Strain identifier
BacDive ID: 11707
Type strain:
Species: Haemophilus parainfluenzae
Strain Designation: Wound
Strain history: CIP <- 1986, NCTC <- 1949, A.B. Rosher, London, UK: strain Wound <- Glenson-White
NCBI tax ID(s): 888828 (strain), 729 (species)
General
@ref: 3450
BacDive-ID: 11707
DSM-Number: 8978
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic
description: Haemophilus parainfluenzae Wound is a microaerophile, mesophilic bacterium that was isolated from septic finger.
NCBI tax id
NCBI tax id | Matching level |
---|---|
888828 | strain |
729 | species |
strain history
@ref | history |
---|---|
3450 | <- ATCC; ATCC 33392 <- NCTC; NCTC 7857 <- A.B. Rosher; |
67771 | <- CCUG <- R Mutters, Marburg, Germany <- NCTC <- AB Rosher, London, 1949 <- Glesson-White |
121563 | CIP <- 1986, NCTC <- 1949, A.B. Rosher, London, UK: strain Wound <- Glenson-White |
doi: 10.13145/bacdive11707.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pasteurellales
- family: Pasteurellaceae
- genus: Haemophilus
- species: Haemophilus parainfluenzae
- full scientific name: Haemophilus parainfluenzae Rivers 1922 (Approved Lists 1980)
@ref: 3450
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pasteurellales
family: Pasteurellaceae
genus: Haemophilus
species: Haemophilus parainfluenzae
full scientific name: Haemophilus parainfluenzae Rivers 1922
strain designation: Wound
type strain: yes
Morphology
cell morphology
@ref | motility | gram stain | cell shape |
---|---|---|---|
67771 | no | ||
67771 | negative | ||
121563 | no | negative | rod-shaped |
colony morphology
@ref | incubation period |
---|---|
3450 | 1-2 days |
121563 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3450 | HAEMOPHILUS MEDIUM (DSMZ Medium 804) | yes | https://mediadive.dsmz.de/medium/804 | Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water |
33555 | MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml) | |
121563 | CIP Medium 10 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3450 | positive | growth | 37 | mesophilic |
33555 | positive | growth | 37 | mesophilic |
45838 | positive | growth | 37 | mesophilic |
67771 | positive | growth | 37 | mesophilic |
121563 | positive | growth | 25-41 | |
121563 | no | growth | 15 | psychrophilic |
121563 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
3450 | microaerophile |
45838 | microaerophile |
67771 | microaerophile |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121563 | 4853 | esculin | - | hydrolysis |
121563 | 606565 | hippurate | - | hydrolysis |
121563 | 17632 | nitrate | + | reduction |
121563 | 16301 | nitrite | - | reduction |
68377 | 17634 | D-glucose | + | builds acid from |
68377 | 27897 | tryptophan | - | energy source |
antibiotic resistance
- @ref: 121563
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68377 | 35581 | indole | no |
121563 | 35581 | indole | no |
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
3450 | catalase | + | 1.11.1.6 |
3450 | cytochrome-c oxidase | + | 1.9.3.1 |
68377 | tryptophan deaminase | - | 4.1.99.1 |
121563 | oxidase | - | |
121563 | beta-galactosidase | + | 3.2.1.23 |
121563 | alcohol dehydrogenase | - | 1.1.1.1 |
121563 | catalase | - | 1.11.1.6 |
121563 | gamma-glutamyltransferase | - | 2.3.2.2 |
121563 | lysine decarboxylase | - | 4.1.1.18 |
121563 | ornithine decarboxylase | + | 4.1.1.17 |
121563 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
@ref fatty acid percentage ECL 45838 C14:0 18.5 14 45838 C16:0 29.7 16 45838 C18:0 0.6 18 45838 C13:0 ISO 2OH 0.4 13.814 45838 C14:0 3OH/C16:1 ISO I 7.4 15.485 45838 C14:1 CIS 7 DMA 0.5 14.291 45838 C15:0 ISO 3OH 0.7 16.135 45838 C16:1 ω7c 37.4 15.819 45838 C16:1 ω7c DMA 0.8 16.288 45838 C16:1 ω9c 0.2 15.774 45838 C18:1 ω7c /12t/9t 0.9 17.824 45838 C18:1 ω9c 1.1 17.769 45838 C18:2 ω6,9c/C18:0 ANTE 1.5 17.724 45838 unknown 14.503 0.4 14.503 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
@ref fatty acid percentage ECL 45838 C14:0 21 14 45838 C16:0 26 16 45838 C18:0 0.4 18 45838 C13:0 ISO 2OH 0.5 13.814 45838 C14:0 3OH/C16:1 ISO I 9.8 15.485 45838 C16:1 ω7c 39.5 15.819 45838 C18:1 ω9c 1.1 17.769 45838 C18:2 ω6,9c/C18:0 ANTE 1.3 17.724 45838 unknown 14.503 0.5 14.503 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121563 | - | + | - | - | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API NH
@ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3450 | - | + | + | + | + | + | - | - | + | - | - | - | - |
3450 | + | + | + | + | + | - | - | + | + | - | + | - | |
3450 | + | + | + | + | + | - | +/- | + | +/- | - | - | - | |
3450 | + | + | + | + | + | - | - | + | + | - | - | - | - |
3450 | + | + | + | + | + | - | - | + | + | - | + | - | - |
3450 | - | + | - | - | - | - | - | - | + | + | - | - | - |
3450 | + | + | + | + | + | - | - | + | + | - | + | - | |
3450 | + | + | + | + | + | - | +/- | + | +/- | - | - | - | |
3450 | - | + | + | + | + | + | - | - | + | + | - | - | - |
3450 | - | + | + | + | + | + | - | - | + | + | - | + | - |
3450 | + | + | + | + | + | + | - | - | + | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
3450 | septic finger | ||||
45838 | Human wound,septic finger | London? | United Kingdom | GBR | Europe |
67771 | From human wound, septic finger | ||||
121563 | Animal, Septic finger |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Disease | |
#Host Body-Site | #Limb | #Hand |
taxonmaps
- @ref: 69479
- File name: preview.99_403.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_273;97_301;98_335;99_403&stattab=map
- Last taxonomy: Haemophilus parainfluenzae subclade
- 16S sequence: AY362908
- Sequence Identity:
- Total samples: 128855
- soil counts: 1672
- aquatic counts: 4078
- animal counts: 121644
- plant counts: 1461
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
3450 | 2 | Risk group (German classification) |
121563 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Haemophilus parainfluenzae strain DSM 8978 16S ribosomal RNA gene, partial sequence | HQ012020 | 934 | ena | 888828 |
20218 | Haemophilus parainfluenzae ATCC 33392 16S ribosomal RNA gene, partial sequence | M75081 | 1483 | ena | 888828 |
3450 | Haemophilus parainfluenzae ATCC 33392 strain CCUG 12836 16S ribosomal RNA gene, partial sequence | AY362908 | 1362 | ena | 888828 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Haemophilus parainfluenzae ATCC 33392 | GCA_000191405 | scaffold | ncbi | 888828 |
66792 | Haemophilus parainfluenzae ATCC 33392 NCTC 7857 | GCA_900450845 | contig | ncbi | 888828 |
66792 | Haemophilus parainfluenzae ATCC 33392 | 888828.3 | wgs | patric | 888828 |
66792 | Haemophilus parainfluenzae ATCC 33392 | 888828.6 | wgs | patric | 888828 |
66792 | Haemophilus parainfluenzae ATCC 33392 strain NCTC 7857 | 888828.150 | wgs | patric | 888828 |
66792 | Haemophilus parainfluenzae strain FDAARGOS_1000 | 729.2141 | complete | patric | 729 |
66792 | Haemophilus parainfluenzae ATCC 33392 | 651324039 | draft | img | 888828 |
66792 | Haemophilus parainfluenzae ATCC 33392 | 2916268294 | draft | img | 888828 |
66792 | Haemophilus parainfluenzae ATCC 33392 | GCA_000746485 | contig | ncbi | 888828 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 90.036 | yes |
flagellated | no | 94.669 | yes |
gram-positive | no | 98.107 | yes |
anaerobic | no | 95.018 | no |
aerobic | no | 93.018 | yes |
halophile | no | 61.981 | no |
spore-forming | no | 98.629 | no |
thermophile | no | 98.082 | yes |
glucose-util | yes | 50.518 | no |
glucose-ferment | yes | 54.722 | no |
External links
@ref: 3450
culture collection no.: DSM 8978, ATCC 33392, NCTC 7857, CCUG 12836, KCTC 15417, CIP 102513
straininfo link
- @ref: 80927
- straininfo: 40475
literature
Pubmed-ID | title | authors | journal | DOI | year | mesh | topic | topic2 |
---|---|---|---|---|---|---|---|---|
28674033 | Haemophilus parainfluenzae Strain ATCC 33392 Forms Biofilms In Vitro and during Experimental Otitis Media Infections. | Pang B, Swords WE | Infect Immun | 10.1128/IAI.01070-16 | 2017 | Animals, Antibiosis, Biofilms/*growth & development, Chinchilla, Disease Models, Animal, Haemophilus Infections/*microbiology/pathology, Haemophilus influenzae/growth & development/physiology, Haemophilus parainfluenzae/growth & development/*physiology, Otitis Media/*microbiology/pathology | ||
29381805 | A loop-mediated isothermal amplification procedure targeting the sodA gene for rapid and specific identification of Gallibacterium anatis. | Stepien-Pysniak D, Kosikowska U, Hauschild T, Burzynski A, Wilczynski J, Kolinska A, Nowaczek A, Marek A | Poult Sci | 10.3382/ps/pex420 | 2018 | Animals, Bacterial Proteins/*isolation & purification, *Chickens, Female, Nucleic Acid Amplification Techniques/methods/*veterinary, Pasteurellaceae/*isolation & purification, Pasteurellaceae Infections/diagnosis/microbiology/*veterinary, Poultry Diseases/*diagnosis/microbiology, Superoxide Dismutase/*isolation & purification, *Turkeys | Enzymology | Phylogeny |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3450 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8978) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8978 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
33555 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14208 | ||||
45838 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 12836) | https://www.ccug.se/strain?id=12836 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
68377 | Automatically annotated from API NH | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
80927 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40475.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121563 | Curators of the CIP | Collection of Institut Pasteur (CIP 102513) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102513 |