Strain identifier
BacDive ID: 11760
Type strain: 
Species: Cryptanaerobacter phenolicus
Strain Designation: LR7.2
Strain history: <- P. Juteau, Université du Québec, INRS-Institut Armand-Frappier, Quebec, Canada; LR7.2 <- L. Racine {2000}
NCBI tax ID(s): 238083 (species)
version 9 (current version)
General
@ref: 6118
BacDive-ID: 11760
DSM-Number: 15808
keywords: 16S sequence, Bacteria, anaerobe, Gram-positive, rod-shaped
description: Cryptanaerobacter phenolicus LR7.2 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from methanogenic consortium that resulted from the mixture of swamp water, sewage sludge, swine waste and soil.
NCBI tax id
- NCBI tax id: 238083
- Matching level: species
strain history
- @ref: 6118
- history: <- P. Juteau, Université du Québec, INRS-Institut Armand-Frappier, Quebec, Canada; LR7.2 <- L. Racine {2000}
doi: 10.13145/bacdive11760.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Peptococcaceae
- genus: Cryptanaerobacter
- species: Cryptanaerobacter phenolicus
- full scientific name: Cryptanaerobacter phenolicus Juteau et al. 2005
@ref: 6118
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Peptococcaceae
genus: Cryptanaerobacter
species: Cryptanaerobacter phenolicus
full scientific name: Cryptanaerobacter phenolicus Juteau et al. 2005
strain designation: LR7.2
type strain: yes
Morphology
cell morphology
- @ref: 30077
- gram stain: positive
- cell length: 2 µm
- cell width: 1 µm
- cell shape: rod-shaped
colony morphology
- @ref: 6118
- incubation period: 8-14 days
pigmentation
- @ref: 30077
- production: yes
Culture and growth conditions
culture medium
- @ref: 6118
- name: CRYPTOANAEROBACTER MEDIUM (DSMZ Medium 1022)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1022
- composition: Name: CRYPTOANAEROBACTER MEDIUM (DSMZ Medium 1022) Composition: Yeast extract 4.98008 g/l NaHCO3 3.98406 g/l Casamino acids 0.996016 g/l 4-Hydroxybenzoic acid 0.448207 g/l K2HPO4 0.398406 g/l NH4Cl 0.398406 g/l MgCl2 x 6 H2O 0.0796813 g/l CaCl2 x 2 H2O 0.059761 g/l HCl 0.00498008 g/l FeCl2 x 4 H2O 0.00298805 g/l NaOH 0.000498008 g/l Sodium resazurin 0.000498008 g/l CoCl2 x 6 H2O 0.000378486 g/l MnCl2 x 4 H2O 0.000199203 g/l ZnCl2 0.000139442 g/l Pyridoxine hydrochloride 9.96016e-05 g/l Na2MoO4 x 2 H2O 7.17131e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l p-Aminobenzoic acid 4.98008e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l Nicotinic acid 4.98008e-05 g/l Riboflavin 4.98008e-05 g/l NiCl2 x 6 H2O 4.78088e-05 g/l Folic acid 1.99203e-05 g/l Biotin 1.99203e-05 g/l H3BO3 1.19522e-05 g/l Na2WO4 x 2 H2O 3.98406e-06 g/l CuCl2 x 2 H2O 3.98406e-06 g/l Na2SeO3 x 5 H2O 2.98805e-06 g/l Vitamin B12 9.96016e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 6118 | positive | growth | 37 |
| 30077 | positive | growth | 30-37 |
| 30077 | positive | optimum | 33.5 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30077 | positive | growth | 7.5-8 |
| 30077 | positive | optimum | 7.75 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 6118 | anaerobe |
| 30077 | anaerobe |
spore formation
- @ref: 30077
- spore formation: no
Isolation, sampling and environmental information
isolation
- @ref: 6118
- sample type: methanogenic consortium that resulted from the mixture of swamp water, sewage sludge, swine waste and soil
- geographic location: province of Québec
- country: Canada
- origin.country: CAN
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Waste | #Sewage sludge |
| #Environmental | #Terrestrial | #Wetland (Swamp) |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
| #Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_50151.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1116;96_13855;97_23514;98_36056;99_50151&stattab=map
- Last taxonomy: Cryptanaerobacter phenolicus subclade
- 16S sequence: AY327251
- Sequence Identity:
- Total samples: 293
- soil counts: 37
- aquatic counts: 180
- animal counts: 7
- plant counts: 69
Safety information
risk assessment
- @ref: 6118
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6118
- description: Cryptanaerobacter phenolicus strain LR7.2 16S ribosomal RNA gene, complete sequence
- accession: AY327251
- length: 1529
- database: nuccore
- NCBI tax ID: 238083
GC content
| @ref | GC-content |
|---|---|
| 6118 | 51.0 |
| 30077 | 51 |
External links
@ref: 6118
culture collection no.: DSM 15808, ATCC BAA-820
straininfo link
- @ref: 80978
- straininfo: 135108
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15653882 | Cryptanaerobacter phenolicus gen. nov., sp. nov., an anaerobe that transforms phenol into benzoate via 4-hydroxybenzoate. | Juteau P, Cote V, Duckett MF, Beaudet R, Lepine F, Villemur R, Bisaillon JG | Int J Syst Evol Microbiol | 10.1099/ijs.0.02914-0 | 2005 | Anaerobiosis, Animals, DNA, Bacterial/analysis, Molecular Sequence Data, Parabens/*metabolism, Peptococcaceae/*classification/*growth & development/isolation & purification/metabolism, Phenol/*metabolism, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/microbiology, Swine, Water Microbiology | Metabolism |
| Phylogeny | 16014504 | The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum. | de Bok FAM, Harmsen HJM, Plugge CM, de Vries MC, Akkermans ADL, de Vos WM, Stams AJM | Int J Syst Evol Microbiol | 10.1099/ijs.0.02880-0 | 2005 | Anaerobiosis, *Bioreactors, Culture Media, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, In Situ Hybridization, Fluorescence, Industrial Waste, Methanospirillum/*growth & development, Molecular Sequence Data, Oxidation-Reduction, Peptococcaceae/*classification/genetics/*growth & development/isolation & purification, Phenotype, Propionates/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, Fluid | Cultivation |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6118 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15808) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15808 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30077 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26434 | 28776041 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 80978 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID135108.1 | StrainInfo: A central database for resolving microbial strain identifiers |