Strain identifier

BacDive ID: 11558

Type strain: Yes

Species: Paenibacillus chinjuensis

Strain Designation: WN9

Strain history: CIP <- 2002, JCM <- 2000, YH Park: strain WN9

NCBI tax ID(s): 103815 (species)

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General

@ref: 5683

BacDive-ID: 11558

DSM-Number: 15045

keywords: 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Paenibacillus chinjuensis WN9 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 103815
  • Matching level: species

strain history

@refhistory
5683<- J.-H. Yoon; WN9
67770Y.-H. Park WN9.
118233CIP <- 2002, JCM <- 2000, YH Park: strain WN9

doi: 10.13145/bacdive11558.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus chinjuensis
  • full scientific name: Paenibacillus chinjuensis Yoon et al. 2002

@ref: 5683

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus chinjuensis

full scientific name: Paenibacillus chinjuensis Yoon et al. 2002

strain designation: WN9

type strain: yes

Morphology

cell morphology

  • @ref: 118233
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118233

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5683NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
33795MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118233CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5683positivegrowth30mesophilic
33795positivegrowth30mesophilic
67770positivegrowth30mesophilic
118233positivegrowth22-45
118233nogrowth10psychrophilic
118233nogrowth55thermophilic

culture pH

  • @ref: 118233
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 118233
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 118233
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
118233NaClpositivegrowth0-2 %
118233NaClnogrowth4 %
118233NaClnogrowth6 %
118233NaClnogrowth8 %
118233NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118233citrate-carbon source16947
118233esculin-hydrolysis4853
118233hippurate+hydrolysis606565
118233nitrate-reduction17632
118233nitrite-reduction16301
118233nitrate-respiration17632

metabolite production

@refChebi-IDmetaboliteproduction
6777072813exopolysaccharideyes
11823335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11823315688acetoin-
11823317234glucose+

enzymes

@refvalueactivityec
118233oxidase+
118233beta-galactosidase-3.2.1.23
118233alcohol dehydrogenase-1.1.1.1
118233gelatinase+
118233amylase+
118233DNase-
118233caseinase+3.4.21.50
118233catalase+1.11.1.6
118233tween esterase+
118233gamma-glutamyltransferase-2.3.2.2
118233lecithinase-
118233lipase-
118233lysine decarboxylase-4.1.1.18
118233ornithine decarboxylase-4.1.1.17
118233protease+
118233urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118233-+++-+----++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118233----+/--------------------+/--+/----+/-+/----+/-+/--+/-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118233+--++--+-++----++--+--+-----------++-----+-----------------++-------------------------+---++++-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5683soilKoreaRepublic of KoreaKORAsia
67770SoilChinjuRepublic of KoreaKORAsia
118233Environment, SoilChinjuRepublic of KoreaKORAsia1998

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56831Risk group (German classification)
1182331Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Paenibacillus sp. WN9 16S ribosomal RNA gene, partial sequence
  • accession: AF164345
  • length: 1488
  • database: ena
  • NCBI tax ID: 103815

GC content

  • @ref: 67770
  • GC-content: 53
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5683

culture collection no.: DSM 15045, JCM 10939, KCTC 8951, BCRC 17344, CIP 107433, KCTC 8951P

straininfo link

  • @ref: 80786
  • straininfo: 97893

literature

  • topic: Phylogeny
  • Pubmed-ID: 11931150
  • title: Paenibacillus chinjuensis sp. nov., a novel exopolysaccharide-producing bacterium.
  • authors: Yoon JH, Seo WT, Shin YK, Kho YH, Kang KH, Park YH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-2-415
  • year: 2002
  • mesh: Anaerobiosis, Bacillus/classification/metabolism, DNA, Bacterial/chemistry, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Gram-Positive Endospore-Forming Rods/*classification/isolation & purification/metabolism, Korea, Molecular Sequence Data, Peptidoglycan/chemistry, Phylogeny, Polysaccharides, Bacterial/biosynthesis/chemistry, RNA, Ribosomal/chemistry, RNA, Ribosomal, 16S/chemistry, Sequence Homology, Nucleic Acid, Vitamin K 2/analysis
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5683Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15045)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15045
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33795Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4868
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
80786Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID97893.1StrainInfo: A central database for resolving microbial strain identifiers
118233Curators of the CIPCollection of Institut Pasteur (CIP 107433)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107433