Strain identifier

BacDive ID: 11437

Type strain: Yes

Species: Brevibacillus levickii

Strain Designation: R-12315, B-1657

Strain history: CIP <- 2004, NA. Logan, Caledonian Univ., Glasgow, Scotland, UK: strain R-12315

NCBI tax ID(s): 278990 (species)

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General

@ref: 7773

BacDive-ID: 11437

DSM-Number: 18918

keywords: 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic, Gram-variable, motile, rod-shaped

description: Brevibacillus levickii R-12315 is a microaerophile, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 278990
  • Matching level: species

strain history

@refhistory
7773<- CIP <- N. A. Logan; R-12315
120588CIP <- 2004, NA. Logan, Caledonian Univ., Glasgow, Scotland, UK: strain R-12315

doi: 10.13145/bacdive11437.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Brevibacillus
  • species: Brevibacillus levickii
  • full scientific name: Brevibacillus levickii Allan et al. 2005

@ref: 7773

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Brevibacillus

species: Brevibacillus levickii

full scientific name: Brevibacillus levickii Allan et al. 2005

strain designation: R-12315, B-1657

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31358variable3.5 µm0.75 µmrod-shapedyes
120588positiverod-shapedno

colony morphology

  • @ref: 120588

pigmentation

  • @ref: 31358
  • production: no

Culture and growth conditions

culture medium

  • @ref: 7773
  • name: BREVIBACILLUS LEVICKII MEDIUM (DSMZ Medium 1064)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1064
  • composition: Name: BREVIBACILLUS LEVICKII MEDIUM (DSMZ Medium 1064) Composition: Agar 18.0 g/l Yeast extract 2.0 g/l KH2PO4 1.5 g/l (NH4)2SO4 1.25 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.0125 g/l MnSO4 x H2O 0.0025 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7773positivegrowth30-37mesophilic
31358positivegrowth15-55
31358positiveoptimum42.5thermophilic
120588positivegrowth30-37mesophilic
120588nogrowth10psychrophilic
120588nogrowth22psychrophilic
120588nogrowth45thermophilic
120588nogrowth55thermophilic

culture pH

@refabilitytypepH
31358positivegrowth4.5-6.5
31358positiveoptimum5.25
120588positivegrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31358microaerophile
120588obligate aerobe

spore formation

@refspore formation
31358yes
120588yes

halophily

@refsaltgrowthtested relationconcentration
31358NaClpositiveoptimum<2 %
120588NaClpositivegrowth0-2 %
120588NaClnogrowth4 %
120588NaClnogrowth6 %
120588NaClnogrowth8 %
120588NaClnogrowth10 %

observation

  • @ref: 31358
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3135815963ribitol+carbon source
3135816449alanine+carbon source
3135818403L-arabitol+carbon source
3135829016arginine+carbon source
3135835391aspartate+carbon source
3135816947citrate+carbon source
3135828757fructose+carbon source
3135828260galactose+carbon source
3135824175galacturonate+carbon source
313585291gelatin+carbon source
3135824265gluconate+carbon source
3135817234glucose+carbon source
3135829987glutamate+carbon source
3135817754glycerol+carbon source
3135824996lactate+carbon source
3135817716lactose+carbon source
3135825115malate+carbon source
3135817306maltose+carbon source
3135829864mannitol+carbon source
3135837684mannose+carbon source
31358506227N-acetylglucosamine+carbon source
3135826271proline+carbon source
3135817148putrescine+carbon source
3135833942ribose+carbon source
3135830911sorbitol+carbon source
3135830031succinate+carbon source
3135817992sucrose+carbon source
3135827082trehalose+carbon source
3135816296D-tryptophan+carbon source
313584853esculin+hydrolysis
3135817632nitrate+reduction
12058816947citrate-carbon source
1205884853esculin-hydrolysis
120588606565hippurate-hydrolysis
12058817632nitrate-reduction
12058816301nitrite-reduction
12058817632nitrate-respiration

metabolite production

  • @ref: 120588
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12058815688acetoin-
12058817234glucose-

enzymes

@refvalueactivityec
31358catalase+1.11.1.6
31358gelatinase+
120588oxidase+
120588beta-galactosidase-3.2.1.23
120588alcohol dehydrogenase-1.1.1.1
120588gelatinase+/-
120588amylase-
120588DNase-
120588caseinase-3.4.21.50
120588catalase-1.11.1.6
120588tween esterase-
120588gamma-glutamyltransferase-2.3.2.2
120588lecithinase-
120588lipase-
120588lysine decarboxylase-4.1.1.18
120588ornithine decarboxylase-4.1.1.17
120588urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120588-+++-++----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120588+++----------------+++----+----+-+---------++-++++++++++++++++++-+-+-----+-+++-++++---+++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
7773soilMt Melbourne, NW slopeAustralia and Oceania
120588Environment, SoilMount Melbourne, Slope, Victoria landAntarcticaAntarcticaATA

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
77731Risk group (German classification)
1205881Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7773
  • description: Brevibacillus levickii partial 16S rRNA gene, type strain LMG 22481 T
  • accession: AJ715378
  • length: 1489
  • database: ena
  • NCBI tax ID: 278990

GC content

@refGC-content
777348.3-50.3
3135850.3

External links

@ref: 7773

culture collection no.: DSM 18918, CIP 108307, KCTC 13000, LMG 22481, Logan B-1657

straininfo link

  • @ref: 80675
  • straininfo: 131841

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15879231Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica.Allan RN, Lebbe L, Heyrman J, De Vos P, Buchanan CJ, Logan NAInt J Syst Evol Microbiol10.1099/ijs.0.63397-02005Amino Acid Transport Systems, Antarctic Regions, Bacterial Proteins/analysis, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Genes, rRNA, Glutamic Acid/metabolism, Gram-Positive Endospore-Forming Rods/*classification/cytology/*isolation & purification/physiology, Hot Temperature, Hydrogen-Ion Concentration, Ion Transport/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Proteome/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyEnzymology
Phylogeny24105946Brevibacillus fulvus sp. nov., isolated from a compost pile.Hatayama K, Shoun H, Ueda Y, Nakamura AInt J Syst Evol Microbiol10.1099/ijs.0.051052-02013Bacterial Typing Techniques, Base Composition, Brevibacillus/*classification/genetics/isolation & purification, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Refuse Disposal, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7773Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18918)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18918
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31358Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2767128776041
68382Automatically annotated from API zym
80675Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID131841.1StrainInfo: A central database for resolving microbial strain identifiers
120588Curators of the CIPCollection of Institut Pasteur (CIP 108307)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108307