Strain identifier

BacDive ID: 11691

Type strain: Yes

Species: Actinobacillus anseriformium

Strain Designation: F66

Strain history: <- CCM <- H. Christensen; F66

NCBI tax ID(s): 221404 (species)

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General

@ref: 18213

BacDive-ID: 11691

DSM-Number: 26423

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, ovoid-shaped, animal pathogen

description: Actinobacillus anseriformium F66 is a microaerophile, mesophilic, Gram-negative animal pathogen that was isolated from conjunctivitis in a White Pekin duck.

NCBI tax id

  • NCBI tax id: 221404
  • Matching level: species

strain history

  • @ref: 18213
  • history: <- CCM <- H. Christensen; F66

doi: 10.13145/bacdive11691.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus anseriformium
  • full scientific name: Actinobacillus anseriformium Bisgaard and Christensen 2012

@ref: 18213

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus anseriformium

full scientific name: Actinobacillus anseriformium Bisgaard and Christensen 2012

strain designation: F66

type strain: yes

Morphology

cell morphology

  • @ref: 30043
  • gram stain: negative
  • cell shape: ovoid-shaped
  • motility: no

colony morphology

  • @ref: 18213
  • type of hemolysis: beta
  • hemolysis ability: 1
  • incubation period: 1-2 days

pigmentation

  • @ref: 30043
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 18213
  • name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/693
  • composition: Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
18213positivegrowth37mesophilic
30043positivegrowth22-37
62277positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62277
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3004317057cellobiose+carbon source
3004337684mannose+carbon source
3004327082trehalose+carbon source
6837717634D-glucose+builds acid from
6837715824D-fructose+builds acid from
6837727897tryptophan-energy source

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
18213catalase+1.11.1.6
18213cytochrome-c oxidase+1.9.3.1
30043alpha-galactosidase+3.2.1.22
30043catalase+1.11.1.6
30043cytochrome oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
62277-+---+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
18213++++-+-+-----
62277-++-+-+-+----
18213-++++-+-+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
18213conjunctivitis in a White Pekin duckDenmarkDNKEurope
62277White Pekin duck conjunctivitisDenmarkDNKEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host#Birds
#Host Body-Site#Organ#Eye

taxonmaps

  • @ref: 69479
  • File name: preview.99_41218.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_155;97_23752;98_30008;99_41218&stattab=map
  • Last taxonomy: Actinobacillus anseriformium subclade
  • 16S sequence: AY172727
  • Sequence Identity:
  • Total samples: 782
  • soil counts: 13
  • aquatic counts: 31
  • animal counts: 737
  • plant counts: 1

Safety information

risk assessment

  • @ref: 18213
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18213
  • description: Actinobacillus anseriformium biovar 1 strain F66 16S ribosomal RNA gene, partial sequence
  • accession: AY172727
  • length: 1450
  • database: ena
  • NCBI tax ID: 221398

External links

@ref: 18213

culture collection no.: DSM 26423, CCM 7846, CCUG 60324

straininfo link

  • @ref: 80911
  • straininfo: 404299

literature

  • topic: Phylogeny
  • Pubmed-ID: 21441378
  • title: Classification of avian haemolytic Actinobacillus-like organisms (Bisgaard taxon 26) associated with anseriforme birds as Actinobacillus anseriformium sp. nov.
  • authors: Bisgaard M, Christensen H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.028746-0
  • year: 2011
  • mesh: Actinobacillus/*classification/genetics/*isolation & purification, Actinobacillus Infections/microbiology/*veterinary, Animals, Anseriformes/*microbiology, Bacterial Proteins/genetics, Bird Diseases/*microbiology, Cattle, DNA Restriction Enzymes/genetics, DNA, Bacterial/analysis/genetics, DNA-Directed RNA Polymerases/genetics, Genotype, Hemolysis, Molecular Sequence Data, Pasteurellaceae/classification/genetics/isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, Respiratory Tract Infections/microbiology/*veterinary, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18213Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26423)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26423
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30043Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2640428776041
62277Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 60324)https://www.ccug.se/strain?id=60324
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80911Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404299.1StrainInfo: A central database for resolving microbial strain identifiers