starch-hydrolysis
2331453426tween 80-hydrolysis
2331418186tyrosine-hydrolysis
2331415318xanthine-hydrolysis
23314174265-dehydro-D-gluconate+builds acid from
2331418305arbutin+builds acid from
2331417057cellobiose+builds acid from
2331415824D-fructose+builds acid from
2331417634D-glucose+builds acid from
2331416899D-mannitol+builds acid from
2331416024D-mannose+builds acid from
2331416988D-ribose+builds acid from
2331416551D-trehalose+builds acid from
2331465327D-xylose+builds acid from
233144853esculin+builds acid from
2331428066gentiobiose+builds acid from
2331417754glycerol+builds acid from
2331428087glycogen+builds acid from
2331417306maltose+builds acid from
2331428053melibiose+builds acid from
2331443943methyl alpha-D-mannoside+builds acid from
2331417814salicin+builds acid from
2331417164stachyose+builds acid from
2331428017starch+builds acid from
2331417992sucrose+builds acid from
23314casein+hydrolysis
233144853esculin+hydrolysis
2331428017starch+hydrolysis
2331453426tween 80+hydrolysis
2331412936D-galactose+/-builds acid from
2331416634raffinose+/-builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
11645816947citrate-carbon source
1164584853esculin-hydrolysis
116458606565hippurate-hydrolysis
11645817632nitrate-reduction
11645816301nitrite-reduction
11645817632nitrate-respiration

metabolite production

  • @ref: 116458
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11645815688acetoin-
11645817234glucose+

enzymes

@refvalueactivityec
23314catalase+1.11.1.6
23314cytochrome oxidase-1.9.3.1
23314urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
116458oxidase-
116458beta-galactosidase+3.2.1.23
116458alcohol dehydrogenase-1.1.1.1
116458gelatinase+/-
116458amylase+
116458DNase+
116458caseinase+3.4.21.50
116458catalase+1.11.1.6
116458tween esterase-
116458gamma-glutamyltransferase+2.3.2.2
116458lecithinase-
116458lipase+
116458lysine decarboxylase-4.1.1.18
116458ornithine decarboxylase-4.1.1.17
116458protease+
116458urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116458-+-+-----+--------+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116458+/----+-----++------------+--+--+/-+---++------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116458------------------------------------------------------------+--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6324sea water of a tidal flatYellow SeaRepublic of KoreaKORAsia
67770Seawater of a tidal flat of the Yellow SeaRepublic of KoreaKORAsia
116458Environment, Tidal flatYellow seaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Tidal flat

taxonmaps

  • @ref: 69479
  • File name: preview.99_3166.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1652;97_1976;98_2430;99_3166&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: AF483625
  • Sequence Identity:
  • Total samples: 2332
  • soil counts: 945
  • aquatic counts: 996
  • animal counts: 294
  • plant counts: 97

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63241Risk group (German classification)
1164581Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6324
  • description: Bacillus aquaemaris strain TF-12 16S ribosomal RNA gene, partial sequence
  • accession: AF483625
  • length: 1507
  • database: ena
  • NCBI tax ID: 1073842

Genome sequences

  • @ref: 67770
  • description: Rossellomorea aquimaris TF-12 TF12
  • accession: GCA_001648555
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1073842

GC content

@refGC-contentmethod
632438high performance liquid chromatography (HPLC)
2331438.00high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes88.596yes
gram-positiveyes83.28yes
anaerobicno98.427yes
halophileyes76.172no
spore-formingyes94.195yes
glucose-utilyes91.383no
motileyes89.345yes
aerobicyes92.149yes
thermophileno99.029no
glucose-fermentno93.316no

External links

@ref: 6324

culture collection no.: DSM 16205, JCM 11545, KCCM 41589, CIP 108136

straininfo link

  • @ref: 70881
  • straininfo: 81585

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130010Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea.Yoon JH, Kim IG, Kang KH, Oh TK, Park YHInt J Syst Evol Microbiol10.1099/ijs.0.02365-02003Bacillus/classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Korea, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Species SpecificityGenetics
Phylogeny16902027Bacillus seohaeanensis sp. nov., a halotolerant bacterium that contains L-lysine in its cell wall.Lee JC, Lim JM, Park DJ, Jeon CO, Li WJ, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.64237-02006Bacillus/chemistry/*classification/isolation & purification/physiology, Base Composition, Cell Wall/chemistry, Culture Media, Cyanoacrylates/analysis, DNA, Bacterial/genetics, Fatty Acids/analysis, *Industrial Microbiology, Lysine/analysis/metabolism, Molecular Sequence Data, Peptidoglycan/analysis/metabolism, Phenotype, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride, Species Specificity, Temperature, Vitamin K 2/analogs & derivativesCultivation
Phylogeny24860111Bacillus oryzaecorticis sp. nov., a moderately halophilic bacterium isolated from rice husks.Hong SW, Kwon SW, Kim SJ, Kim SY, Kim JJ, Lee JS, Oh MH, Kim AJ, Chung KSInt J Syst Evol Microbiol10.1099/ijs.0.058768-02014Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/chemistryGenetics
Phylogeny25100188Bacillus haikouensis sp. nov., a facultatively anaerobic halotolerant bacterium isolated from a paddy soil.Li J, Yang G, Lu Q, Zhao Y, Zhou SAntonie Van Leeuwenhoek10.1007/s10482-014-0248-72014Aerobiosis, Anaerobiosis, Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Vitamin K 2/analysisGenetics
Phylogeny30767851Bacillus salacetis sp. nov., a slightly halophilic bacterium from Thai shrimp paste (Ka-pi).Daroonpunt R, Yiamsombut S, Sitdhipol J, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0032862019Bacillus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Food Microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6324Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16205)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16205
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23314Jung-Hoon Yoon, In-Gi Kim, Kook Hee Kang, Tae-Kwang Oh, Yong-Ha Park10.1099/ijs.0.02365-0Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in KoreaIJSEM 53: 1297-1303 200313130010
32894Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5725
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70881Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID81585.1StrainInfo: A central database for resolving microbial strain identifiers
116458Curators of the CIPCollection of Institut Pasteur (CIP 108136)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108136