Strain identifier

BacDive ID: 11436

Type strain: Yes

Species: Brevibacillus invocatus

Strain Designation: B2156, C3774, B2156T

Strain history: CIP <- 2001, Dr. N.A. Logan, Caledonian Univ., Glasgow, UK, strain: B2156T

NCBI tax ID(s): 173959 (species)

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General

@ref: 6444

BacDive-ID: 11436

DSM-Number: 16423

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, motile

description: Brevibacillus invocatus B2156 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from antibiotic fermenter.

NCBI tax id

  • NCBI tax id: 173959
  • Matching level: species

strain history

@refhistory
6444<- CIP, Paris, France <- N. A. Logan
396172001, Dr. N.A. Logan, Caledonian Univ., Glasgow, UK, strain: B2156T
67770CIP 106911 <-- N. A. Logan B2156.
120278CIP <- 2001, Dr. N.A. Logan, Caledonian Univ., Glasgow, UK, strain: B2156T

doi: 10.13145/bacdive11436.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Brevibacillus
  • species: Brevibacillus invocatus
  • full scientific name: Brevibacillus invocatus Logan et al. 2002

@ref: 6444

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Brevibacillus

species: Brevibacillus invocatus

full scientific name: Brevibacillus invocatus Logan et al. 2002

strain designation: B2156, C3774, B2156T

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.19
69480100positive
120278nonegativerod-shaped

colony morphology

  • @ref: 120278
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6444CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
39617MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120278CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6444positivegrowth30mesophilic
39617positivegrowth30mesophilic
67770positivegrowth30mesophilic
120278positivegrowth22-45
120278nogrowth10psychrophilic
120278nogrowth55thermophilic

culture pH

  • @ref: 120278
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 120278
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481yes97
69480yes100

halophily

@refsaltgrowthtested relationconcentration
120278NaClpositivegrowth0-2 %
120278NaClnogrowth4 %
120278NaClnogrowth6 %
120278NaClnogrowth8 %
120278NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12027816947citrate-carbon source
1202784853esculin-hydrolysis
120278606565hippurate+hydrolysis
12027817632nitrate-reduction
12027816301nitrite-reduction
12027817632nitrate-respiration

metabolite production

  • @ref: 120278
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12027815688acetoin-
12027817234glucose-

enzymes

@refvalueactivityec
120278oxidase+
120278beta-galactosidase-3.2.1.23
120278alcohol dehydrogenase-1.1.1.1
120278gelatinase-
120278amylase-
120278DNase+
120278caseinase-3.4.21.50
120278catalase+1.11.1.6
120278tween esterase+
120278gamma-glutamyltransferase+2.3.2.2
120278lecithinase-
120278lipase-
120278lysine decarboxylase-4.1.1.18
120278ornithine decarboxylase-4.1.1.17
120278urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120278-+++----+-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120278-------------------+---+---------+---------------------------++-++-------+------------+-+-++------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
6444antibiotic fermenterUnited KingdomGBREurope
67770Antibiotic fermenter
120278Other, Antibiotic fermenter contaminantUnited KingdomGBREuropeEngland1996

isolation source categories

Cat1Cat2Cat3
#Engineered
#Infection#Patient#Antibiotic treatment

taxonmaps

  • @ref: 69479
  • File name: preview.99_1006.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_95;96_577;97_674;98_783;99_1006&stattab=map
  • Last taxonomy: Brevibacillus
  • 16S sequence: AF378232
  • Sequence Identity:
  • Total samples: 646
  • soil counts: 304
  • aquatic counts: 105
  • animal counts: 138
  • plant counts: 99

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64441Risk group (German classification)
1202781Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brevibacillus invocatus strain LMG 18962 16S ribosomal RNA gene, partial sequenceAF3782321488ena173959
20218Brevibacillus invocatus gene for 16S rRNA, partial sequence, strain:NCIMB 13772TAB1127181488ena173959

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacillus invocatus strain JCM 12215173959.4wgspatric173959
66792Brevibacillus invocatus JCM 122152851194356draftimg173959
67770Brevibacillus invocatus JCM 12215GCA_003710915contigncbi173959

GC content

  • @ref: 67770
  • GC-content: 49.8
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes97no
motileyes86.222no
gram-positiveyes82.643no
anaerobicno98.191no
aerobicyes88.827no
halophileno94.165no
spore-formingyes96.316no
thermophileno90.379yes
glucose-utilyes89.959no
flagellatedyes50.658no
glucose-fermentno95.619no

External links

@ref: 6444

culture collection no.: DSM 16423, CIP 106911, LMG 18962, NCIMB 13772, JCM 12215

straininfo link

  • @ref: 80674
  • straininfo: 13160

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12054263Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov..Logan NA, Forsyth G, Lebbe L, Goris J, Heyndrickx M, Balcaen A, Verhelst A, Falsen E, Ljungh A, Hansson HB, De Vos PInt J Syst Evol Microbiol10.1099/00207713-52-3-9532002Bacillus/*classification/genetics/metabolism, Bacterial Infections/microbiology, Bacterial Typing Techniques, DNA, Ribosomal/analysis, Dairy Products/microbiology, Gram-Negative Aerobic Bacteria/*classification/genetics/metabolism, Humans, Industrial Microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Restriction Mapping, Sequence Analysis, DNA, Spores, Bacterial, Water MicrobiologyMetabolism
Phylogeny19406823Brevibacillus panacihumi sp. nov., a beta-glucosidase-producing bacterium.Kim MK, Sathiyaraj S, Pulla RK, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.001248-02009Bacterial Typing Techniques, DNA, Bacterial/analysis/isolation & purification, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genotype, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification/physiology, Korea, Molecular Sequence Data, *Panax, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, beta-Glucosidase/*biosynthesisGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6444Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16423)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16423
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39617Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19094
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80674Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13160.1StrainInfo: A central database for resolving microbial strain identifiers
120278Curators of the CIPCollection of Institut Pasteur (CIP 106911)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106911