Strain identifier

BacDive ID: 11944

Type strain: Yes

Species: Singulisphaera rosea

Strain Designation: S26

Strain history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S26 <- I. S. Kulichevskaya

NCBI tax ID(s): 643672 (species)

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General

@ref: 16668

BacDive-ID: 11944

DSM-Number: 23044

keywords: 16S sequence, Bacteria, aerobe, mesophilic, coccus-shaped

description: Singulisphaera rosea S26 is an aerobe, mesophilic, coccus-shaped bacterium that was isolated from Sphagnum peat.

NCBI tax id

  • NCBI tax id: 643672
  • Matching level: species

strain history

  • @ref: 16668
  • history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; S26 <- I. S. Kulichevskaya

doi: 10.13145/bacdive11944.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/planctomycetota
  • domain: Bacteria
  • phylum: Planctomycetota
  • class: Planctomycetia
  • order: Isosphaerales
  • family: Isosphaeraceae
  • genus: Singulisphaera
  • species: Singulisphaera rosea
  • full scientific name: Singulisphaera rosea Kulichevskaya et al. 2012

@ref: 16668

domain: Bacteria

phylum: Planctomycetes

class: Planctomycetia

order: Planctomycetales

family: Isosphaeraceae

genus: Singulisphaera

species: Singulisphaera rosea

full scientific name: Singulisphaera rosea Kulichevskaya et al. 2012

strain designation: S26

type strain: yes

Morphology

cell morphology

  • @ref: 29910
  • cell width: 1.8-3.2 µm
  • cell shape: coccus-shaped
  • motility: no

pigmentation

  • @ref: 29910
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 16668
  • name: SINGULOSPHAERA MEDIUM (DSMZ Medium 1144)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1144
  • composition: Name: SINGULISPHAERA MEDIUM (DSMZ Medium 1144) Composition: Agar 18.0 g/l N-Acetylglucosamine 1.0 g/l MgSO4 x 7 H2O 0.594 g/l Nitrilotriacetic acid 0.2 g/l Na-ampicillin 0.2 g/l Yeast extract 0.1 g/l Peptone 0.1 g/l KH2PO4 0.1 g/l CaCl2 x 2 H2O 0.0668 g/l ZnSO4 x 7 H2O 0.001095 g/l FeSO4 x 7 H2O 0.0005 g/l Na-EDTA 0.00025 g/l (NH4)6Mo7O24 x 4 H2O 0.000185 g/l MnSO4 x H2O 0.000154 g/l CuSO4 x 5 H2O 3.92e-05 g/l Co(NO3)2 x 6 H2O 2.48e-05 g/l Na2B4O7 x 10 H2O 1.77e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16668positivegrowth28mesophilic
29910positivegrowth04-33
29910positiveoptimum20-26

culture pH

@refabilitytypepHPH range
29910positivegrowth3.2-7.1acidophile
29910positiveoptimum4.8-5.0

Physiology and metabolism

oxygen tolerance

  • @ref: 29910
  • oxygen tolerance: aerobe

halophily

  • @ref: 29910
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <1 %

observation

  • @ref: 29910
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2991022599arabinose+carbon source
2991029016arginine+carbon source
2991017057cellobiose+carbon source
2991016947citrate+carbon source
2991028757fructose+carbon source
2991028260galactose+carbon source
2991017234glucose+carbon source
2991024996lactate+carbon source
2991017716lactose+carbon source
2991025115malate+carbon source
2991017306maltose+carbon source
2991029864mannitol+carbon source
2991037684mannose+carbon source
2991028053melibiose+carbon source
2991015361pyruvate+carbon source
2991016634raffinose+carbon source
2991026546rhamnose+carbon source
2991033942ribose+carbon source
2991017814salicin+carbon source
2991030911sorbitol+carbon source
2991030031succinate+carbon source
2991017992sucrose+carbon source
2991018222xylose+carbon source

enzymes

  • @ref: 29910
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

  • @ref: 16668
  • sample type: Sphagnum peat
  • host species: Sphagnum
  • geographic location: Tver region (56° 27' N 32° 58' E)
  • country: Russia
  • origin.country: RUS
  • continent: Europe
  • latitude: 56.45
  • longitude: 32.9667

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Peat moss

Safety information

risk assessment

  • @ref: 16668
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16668
  • description: Singulisphaera rosea partial 16S rRNA gene, type strain S26T
  • accession: FN391026
  • length: 1431
  • database: ena
  • NCBI tax ID: 643672

GC content

@refGC-contentmethod
1666862.2thermal denaturation, midpoint method (Tm)
1666858.0
2991062.2

External links

@ref: 16668

culture collection no.: DSM 23044, VKM B-2599

straininfo link

  • @ref: 81157
  • straininfo: 403807

literature

  • topic: Phylogeny
  • Pubmed-ID: 21335501
  • title: Singulisphaera rosea sp. nov., a planctomycete from acidic Sphagnum peat, and emended description of the genus Singulisphaera.
  • authors: Kulichevskaya IS, Detkova EN, Bodelier PLE, Rijpstra WIC, Sinninghe Damste JS, Dedysh SN
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.025924-0
  • year: 2011
  • mesh: Aerobiosis, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Pigments, Biological/metabolism, Planctomycetales/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, *Soil Microbiology, Sphagnopsida/*microbiology, Temperature
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16668Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23044)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23044
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29910Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2628228776041
81157Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403807.1StrainInfo: A central database for resolving microbial strain identifiers