Strain identifier
BacDive ID: 12001
Type strain: 
Species: Sporosarcina contaminans
Strain history: <- P. Kämpfer, Univ. Giessen, Germany; CCUG 53915
NCBI tax ID(s): 633403 (species)
version 8.1 (current version)
General
@ref: 16200
BacDive-ID: 12001
DSM-Number: 22204
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Sporosarcina contaminans DSM 22204 is an aerobe, spore-forming, mesophilic bacterium that was isolated from industrial clean room floor.
NCBI tax id
- NCBI tax id: 633403
- Matching level: species
strain history
- @ref: 16200
- history: <- P. Kämpfer, Univ. Giessen, Germany; CCUG 53915
doi: 10.13145/bacdive12001.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Caryophanaceae
- genus: Sporosarcina
- species: Sporosarcina contaminans
- full scientific name: Sporosarcina contaminans Kämpfer et al. 2010
@ref: 16200
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Caryophanaceae
genus: Sporosarcina
species: Sporosarcina contaminans
full scientific name: Sporosarcina contaminans Kämpfer et al. 2010
type strain: yes
Morphology
cell morphology
- @ref: 29501
- gram stain: positive
- cell length: 2.5 µm
- cell width: 0.9 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 29501
- production: yes
Culture and growth conditions
culture medium
- @ref: 16200
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16200 | positive | growth | 28 | mesophilic |
| 29501 | positive | growth | 15-50 | |
| 29501 | positive | optimum | 30 | mesophilic |
| 60078 | positive | growth | 37 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29501 | positive | growth | 6.5-10.5 | alkaliphile |
| 29501 | positive | optimum | 7.5 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 29501 | aerobe |
| 60078 | aerobe |
spore formation
- @ref: 29501
- spore formation: yes
halophily
- @ref: 29501
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: <9 %
murein
- @ref: 16200
- murein short key: A11.33
- type: A4alpha L-Lys-D-Glu
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29501 | 25115 | malate | + | carbon source |
| 29501 | 15361 | pyruvate | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29501 | catalase | + | 1.11.1.6 |
| 29501 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | sampling date |
|---|---|---|---|---|---|---|
| 16200 | industrial clean room floor | Göteborg | Sweden | SWE | Europe | |
| 60078 | Contaminant,industrial clean room floor | Sweden | SWE | Europe | 2006-12-01 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Built environment | #Clean room |
| #Engineered | #Industrial |
taxonmaps
- @ref: 69479
- File name: preview.99_86093.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1276;97_1516;98_1846;99_86093&stattab=map
- Last taxonomy: Sporosarcina contaminans
- 16S sequence: FN298444
- Sequence Identity:
- Total samples: 157
- soil counts: 35
- aquatic counts: 4
- animal counts: 118
Safety information
risk assessment
- @ref: 16200
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16200
- description: Sporosarcina contaminans partial 16S rRNA gene, type strain CCUG 53915T
- accession: FN298444
- length: 1463
- database: ena
- NCBI tax ID: 633403
External links
@ref: 16200
culture collection no.: DSM 22204, CCUG 53915
straininfo link
- @ref: 81216
- straininfo: 303578
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19667369 | Sporosarcina contaminans sp. nov. and Sporosarcina thermotolerans sp. nov., two endospore-forming species. | Kampfer P, Falsen E, Lodders N, Schumann P | Int J Syst Evol Microbiol | 10.1099/ijs.0.014423-0 | 2009 | Blood/microbiology, Fatty Acids/analysis, Gram-Positive Endospore-Forming Rods/classification/*genetics/isolation & purification/metabolism, Humans, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride/metabolism, Temperature | Metabolism |
| Phylogeny | 24989328 | Sporosarcina siberiensis sp. nov., isolated from the East Siberian Sea. | Zhang G, Ren H, Chen X, Zhang Y, Yang Y, Wang S, Jiang Y | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0217-1 | 2014 | Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Siberia, Sodium Chloride/metabolism, Sporosarcina/*classification/genetics/*isolation & purification/physiology, Temperature, Vitamin K 2/analysis | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 16200 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22204) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22204 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29501 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25901 | 28776041 | |
| 60078 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 53915) | https://www.ccug.se/strain?id=53915 | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 81216 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID303578.1 | StrainInfo: A central database for resolving microbial strain identifiers |