Strain identifier

BacDive ID: 11582

Type strain: Yes

Species: Paenibacillus cineris

Strain Designation: B1768

Strain history: CIP <- 2003, N.A. Logan, Caledonian Univ., Glasgow, UK: strain B1768

NCBI tax ID(s): 237530 (species)

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General

@ref: 6662

BacDive-ID: 11582

DSM-Number: 16945

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Paenibacillus cineris B1768 is an aerobe, spore-forming, mesophilic bacterium that was isolated from volcanic ash.

NCBI tax id

  • NCBI tax id: 237530
  • Matching level: species

strain history

@refhistory
6662<- CIP
366892003, N.A. Logan, Caledonian Univ., Glasgow, UK: strain B1768
118287CIP <- 2003, N.A. Logan, Caledonian Univ., Glasgow, UK: strain B1768

doi: 10.13145/bacdive11582.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus cineris
  • full scientific name: Paenibacillus cineris Logan et al. 2004

@ref: 6662

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus cineris

full scientific name: Paenibacillus cineris Logan et al. 2004

strain designation: B1768

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30123negative3.25 µm0.8 µmrod-shapedyes
118287positiverod-shapedyes

colony morphology

  • @ref: 118287

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6662CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 Distilled water
36689MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118287CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6662positivegrowth30mesophilic
30123positivegrowth8.0-50
30123positiveoptimum29mesophilic
36689positivegrowth30mesophilic
118287positivegrowth22-45
118287nogrowth10psychrophilic
118287nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
30123positivegrowth5.0-11.0alkaliphile
30123positiveoptimum7
118287positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30123aerobe
118287facultative anaerobe

spore formation

  • @ref: 30123
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
30123NaClpositivegrowth3.0-5.0 %
30123NaClpositiveoptimum4 %
118287NaClpositivegrowth0-4 %
118287NaClnogrowth6 %
118287NaClnogrowth8 %
118287NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3012322599arabinose+carbon source
3012317057cellobiose+carbon source
3012328757fructose+carbon source
3012328260galactose+carbon source
3012317234glucose+carbon source
3012328087glycogen+carbon source
3012317716lactose+carbon source
3012317306maltose+carbon source
3012329864mannitol+carbon source
3012337684mannose+carbon source
3012328053melibiose+carbon source
30123506227N-acetylglucosamine+carbon source
3012316634raffinose+carbon source
3012330911sorbitol+carbon source
3012318222xylose+carbon source
301234853esculin+hydrolysis
3012317632nitrate+reduction
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch+builds acid from
6837116634raffinose+builds acid from
6837115443inulin-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside+builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11828716947citrate-carbon source
1182874853esculin+hydrolysis
118287606565hippurate+hydrolysis
11828717632nitrate+reduction
11828716301nitrite-reduction
11828717632nitrate+respiration

antibiotic resistance

  • @ref: 118287
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 118287
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11828715688acetoin-
11828717234glucose-

enzymes

@refvalueactivityec
30123cytochrome oxidase+1.9.3.1
118287oxidase+
118287beta-galactosidase+3.2.1.23
118287alcohol dehydrogenase-1.1.1.1
118287gelatinase+/-
118287amylase-
118287DNase+
118287caseinase-3.4.21.50
118287catalase+1.11.1.6
118287tween esterase-
118287gamma-glutamyltransferase-2.3.2.2
118287lecithinase+
118287lipase-
118287lysine decarboxylase-4.1.1.18
118287ornithine decarboxylase-4.1.1.17
118287protease+
118287tryptophan deaminase-
118287urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118287--++-+---+--++-----+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118287---+++--++++/-+/--+/--+/-+---+++++++++++/--+/-++--++------+/--+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118287++++--+++++++++++++++++--+------++++-+----------------+-+--++--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6662volcanic ashCandlemas IslandUnited KingdomGBREurope
118287Volcanic ashCandlemas IslandUnited KingdomGBREurope1998

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Dust
#Environmental#Terrestrial#Volcanic

taxonmaps

  • @ref: 69479
  • File name: preview.99_5101.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_2482;97_3032;98_3807;99_5101&stattab=map
  • Last taxonomy: Paenibacillus
  • 16S sequence: AJ575658
  • Sequence Identity:
  • Total samples: 3736
  • soil counts: 2631
  • aquatic counts: 282
  • animal counts: 403
  • plant counts: 420

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
66621Risk group (German classification)
1182871Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6662
  • description: Paenibacillus cineris partial 16S rRNA gene, type strain LMG 18439T
  • accession: AJ575658
  • length: 1505
  • database: ena
  • NCBI tax ID: 237530

GC content

  • @ref: 6662
  • GC-content: 51.5

External links

@ref: 6662

culture collection no.: DSM 16945, CIP 108109, LMG 18439, SSI 068, KCTC 3998

straininfo link

  • @ref: 80810
  • straininfo: 12028

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15280271Paenibacillus cineris sp. nov. and Paenibacillus cookii sp. nov., from Antarctic volcanic soils and a gelatin-processing plant.Logan NA, De Clerck E, Lebbe L, Verhelst A, Goris J, Forsyth G, Rodriguez-Diaz M, Heyndrickx M, De Vos PInt J Syst Evol Microbiol10.1099/ijs.0.02967-02004Aerobiosis, Antarctic Regions, Bacterial Proteins/analysis/isolation & purification, Bacterial Typing Techniques, DNA Fingerprinting, DNA, Bacterial/analysis/chemistry/isolation & purification, DNA, Ribosomal/analysis/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, *Gelatin, Genes, rRNA/genetics, Gram-Positive Endospore-Forming Bacteria/*classification/cytology/isolation & purification/physiology, *Industrial Microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, Proteome/analysis/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, *Soil MicrobiologyEnzymology
Phylogeny15879272Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum.Rivas R, Gutierrez C, Abril A, Mateos PF, Martinez-Molina E, Ventosa A, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.63513-02005Aerobiosis, Argentina, Base Composition, Carbohydrate Metabolism, Catalase/analysis, Cicer/*microbiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Esculin/metabolism, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Gram-Positive Endospore-Forming Rods/*classification/cytology/*isolation & purification/physiology, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Oxidoreductases/analysis, Phenazines, Phylogeny, Plant Roots/*microbiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial, beta-Glucosidase/analysisEnzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6662Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16945)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16945
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30123Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2647728776041
36689Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5695
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80810Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12028.1StrainInfo: A central database for resolving microbial strain identifiers
118287Curators of the CIPCollection of Institut Pasteur (CIP 108109)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108109