Strain identifier

BacDive ID: 11698

Type strain: Yes

Species: Haemophilus influenzae

Strain Designation: 680

Strain history: CIP <- 1986, NCTC <- 1950, A.B. Rosher, London, UK <- M. Pittman, USA: strain 680

NCBI tax ID(s): 727 (species)

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General

@ref: 1685

BacDive-ID: 11698

DSM-Number: 4690

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Haemophilus influenzae 680 is a microaerophile, mesophilic, Gram-negative bacterium of the family Pasteurellaceae.

NCBI tax id

  • NCBI tax id: 727
  • Matching level: species

strain history

@refhistory
1685<- NCTC; NCTC 8143 <- A.B. Rosher; <- M. Pittman, NIH; 680
67770CIP 102514 <-- NCTC 8143 <-- A.B. Rosher; UK <-- M. Pittman; NIH, USA; 680.
120105CIP <- 1986, NCTC <- 1950, A.B. Rosher, London, UK <- M. Pittman, USA: strain 680

doi: 10.13145/bacdive11698.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Haemophilus
  • species: Haemophilus influenzae
  • full scientific name: Haemophilus influenzae corrig. (Lehmann and Neumann 1896) Winslow et al. 1917 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Hemophilus influenzae
    20215Bacterium influenzae

@ref: 1685

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Haemophilus

species: Haemophilus influenzae

full scientific name: Haemophilus influenzae (Lehmann and Neumann 1896) Winslow et al. 1917

strain designation: 680

type strain: yes

Morphology

cell morphology

  • @ref: 120105
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation period
16851-2 days
120105

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1685HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
37092MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
1685COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
120105CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
1685positivegrowth37mesophilic
37092positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
1685microaerophile
120105facultative anaerobe

antibiogram

@refmediumPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactamincubation temperatureincubation timeoxygen condition
1685M 80430163440-4242-443430-32505034-36444624-2630818-20124642-442230401022-2444-462240>5018-20>504218-200221050
1685M 80428030-324036-383026-28424030344022-242281810383818223801826-2818304216>5036-3820-2210-1220840371aerob
1685Mueller-Hinton Agar2803040-42363430-324440-4232-34384022-2422-24816-1803838202440022-242620324416503412022-24836-38
1685Mueller-Hinton Agar30-3203442-4438-4030-32285042-443240-4244-46262882010-124242202842022302036502022-244024020640-42371microaerophil
1685M 804328-103242-4440-4234304442-4428-3036-384222248181040-42402022-244002228-302036-38n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.37
1685M 80426233034-36402424484028-3038-404020-22246-820638-403818243412-14182618-2032-3446-4820-22>503820-2202864037microaerophil

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120105nitrate+reduction17632
120105nitrite-reduction16301

antibiotic resistance

  • @ref: 120105
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120105
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
1685catalase+1.11.1.6
1685cytochrome-c oxidase+1.9.3.1
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
120105oxidase-
120105beta-galactosidase-3.2.1.23
120105alcohol dehydrogenase-1.1.1.1
120105catalase+1.11.1.6
120105lysine decarboxylase-4.1.1.18
120105ornithine decarboxylase-4.1.1.17
120105urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120105-+++-+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120105----++---++----------+-----+--------------------+/-

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
1685-++---+-+--++
1685++---+-+---+-
1685++-+/--+-+---+-
1685++/----+-+---+-
1685++---+-+---+-
1685+++/-+/--+-+---+-
1685-++---+-+--++
1685-++---+-+---+
1685-++-+/--+-+---+
1685-++/----+-+---+
1685-+++/-+/--+-+---+
1685-++---+-+---+
1685-+----+-+---+

Isolation, sampling and environmental information

isolation

  • @ref: 1685
  • country: USA
  • origin.country: USA
  • continent: North America

taxonmaps

  • @ref: 69479
  • File name: preview.99_36.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_30;97_31;98_34;99_36&stattab=map
  • Last taxonomy: Haemophilus influenzae
  • 16S sequence: M35019
  • Sequence Identity:
  • Total samples: 31536
  • soil counts: 173
  • aquatic counts: 597
  • animal counts: 30711
  • plant counts: 55

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
16852Risk group (German classification)
1201052Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218H.influenzae 16S rRNA, V3-V4-V5 variable regions, segment 1M23300100ena727
20218H.influenzae 16S rRNA, V3-V4-V5 variable regions, segment 2M23301345ena727
20218Haemophilus influenzae ATCC 33391 16S rRNA gene, partial sequenceU02897391ena727
1685Haemophilus influenzae strain ATCC 33391 16S ribosomal RNA gene, partial sequenceM350191486ena727

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haemophilus influenzae NCTC8143GCA_001457655completencbi727
66792Haemophilus influenzae strain NCTC8143727.583completepatric727
66792Haemophilus influenzae NCTC81432660238341completeimg727
66792Haemophilus influenzae strain FDAARGOS_1560727.3050completepatric727
66792Haemophilus influenzae strain FDAARGOS_1560727.3041completepatric727

GC content

@refGC-contentmethod
168538.9thermal denaturation, midpoint method (Tm)
6777039thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.095no
gram-positiveno98.121no
anaerobicno95.317yes
aerobicno95.08yes
halophileyes50.62no
spore-formingno98.371no
glucose-utilno53.562no
motileno91.432no
thermophileno98.134no
glucose-fermentyes52.011no

External links

@ref: 1685

culture collection no.: DSM 4690, ATCC 33391, NCTC 8143, JCM 33542, CCUG 23945, CIP 102514

straininfo link

  • @ref: 80918
  • straininfo: 40474

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology9298647Identifying the major proteome components of Haemophilus influenzae type-strain NCTC 8143.Link AJ, Hays LG, Carmack EB, Yates JR 3rdElectrophoresis10.1002/elps.11501808081997Bacterial Proteins/*genetics/*isolation & purification, Electrophoresis, Gel, Two-Dimensional/*methods, *Genome, Bacterial, Haemophilus influenzae/*chemistry/*genetics, Isoelectric Point, Mass Spectrometry/methods, Molecular Weight, Peptide Mapping/*methodsGenetics
Metabolism12591874Exogenous glutathione completes the defense against oxidative stress in Haemophilus influenzae.Vergauwen B, Pauwels F, Vaneechoutte M, Van Beeumen JJJ Bacteriol10.1128/JB.185.5.1572-1581.20032003Antioxidants/metabolism, Catalase/drug effects/genetics/metabolism, Cloning, Molecular, Drug Resistance, Microbial/genetics, Escherichia coli/drug effects/genetics, Genetic Complementation Test, Glutathione/*analogs & derivatives/*metabolism/pharmacology, Glutathione Peroxidase/drug effects/genetics/metabolism, Haemophilus influenzae/drug effects/*physiology, Inactivation, Metabolic, Mutation, *Oxidative Stress, Phagocytosis/drug effects, Pyruvaldehyde/pharmacology, S-Nitrosoglutathione/pharmacology, Sulfur/metabolism, tert-Butylhydroperoxide/pharmacologyPathogenicity
Metabolism15047524In vitro activities of piperacillin against beta-lactamase-negative ampicillin-resistant Haemophilus influenzae.Morikawa Y, Kitazato M, Mitsuyama J, Mizunaga S, Minami S, Watanabe YAntimicrob Agents Chemother10.1128/AAC.48.4.1229-1234.20042004Ampicillin Resistance/*genetics, Bacterial Proteins/metabolism, Carrier Proteins/metabolism, Cefotaxime/pharmacology, Ceftriaxone/pharmacology, Cephalosporins/pharmacology, Haemophilus influenzae/*drug effects/enzymology/genetics, Hexosyltransferases/metabolism, Humans, Kinetics, Meningitis, Haemophilus/microbiology, Microbial Sensitivity Tests, Muramoylpentapeptide Carboxypeptidase/metabolism, Penicillin-Binding Proteins, Penicillins/*pharmacology, Peptidyl Transferases/metabolism, Piperacillin/*pharmacology, beta-Lactamases/*geneticsEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1685Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4690)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4690
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37092Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14209
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80918Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40474.1StrainInfo: A central database for resolving microbial strain identifiers
120105Curators of the CIPCollection of Institut Pasteur (CIP 102514)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102514