Strain identifier

BacDive ID: 11401

Type strain: Yes

Species: Aneurinibacillus terranovensis

Strain Designation: R-12870, B-1599

Strain history: CIP <- 2004, NA. Logan, Caledonian Univ., Glasgow, Scotland, UK: strain R-12870

NCBI tax ID(s): 1121002 (strain), 278991 (species)

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General

@ref: 7774

BacDive-ID: 11401

DSM-Number: 18919

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, thermophilic, Gram-variable, motile, rod-shaped

description: Aneurinibacillus terranovensis R-12870 is a microaerophile, spore-forming, thermophilic bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
278991species
1121002strain

strain history

@refhistory
7774<- CIP <- N. A. Logan; R-12870
123467CIP <- 2004, NA. Logan, Caledonian Univ., Glasgow, Scotland, UK: strain R-12870

doi: 10.13145/bacdive11401.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Aneurinibacillus
  • species: Aneurinibacillus terranovensis
  • full scientific name: Aneurinibacillus terranovensis Allan et al. 2005

@ref: 7774

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Aneurinibacillus

species: Aneurinibacillus terranovensis

full scientific name: Aneurinibacillus terranovensis Allan et al. 2005

strain designation: R-12870, B-1599

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31358variable5 µm0.9 µmrod-shapedyes
69480yes97.322
69480positive99.999
123467positiverod-shapedyes

colony morphology

  • @ref: 123467

pigmentation

  • @ref: 31358
  • production: no

Culture and growth conditions

culture medium

  • @ref: 7774
  • name: BREVIBACILLUS LEVICKII MEDIUM (DSMZ Medium 1064)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1064
  • composition: Name: BREVIBACILLUS LEVICKII MEDIUM (DSMZ Medium 1064) Composition: Agar 18.0 g/l Yeast extract 2.0 g/l KH2PO4 1.5 g/l (NH4)2SO4 1.25 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.0125 g/l MnSO4 x H2O 0.0025 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7774positivegrowth40thermophilic
31358positivegrowth20-55
31358positiveoptimum41thermophilic
123467positivegrowth30-45
123467nogrowth10psychrophilic
123467nogrowth22psychrophilic
123467nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
31358positivegrowth3.5-6.0acidophile
31358positiveoptimum5.25
123467nogrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 31358
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
31358yes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
31358NaClpositiveoptimum<2 %
123467NaClpositivegrowth0 %
123467NaClnogrowth2 %
123467NaClnogrowth4 %
123467NaClnogrowth6 %
123467NaClnogrowth8 %
123467NaClnogrowth10 %

observation

  • @ref: 31358
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3135815963ribitol+carbon source
3135816449alanine+carbon source
3135818403L-arabitol+carbon source
3135829016arginine+carbon source
3135835391aspartate+carbon source
3135816947citrate+carbon source
3135828757fructose+carbon source
3135828260galactose+carbon source
3135824175galacturonate+carbon source
313585291gelatin+carbon source
3135824265gluconate+carbon source
313585417glucosamine+carbon source
3135817234glucose+carbon source
3135829987glutamate+carbon source
3135817754glycerol+carbon source
3135824996lactate+carbon source
3135817716lactose+carbon source
3135825115malate+carbon source
3135829864mannitol+carbon source
3135837684mannose+carbon source
3135817268myo-inositol+carbon source
31358506227N-acetylglucosamine+carbon source
3135826271proline+carbon source
3135817148putrescine+carbon source
3135833942ribose+carbon source
3135830911sorbitol+carbon source
3135830031succinate+carbon source
3135817992sucrose+carbon source
3135827082trehalose+carbon source
313584853esculin+hydrolysis
3135817632nitrate+reduction
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12346716947citrate-carbon source
1234674853esculin+hydrolysis
123467606565hippurate-hydrolysis
12346717632nitrate-reduction
12346716301nitrite-reduction
12346717632nitrate+respiration

metabolite production

  • @ref: 123467
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12346715688acetoin-
12346717234glucose-

enzymes

@refvalueactivityec
31358catalase+1.11.1.6
31358gelatinase+
123467oxidase-
123467beta-galactosidase-3.2.1.23
123467alcohol dehydrogenase+1.1.1.1
123467gelatinase-
123467amylase-
123467DNase-
123467caseinase-3.4.21.50
123467catalase+1.11.1.6
123467tween esterase-
123467gamma-glutamyltransferase-2.3.2.2
123467lecithinase-
123467lipase-
123467lysine decarboxylase-4.1.1.18
123467ornithine decarboxylase-4.1.1.17
123467urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123467--++------++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123467----------+/--------------+/------------------------+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
7774soilMt. Melbourne, NW slopeAustralia and Oceania
123467Environment, SoilMount Melbourne, Victoria landAntarcticaAntarcticaATA

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
77741Risk group (German classification)
1234671Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7774
  • description: Aneurinibacillus terranovensis partial 16S rRNA gene, type strain LMG 22483 T
  • accession: AJ715385
  • length: 1415
  • database: ena
  • NCBI tax ID: 278991

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aneurinibacillus terranovensis DSM 18919GCA_000430625scaffoldncbi1121002
66792Aneurinibacillus terranovensis DSM 189191121002.4wgspatric1121002
66792Aneurinibacillus terranovensis DSM 189192524614808draftimg1121002

GC content

@refGC-content
777443.2-44.6
3135843.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes90.516yes
flagellatedyes58.331no
gram-positiveno61.836yes
anaerobicno94.278yes
aerobicyes57.24no
halophileno96.983no
spore-formingyes96.847no
thermophileyes79.693no
glucose-utilyes88.642yes
glucose-fermentno86.907no

External links

@ref: 7774

culture collection no.: DSM 18919, CIP 108308, KCTC 13001, LMG 22483, Logan B-1599

straininfo link

  • @ref: 80631
  • straininfo: 131843

literature

  • topic: Phylogeny
  • Pubmed-ID: 15879231
  • title: Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica.
  • authors: Allan RN, Lebbe L, Heyrman J, De Vos P, Buchanan CJ, Logan NA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63397-0
  • year: 2005
  • mesh: Amino Acid Transport Systems, Antarctic Regions, Bacterial Proteins/analysis, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Genes, rRNA, Glutamic Acid/metabolism, Gram-Positive Endospore-Forming Rods/*classification/cytology/*isolation & purification/physiology, Hot Temperature, Hydrogen-Ion Concentration, Ion Transport/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Proteome/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7774Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18919)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18919
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31358Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2767128776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80631Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID131843.1StrainInfo: A central database for resolving microbial strain identifiers
123467Curators of the CIPCollection of Institut Pasteur (CIP 108308)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108308