Strain identifier

BacDive ID: 11402

Type strain: Yes

Species: Brevibacillus brevis

Strain Designation: 27B, 604

Strain history: CIP <- 1952, N.R. Smith, New Jersey Agri.: strain 604 <- J.R. Porter <- NCTC <- W.W. Ford: strain 27B

NCBI tax ID(s): 1393 (species)

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General

@ref: 2031

BacDive-ID: 11402

DSM-Number: 30

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, motile, human pathogen

description: Brevibacillus brevis 27B is an aerobe, spore-forming, mesophilic human pathogen of the family Paenibacillaceae.

NCBI tax id

  • NCBI tax id: 1393
  • Matching level: species

strain history

@refhistory
2031<- ATCC <- N.R. Smith, 604 <- J.R. Porter <- NCTC <- W.W. Ford, 27B
67770DSM 30 <-- ATCC 8246 <-- N. R. Smith 604 <-- J. R. Porter <-- NCTC 2611 <-- W. W. Ford 27B.
117035CIP <- 1952, N.R. Smith, New Jersey Agri.: strain 604 <- J.R. Porter <- NCTC <- W.W. Ford: strain 27B

doi: 10.13145/bacdive11402.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Brevibacillus
  • species: Brevibacillus brevis
  • full scientific name: Brevibacillus brevis (Migula 1900) Shida et al. 1996

  • synonyms

    • @ref: 20215
    • synonym: Bacillus brevis

@ref: 2031

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Brevibacillus

species: Brevibacillus brevis

full scientific name: Brevibacillus brevis (Migula 1900) Shida et al. 1996

strain designation: 27B, 604

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes97.085
69480100positive
117035yespositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37350MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
2031NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
117035CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
2031positivegrowth30mesophilic
37350positivegrowth30mesophilic
44983positivegrowth37mesophilic
67770positivegrowth30mesophilic
117035positivegrowth22-37
117035nogrowth10psychrophilic
117035nogrowth45thermophilic
117035nogrowth55thermophilic

culture pH

  • @ref: 117035
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44983aerobe
117035obligate aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100
117035yes

halophily

@refsaltgrowthtested relationconcentration
117035NaClpositivegrowth0 %
117035NaClnogrowth2 %
117035NaClnogrowth4 %
117035NaClnogrowth6 %
117035NaClnogrowth8 %
117035NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
117035citrate-carbon source16947
117035nitrate+reduction17632
117035nitrite-reduction16301
117035nitrate-respiration17632
68379nitrate+reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin+hydrolysis5291
68379D-ribose+fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose+fermentation17306
68379lactose-fermentation17716
68379sucrose+fermentation17992
68379glycogen+fermentation28087

metabolite production

  • @ref: 117035
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 117035
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68379catalase+1.11.1.6
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
117035oxidase+
117035beta-galactosidase+3.2.1.23
117035alcohol dehydrogenase-1.1.1.1
117035gelatinase-
117035amylase-
117035DNase+
117035caseinase-3.4.21.50
117035catalase-1.11.1.6
117035tween esterase+
117035gamma-glutamyltransferase+2.3.2.2
117035lecithinase+
117035lysine decarboxylase-4.1.1.18
117035ornithine decarboxylase-4.1.1.17
117035urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
44983++++---++-+-+--+-+++

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117035-+++----+-+---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117035+/-----+/------+/------+/--------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117035++++---+-++--+-++++---+--+-----+-+-+--------------+--+-----+++----------------------+---+++++++----

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
2031yes, in single cases1Risk group (German classification)
1170351Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brevibacillus brevis clone JG-1F 16S ribosomal RNA gene, and 16S-23S internal transcribed spacer, partial sequenceAF478094319ena1393
20218B.brevis 16S rRNAM101111557ena1393
20218Brevibacillus brevis gene for 16S rRNA, partial sequenceAB1015931486ena1393
20218Brevibacillus brevis gene for 16S rRNA, partial sequenceAB006941263ena1393
20218Brevibacillus brevis DNA for 16S ribosomal RNAD784571419ena1393
20218Brevibacillus brevis gene for 16S rRNA, partial sequenceAB2717561461ena1393
20218B.brevis 16S ribosomal RNAX606121417ena1393
20218Bacillus subtilis strain BNRS604 16S ribosomal RNA gene, partial sequenceHM133978230ena1423

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacillus brevis NCTC2611GCA_900637055completencbi1393
66792Brevibacillus brevis strain NBRC 153041393.23wgspatric1393
66792Brevibacillus brevis strain NCTC26111393.21completepatric1393
66792Brevibacillus brevis NCTC26112843783720completeimg1393
67770Brevibacillus brevis NBRC 15304GCA_006539845contigncbi1393
67770Brevibacillus brevis NRRL NRS-604GCA_003012835contigncbi1393
67770Brevibacillus brevis DSM 30GCA_003385915scaffoldncbi1393

GC content

@refGC-contentmethod
203147.4Buoyant density centrifugation (BD)
203147.3thermal denaturation, midpoint method (Tm)
6777048.7Buoyant density centrifugation (BD)
6777047high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedyes69.488no
gram-positiveyes71.868no
anaerobicno98.396yes
aerobicyes85.826no
halophileno91.115no
spore-formingyes95.82no
thermophileno98.629yes
glucose-utilyes88.344no
motileyes89.249no
glucose-fermentno93.917no

External links

@ref: 2031

culture collection no.: DSM 30, ATCC 8246, CCM 2050, JCM 2503, LMG 16703, NCIB 9372, NCTC 2611, CCUG 7413, NRS 604, BCRC 14682, CECT 5, CGMCC 1.3098, CIP 52.86, IFO 15304, KCTC 3743, LMG 7123, NBRC 15304, NCIMB 9372, NRRL B-14602, NRRL NRS-604, NRRL NRS-819, VKM B-503, NCDO 1770

straininfo link

@refstraininfo
806328291
80633312199

literature

  • topic: Phylogeny
  • Pubmed-ID: 26585900
  • title: Brevibacillus gelatini sp. nov., isolated from a hot spring.
  • authors: Inan K, Ozer A, Ibrahim Guler H, Osman Belduz A, Canakci S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000780
  • year: 2015

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2031Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30)https://www.dsmz.de/collection/catalogue/details/culture/DSM-30
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37350Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13500
44983Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7413)https://www.ccug.se/strain?id=7413
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80632Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8291.1StrainInfo: A central database for resolving microbial strain identifiers
80633Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID312199.1StrainInfo: A central database for resolving microbial strain identifiers
117035Curators of the CIPCollection of Institut Pasteur (CIP 52.86)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.86