Strain identifier

BacDive ID: 11421

Type strain: Yes

Species: Brevibacillus borstelensis

Strain Designation: B4, NRS-818

Strain history: CIP <- 1995, O. Shida, Higeta Shoyu Lab., Chiba, Japan: strain NRS-818 <- NRRL <- J.R. Porter

NCBI tax ID(s): 45462 (species)

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General

@ref: 2604

BacDive-ID: 11421

DSM-Number: 6347

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile

description: Brevibacillus borstelensis B4 is an aerobe, spore-forming, Gram-positive bacterium of the family Paenibacillaceae.

NCBI tax id

  • NCBI tax id: 45462
  • Matching level: species

strain history

@refhistory
2604<- NRRL; NRS 818 <- J.R. Porter, Univ. of Iowa, 1940, (Bacillus borstelensis) B4, thermophilic strain <- G. Bredemann
67770O. Shida <-- NRRL NRS-818 <-- N. R. Smith <-- J. R. Porter B4.
116899CIP <- 1995, O. Shida, Higeta Shoyu Lab., Chiba, Japan: strain NRS-818 <- NRRL <- J.R. Porter

doi: 10.13145/bacdive11421.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Brevibacillus
  • species: Brevibacillus borstelensis
  • full scientific name: Brevibacillus borstelensis (Shida et al. 1995 ex Porter 1940) Shida et al. 1996

  • synonyms

    • @ref: 20215
    • synonym: Bacillus borstelensis

@ref: 2604

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Brevibacillus

species: Brevibacillus borstelensis

full scientific name: Brevibacillus borstelensis (Shida et al. 1995) Shida et al. 1996

strain designation: B4, NRS-818

type strain: yes

Morphology

cell morphology

  • @ref: 116899
  • gram stain: positive
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2604NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39035MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116899CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
2604positivegrowth30
39035positivegrowth30
59095positivegrowth37
67770positivegrowth30
116899positivegrowth30-45
116899nogrowth10
116899nogrowth55

culture pH

  • @ref: 116899
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59095aerobe
116899obligate aerobe

spore formation

@refspore formationconfidence
116899yes
69481yes95

halophily

@refsaltgrowthtested relationconcentration
116899NaClpositivegrowth0 %
116899NaClnogrowth2 %
116899NaClnogrowth4 %
116899NaClnogrowth6 %
116899NaClnogrowth8 %
116899NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose-fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
116899nitrate+reduction17632
116899nitrite-reduction16301
116899nitrate-respiration17632

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11689935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11689915688acetoin-
11689917234glucose-

enzymes

@refvalueactivityec
68368cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116899oxidase-
116899alcohol dehydrogenase-1.1.1.1
116899gelatinase+
116899amylase-
116899DNase+
116899caseinase+3.4.21.50
116899catalase+1.11.1.6
116899tween esterase-
116899lecithinase-
116899lysine decarboxylase-4.1.1.18
116899ornithine decarboxylase-4.1.1.17
116899urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    59095C14:0 ISO11.813.618
    59095C15:0 ANTEISO28.714.711
    59095C15:0 ISO39.414.621
    59095C16:0 iso6.815.626
    59095C16:1 ω11c2.315.757
    59095C16:1 ω7c alcohol6.415.386
    59095C17:1 ISO I/C16:0 DMA116.481
    59095C17:1 ω10c ISO3.616.387
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
59095---------+----------+

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
59095--------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116899-+-----------------+-----------------------------+++----+---+------------------++-+-----+-+++++----

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
39035BorstelGermanyDEUEurope
59095BorstelGermanyDEUEuropeSoil
67770BorstelGermanyDEUEuropeSoil
116899BorstelGermanyDEUEuropeEnvironment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3923.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_95;96_577;97_2409;98_2979;99_3923&stattab=map
  • Last taxonomy: Brevibacillus borstelensis subclade
  • 16S sequence: AB680943
  • Sequence Identity:
  • Total samples: 5049
  • soil counts: 2107
  • aquatic counts: 611
  • animal counts: 965
  • plant counts: 1366

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
26041Risk group (German classification)
1168991Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brevibacillus borstelensis clone EE-4_F 16S ribosomal RNA gene and 16S-23S internal transcribed spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequenceAF478092617nuccore45462
20218Brevibacillus borstelensis clone Bborst-1 16S ribosomal RNA gene and 16S-23S internal transcribed spacer, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequenceAF478093678nuccore45462
20218Brevibacillus borstelensis gene for 16S rRNA, partial sequence, strain:DSM 6347TAB1127211486nuccore45462
20218Brevibacillus borstelensis gene for 16S rRNA, partial sequence, strain: NBRC 15714AB6809431461nuccore45462
20218Brevibacillus borstelensis DNA for 16S ribosomal RNAD784561419nuccore45462

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacillus borstelensis strain NBRC 1571445462.11wgspatric45462
67770Brevibacillus borstelensis NBRC 15714GCA_006540165contigncbi45462
67770Brevibacillus borstelensis NRRL NRS 818GCA_003710865contigncbi45462

GC content

@refGC-contentmethod
260452.4
6777051.3high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes95no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes54.716no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no94.549no
69480spore-formingspore-formingAbility to form endo- or exosporesyes86.733no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes71.076yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno85.418no
69480flagellatedmotile2+Ability to perform flagellated movementyes82.407no

External links

@ref: 2604

culture collection no.: DSM 6347, ATCC 51668, CIP 104545, IFO 15714, JCM 9022, NBRC 15714, CCUG 51058, NRRL NRS-818, LMG 16009, NRS 818, CGMCC 1.3102

straininfo link

@refstraininfo
806548287
80655312202

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity33772669Groundwater hydro-geochemistry, quality, microbiology and human health risk assessment in semi-arid area of Rajasthan, India: a chemometric approach.Khan N, Malik A, Nehra KEnviron Monit Assess10.1007/s10661-021-08979-22021Bacillus, Brevibacillus, Child, Environmental Monitoring, Female, *Groundwater, Humans, India, Male, RNA, Ribosomal, 16S, Risk Assessment, *Water Pollutants, Chemical/analysis, Water QualityPhylogeny
Phylogeny34762578Brevibacillus composti sp. nov., isolated from hyperthermophilic compost.Tang R, Zhang Q, Ye Y, Yang S, Fu T, Liu GH, Zhou SGInt J Syst Evol Microbiol10.1099/ijsem.0.0050982021Bacterial Typing Techniques, Base Composition, *Brevibacillus/classification/isolation & purification, China, *Composting, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2604Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6347)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6347
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39035Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16465
59095Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51058)https://www.ccug.se/strain?id=51058
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80654Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8287.1StrainInfo: A central database for resolving microbial strain identifiers
80655Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID312202.1StrainInfo: A central database for resolving microbial strain identifiers
116899Curators of the CIPCollection of Institut Pasteur (CIP 104545)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104545