Strain identifier

BacDive ID: 11515

Type strain: Yes

Species: Paenibacillus apiarius

Strain Designation: BX 3

Strain history: CIP <- 1996, L.K. Nakamura, USDA, Peoria, IL, USA: strain NRRL NRS-1438 <- R.E. Gordon <- 1953, N.R. Smith <- H. Katznelson

NCBI tax ID(s): 46240 (species)

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General

@ref: 2231

BacDive-ID: 11515

DSM-Number: 5581

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Paenibacillus apiarius BX 3 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from bee larvae.

NCBI tax id

  • NCBI tax id: 46240
  • Matching level: species

strain history

@refhistory
2231<- NRRL <- H. Katznelson, Canadian Dept. of Agr., (Bacillus apiarius) BX 3
116421CIP <- 1996, L.K. Nakamura, USDA, Peoria, IL, USA: strain NRRL NRS-1438 <- R.E. Gordon <- 1953, N.R. Smith <- H. Katznelson

doi: 10.13145/bacdive11515.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus apiarius
  • full scientific name: Paenibacillus apiarius (ex Katznelson 1955) Nakamura 1996

@ref: 2231

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus apiarius

full scientific name: Paenibacillus apiarius (ex Katznelson 1955) Nakamura 1996

strain designation: BX 3

type strain: yes

Morphology

cell morphology

  • @ref: 116421
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 116421
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2231NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39136MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116421CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
2231positivegrowth30mesophilic
39136positivegrowth30mesophilic
116421positivegrowth22-37
116421nogrowth10psychrophilic
116421nogrowth45thermophilic
116421nogrowth55thermophilic

culture pH

  • @ref: 116421
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 116421
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 116421
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
116421NaClpositivegrowth0-2 %
116421NaClnogrowth4 %
116421NaClnogrowth6 %
116421NaClnogrowth8 %
116421NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
116421citrate-carbon source16947
116421esculin+hydrolysis4853
116421nitrate+reduction17632
116421nitrite-reduction16301
116421nitrate+respiration17632

metabolite production

  • @ref: 116421
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11642115688acetoin-
11642117234glucose-

enzymes

@refvalueactivityec
116421oxidase-
116421beta-galactosidase+3.2.1.23
116421alcohol dehydrogenase-1.1.1.1
116421gelatinase-
116421amylase-
116421DNase-
116421caseinase-3.4.21.50
116421catalase+1.11.1.6
116421tween esterase-
116421gamma-glutamyltransferase-2.3.2.2
116421lecithinase-
116421lipase-
116421lysine decarboxylase-4.1.1.18
116421ornithine decarboxylase-4.1.1.17
116421urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116421---+---------+--+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116421+---+----++-----+---++++++++-+++--+++-++------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116421+-++--+++++----+++++--+--------++-++-----+--------++-+-----++---------------------+-------+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2231bee larvae
116421Animal, Honeybee larvaOttawaCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host#Juvenile

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
22311Risk group (German classification)
1164211Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Paenibacillus apiarius gene for 16S rRNA, partial sequenceAB0732011531ena46240
20218Paenibacillus apiarius partial 16S rRNA gene, strain DSM 5581TAJ3204921489ena46240
20218Paenibacillus apiarius 16S ribosomal RNA geneU492471489ena46240

External links

@ref: 2231

culture collection no.: DSM 5581, ATCC 29575, NCIMB 13506, NRRL NRS-1438, CIP 104902

straininfo link

  • @ref: 80747
  • straininfo: 9978

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8782677Paenibacillus apiarius sp. nov.Nakamura LKInt J Syst Bacteriol10.1099/00207713-46-3-6881996Bacillus/*classification/genetics/metabolism/ultrastructure, Base Sequence, DNA, Bacterial, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16SGenetics
Phylogeny23408056Paenibacillus profundus sp. nov., a deep sediment bacterium that produces isocoumarin and peptide antibiotics.Romanenko LA, Tanaka N, Svetashev VI, Kalinovskaya NIArch Microbiol10.1007/s00203-013-0873-y2013Anti-Bacterial Agents/*biosynthesis, DNA, Bacterial/genetics, Isocoumarins/*metabolism, Paenibacillus/classification/cytology/*isolation & purification/*physiology, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyMetabolism
Phylogeny28555445Paenibacillus aquistagni sp. nov., isolated from an artificial lake accumulating industrial wastewater.Simon L, Skraban J, Kyrpides NC, Woyke T, Shapiro N, Cleenwerck I, Vandamme P, Whitman WB, Trcek JAntonie Van Leeuwenhoek10.1007/s10482-017-0891-x2017Base Composition, Carbohydrate Metabolism, Cell Wall/chemistry, Enzymes/metabolism, Fimbriae, Bacterial/genetics, Genome Size, Genome, Bacterial, Lakes/microbiology, Nucleic Acid Hybridization, Paenibacillus/chemistry/*classification/physiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Slovenia, Species Specificity, Sugars/metabolism, Waste Water/*microbiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2231Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5581)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5581
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39136Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16861
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
80747Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9978.1StrainInfo: A central database for resolving microbial strain identifiers
116421Curators of the CIPCollection of Institut Pasteur (CIP 104902)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104902