Strain identifier

BacDive ID: 11440

Type strain: Yes

Species: Brevibacillus fluminis

Strain Designation: CJ71

Strain history: C.-J. Cha CJ71.

NCBI tax ID(s): 511487 (species)

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General

@ref: 17357

BacDive-ID: 11440

DSM-Number: 23904

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile

description: Brevibacillus fluminis CJ71 is an aerobe, spore-forming, mesophilic bacterium that was isolated from sediment of estuarine wetland.

NCBI tax id

  • NCBI tax id: 511487
  • Matching level: species

strain history

@refhistory
17357<- JCM <- C.-J. Cha, Chung-Ang Univ., Seoul, Republic of Korea; CJ71 <- KACC
67770C.-J. Cha CJ71.

doi: 10.13145/bacdive11440.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Brevibacillus
  • species: Brevibacillus fluminis
  • full scientific name: Brevibacillus fluminis Choi et al. 2010

@ref: 17357

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Brevibacillus

species: Brevibacillus fluminis

full scientific name: Brevibacillus fluminis Choi et al. 2010

strain designation: CJ71

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
29400positiveyes
69480yes92.817
69480positive100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17357TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)yeshttps://mediadive.dsmz.de/medium/545Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
17357CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17357positivegrowth30mesophilic
29400positivegrowth20-45
29400positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
29400positivegrowth05-08
29400positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 29400
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
29400yes
69481yes100
69480yes100

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2940017057cellobiose+carbon source
2940028757fructose+carbon source
2940033984fucose+carbon source
2940028260galactose+carbon source
2940028087glycogen+carbon source
2940017306maltose+carbon source
2940029864mannitol+carbon source
29400506227N-acetylglucosamine+carbon source
2940017814salicin+carbon source
2940027082trehalose+carbon source
2940017151xylitol+carbon source

enzymes

@refvalueactivityec
29400acid phosphatase+3.1.3.2
29400catalase+1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17357sediment of estuarine wetlandHan RiverRepublic of KoreaKORAsia
67770Sediment of the estuarine wetland of the Han RiverRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Estuary
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Wetland (Swamp)

taxonmaps

  • @ref: 69479
  • File name: preview.99_10318.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_95;96_577;97_5647;98_7693;99_10318&stattab=map
  • Last taxonomy: Brevibacillus fluminis subclade
  • 16S sequence: EU375457
  • Sequence Identity:
  • Total samples: 2029
  • soil counts: 1239
  • aquatic counts: 345
  • animal counts: 257
  • plant counts: 188

Safety information

risk assessment

  • @ref: 17357
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17357
  • description: Brevibacillus fluminis strain CJ71 16S ribosomal RNA gene, partial sequence
  • accession: EU375457
  • length: 1413
  • database: ena
  • NCBI tax ID: 511487

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacillus fluminis strain JCM 15716511487.5wgspatric511487
66792Brevibacillus fluminis JCM 157162851217762draftimg511487
67770Brevibacillus fluminis JCM 15716GCA_003710825contigncbi511487

GC content

@refGC-contentmethod
1735752.4high performance liquid chromatography (HPLC)
2940052.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes84.568yes
anaerobicno95.895no
halophileno95.793no
spore-formingyes95.183no
glucose-utilyes87.574no
aerobicyes78.052no
thermophileno99.531no
motileyes90.71yes
flagellatedyes56.397no
glucose-fermentno90.903no

External links

@ref: 17357

culture collection no.: DSM 23904, JCM 15716, KACC 13381

straininfo link

  • @ref: 80678
  • straininfo: 404432

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19700454Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland.Choi MJ, Bae JY, Kim KY, Kang H, Cha CJInt J Syst Evol Microbiol10.1099/ijs.0.012351-02009Base Sequence, Fatty Acids/analysis, Geologic Sediments/*microbiology, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, *WetlandsGenetics
Phylogeny27902214Brevibacillus halotolerans sp. nov., isolated from saline soil of a paddy field.Song J, Wang Y, Song Y, Zhao B, Wang H, Zhou S, Kong D, Guo X, Li Y, He M, Ma K, Ruan Z, Yan YInt J Syst Evol Microbiol10.1099/ijsem.0.0015792017Bacterial Typing Techniques, Base Composition, Brevibacillus/*classification/genetics/isolation & purification, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Oryza, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
17357Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23904)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23904
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29400Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2580728776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80678Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404432.1StrainInfo: A central database for resolving microbial strain identifiers