General

@ref: 3876

BacDive-ID: 1148

DSM-Number: 10277

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Bacillus infernus TH-23 is an anaerobe, thermophilic bacterium that was isolated from core sample from 2.7 km depth.

NCBI tax id

• NCBI tax id: 38072
• Matching level: species

strain history

• @ref: 3876
• history: <- OCM <- D. Boone, TH-23

doi: 10.13145/bacdive1148.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Bacilli
• order: Caryophanales
• family: Bacillaceae
• genus: Bacillus
• species: Bacillus infernus
• full scientific name: Bacillus infernus Boone et al. 1995

@ref: 3876

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus infernus

full scientific name: Bacillus infernus Boone et al. 1995

strain designation: TH-23

type strain: yes

Culture and growth conditions

culture medium

• @ref: 3876
• name: BACILLUS INFERNUS MEDIUM (DSMZ Medium 705)
• growth: yes
• link: https://mediadive.dsmz.de/medium/705
• composition: Name: BACILLUS INFERNUS MEDIUM (DSMZ Medium 705) Composition: Fe(III)-citrate x 5 H2O 6.7 g/l NaCl 5.8 g/l Yeast extract 2.0 g/l Trypticase peptone 2.0 g/l Na-lactate 1.2 g/l NH4Cl 1.0 g/l MgCl2 x 6 H2O 1.0 g/l Na-acetate 0.8 g/l 2-Mercaptoethanesulfonic acid 0.5 g/l K2HPO4 x 3 H2O 0.4 g/l Na2-EDTA x 2 H2O 0.005 g/l CoCl2 x 6 H2O 0.0015 g/l ZnCl2 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l AlCl3 x 6 H2O 0.0004 g/l Na2WO4 x 2 H2O 0.0004 g/l Na2SeO3 x 5 H2O 0.0003 g/l CuCl2 x 2 H2O 0.0002 g/l NiCl2 x 6 H2O 0.0002 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l NaOH Distilled water

culture temp

• @ref: 3876
• growth: positive
• type: growth
• temperature: 55
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 3876
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 3876
• sample type: core sample from 2.7 km depth
• geographic location: Virginia, Taylorsville Triassic Basin
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

• Cat1: #Environmental
• Cat2: #Terrestrial
• Cat3: #Core sample

taxonmaps

• @ref: 69479
• File name: preview.99_28945.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_12928;97_15694;98_21589;99_28945&stattab=map
• Last taxonomy: Bacillus infernus subclade
• 16S sequence: U20385
• Sequence Identity:
• Total samples: 3541
• soil counts: 2255
• aquatic counts: 476
• animal counts: 518
• plant counts: 292

Safety information

risk assessment

• @ref: 3876
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 3876
• description: Bacillus infernus strain TH-23 16S small subunit ribosomal RNA gene, partial sequence
• accession: U20385
• length: 1508
• database: ena
• NCBI tax ID: 38072

External links

@ref: 3876

culture collection no.: DSM 10277, OCM 479

straininfo link

• @ref: 70819
• straininfo: 92592

literature

• topic: Phylogeny
• Pubmed-ID: 8590670
• title: Bacillus infernus sp. nov., an Fe(III)- and Mn(IV)-reducing anaerobe from the deep terrestrial subsurface.
• authors: Boone DR, Liu Y, Zhao ZJ, Balkwill DL, Drake GR, Stevens TO, Aldrich HC
• journal: Int J Syst Bacteriol
• DOI: 10.1099/00207713-45-3-441
• year: 1995
• mesh: Anaerobiosis, Bacillus/*classification/genetics/metabolism, Base Sequence, Carbohydrate Metabolism, Chlorides, DNA, Ribosomal/chemistry, *Environmental Microbiology, Ferric Compounds/metabolism, Hydrogen-Ion Concentration, Manganese Compounds/metabolism, Molecular Sequence Data, Oxidation-Reduction, Oxides/metabolism, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/chemistry, Sequence Homology, Nucleic Acid, Sodium Chloride/pharmacology, Temperature, Time Factors
• topic2: Metabolism

Reference