Strain identifier

BacDive ID: 11707

Type strain: Yes

Species: Haemophilus parainfluenzae

Strain Designation: Wound

Strain history: CIP <- 1986, NCTC <- 1949, A.B. Rosher, London, UK: strain Wound <- Glenson-White

NCBI tax ID(s): 888828 (strain), 729 (species)

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General

@ref: 3450

BacDive-ID: 11707

DSM-Number: 8978

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Haemophilus parainfluenzae Wound is a microaerophile, mesophilic bacterium that was isolated from septic finger.

NCBI tax id

NCBI tax idMatching level
729species
888828strain

strain history

@refhistory
3450<- ATCC; ATCC 33392 <- NCTC; NCTC 7857 <- A.B. Rosher;
67771<- CCUG <- R Mutters, Marburg, Germany <- NCTC <- AB Rosher, London, 1949 <- Glesson-White
121563CIP <- 1986, NCTC <- 1949, A.B. Rosher, London, UK: strain Wound <- Glenson-White

doi: 10.13145/bacdive11707.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Haemophilus
  • species: Haemophilus parainfluenzae
  • full scientific name: Haemophilus parainfluenzae Rivers 1922 (Approved Lists 1980)

@ref: 3450

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Haemophilus

species: Haemophilus parainfluenzae

full scientific name: Haemophilus parainfluenzae Rivers 1922

strain designation: Wound

type strain: yes

Morphology

cell morphology

@refmotilitygram staincell shapeconfidence
67771no
67771negative
121563nonegativerod-shaped
125439negative98.3
125438no93.768
125438negative97.082

colony morphology

@refincubation period
34501-2 days
121563

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3450HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
33555MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
121563CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperature
3450positivegrowth37
33555positivegrowth37
45838positivegrowth37
67771positivegrowth37
121563positivegrowth25-41
121563nogrowth15
121563nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
3450microaerophile
45838microaerophile
67771microaerophile
125439obligate aerobe98.7

spore formation

@refspore formationconfidence
125439no98.9
125438no91.939

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837727897tryptophan-energy source
6837717634D-glucose+builds acid from
1215634853esculin-hydrolysis
121563606565hippurate-hydrolysis
12156317632nitrate+reduction
12156316301nitrite-reduction

antibiotic resistance

  • @ref: 121563
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12156335581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
3450catalase+1.11.1.6
3450cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
121563oxidase-
121563beta-galactosidase+3.2.1.23
121563alcohol dehydrogenase-1.1.1.1
121563catalase-1.11.1.6
121563gamma-glutamyltransferase-2.3.2.2
121563lysine decarboxylase-4.1.1.18
121563ornithine decarboxylase+4.1.1.17
121563urease-3.5.1.5
68382alpha-glucosidase-3.2.1.20
68377tryptophan deaminase-4.1.99.1

fatty acid profile

  • @reffatty acidpercentageECL
    45838C14:018.514
    45838C16:029.716
    45838C18:00.618
    45838C13:0 ISO 2OH0.413.814
    45838C14:0 3OH/C16:1 ISO I7.415.485
    45838C14:1 CIS 7 DMA0.514.291
    45838C15:0 ISO 3OH0.716.135
    45838C16:1 ω7c37.415.819
    45838C16:1 ω7c DMA0.816.288
    45838C16:1 ω9c0.215.774
    45838C18:1 ω7c /12t/9t0.917.824
    45838C18:1 ω9c1.117.769
    45838C18:2 ω6,9c/C18:0 ANTE1.517.724
    45838unknown 14.5030.414.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    45838C14:02114
    45838C16:02616
    45838C18:00.418
    45838C13:0 ISO 2OH0.513.814
    45838C14:0 3OH/C16:1 ISO I9.815.485
    45838C16:1 ω7c39.515.819
    45838C18:1 ω9c1.117.769
    45838C18:2 ω6,9c/C18:0 ANTE1.317.724
    45838unknown 14.5030.514.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121563-+---+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3450-+++++--+----
3450+++++--++-+-
3450+++++-+/-++/----
3450+++++--++----
3450+++++--++-+--
3450-+------++---
3450+++++--++-+-
3450+++++-+/-++/----
3450-+++++--++---
3450-+++++--++-+-
3450++++++--++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3450septic finger
45838Human wound,septic fingerLondon?United KingdomGBREurope
67771From human wound, septic finger
121563Animal, Septic finger

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host Body-Site#Limb#Hand

taxonmaps

  • @ref: 69479
  • File name: preview.99_403.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_273;97_301;98_335;99_403&stattab=map
  • Last taxonomy: Haemophilus parainfluenzae subclade
  • 16S sequence: AY362908
  • Sequence Identity:
  • Total samples: 128855
  • soil counts: 1672
  • aquatic counts: 4078
  • animal counts: 121644
  • plant counts: 1461

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
34502Risk group (German classification)
1215632Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Haemophilus parainfluenzae strain DSM 8978 16S ribosomal RNA gene, partial sequenceHQ012020934nuccore888828
20218Haemophilus parainfluenzae ATCC 33392 16S ribosomal RNA gene, partial sequenceM750811483nuccore888828
3450Haemophilus parainfluenzae ATCC 33392 strain CCUG 12836 16S ribosomal RNA gene, partial sequenceAY3629081362nuccore888828
124043Haemophilus parainfluenzae strain CIP 102513 16S ribosomal RNA gene, partial sequence.EU0835301505nuccore888828
124043Haemophilus parainfluenzae CCUG 12836 gene for 16S rRNA, partial sequence.LC752345585nuccore888828
124043Haemophilus parainfluenzae CIP 102513 gene for 16S rRNA, partial sequence.LC752416585nuccore888828

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haemophilus parainfluenzae ATCC 33392GCA_000191405scaffoldncbi888828
66792Haemophilus parainfluenzae ATCC 33392 NCTC 7857GCA_900450845contigncbi888828
66792Haemophilus parainfluenzae ATCC 33392888828.3wgspatric888828
66792Haemophilus parainfluenzae ATCC 33392888828.6wgspatric888828
66792Haemophilus parainfluenzae ATCC 33392 strain NCTC 7857888828.150wgspatric888828
66792Haemophilus parainfluenzae strain FDAARGOS_1000729.2141completepatric729
66792Haemophilus parainfluenzae ATCC 33392651324039draftimg888828
66792Haemophilus parainfluenzae ATCC 333922916268294draftimg888828
66792Haemophilus parainfluenzae ATCC 33392GCA_000746485contigncbi888828

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno97.082yes
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no87.344yes
125438spore-formingspore-formingAbility to form endo- or exosporesno91.939no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no87.207yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno97yes
125438motile2+flagellatedAbility to perform flagellated movementno93.768no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno98.9
125439BacteriaNetmotilityAbility to perform movementno55.5
125439BacteriaNetgram_stainReaction to gram-stainingnegative98.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe98.7

External links

@ref: 3450

culture collection no.: DSM 8978, ATCC 33392, NCTC 7857, CCUG 12836, KCTC 15417, CIP 102513

straininfo link

  • @ref: 80927
  • straininfo: 40475

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
28674033Haemophilus parainfluenzae Strain ATCC 33392 Forms Biofilms In Vitro and during Experimental Otitis Media Infections.Pang B, Swords WEInfect Immun10.1128/IAI.01070-162017Animals, Antibiosis, Biofilms/*growth & development, Chinchilla, Disease Models, Animal, Haemophilus Infections/*microbiology/pathology, Haemophilus influenzae/growth & development/physiology, Haemophilus parainfluenzae/growth & development/*physiology, Otitis Media/*microbiology/pathology
29381805A loop-mediated isothermal amplification procedure targeting the sodA gene for rapid and specific identification of Gallibacterium anatis.Stepien-Pysniak D, Kosikowska U, Hauschild T, Burzynski A, Wilczynski J, Kolinska A, Nowaczek A, Marek APoult Sci10.3382/ps/pex4202018Animals, Bacterial Proteins/*isolation & purification, *Chickens, Female, Nucleic Acid Amplification Techniques/methods/*veterinary, Pasteurellaceae/*isolation & purification, Pasteurellaceae Infections/diagnosis/microbiology/*veterinary, Poultry Diseases/*diagnosis/microbiology, Superoxide Dismutase/*isolation & purification, *TurkeysEnzymologyPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3450Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8978)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8978
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33555Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14208
45838Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 12836)https://www.ccug.se/strain?id=12836
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80927Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40475.1StrainInfo: A central database for resolving microbial strain identifiers
121563Curators of the CIPCollection of Institut Pasteur (CIP 102513)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102513
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1