Strain identifier

BacDive ID: 11263

Type strain: Yes

Species: Marinactinospora thermotolerans

Strain history: DSM 45154 <-- S.-K. Tang and W.-J. Li SCSIO 00652 <-- X.-P. Tian et al..

NCBI tax ID(s): 1122192 (strain), 531310 (species)

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General

@ref: 15448

BacDive-ID: 11263

DSM-Number: 45154

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile

description: Marinactinospora thermotolerans DSM 45154 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from deep-sea sediment at about 3865 m depth.

NCBI tax id

NCBI tax idMatching level
1122192strain
531310species

strain history

@refhistory
15448<- S.-K. Tang and W.-J. Li, YIM; SCSIO 00652 <- X.-P. Tian et al.
67770DSM 45154 <-- S.-K. Tang and W.-J. Li SCSIO 00652 <-- X.-P. Tian et al..

doi: 10.13145/bacdive11263.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Nocardiopsidaceae
  • genus: Marinactinospora
  • species: Marinactinospora thermotolerans
  • full scientific name: Marinactinospora thermotolerans Tian et al. 2009

@ref: 15448

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiopsaceae

genus: Marinactinospora

species: Marinactinospora thermotolerans

full scientific name: Marinactinospora thermotolerans Tian et al. 2009

type strain: yes

Morphology

cell morphology

  • @ref: 29062
  • gram stain: positive
  • motility: yes

pigmentation

  • @ref: 29062
  • production: no

multimedia

@refmultimedia contentcaptionintellectual property rights
15448https://www.dsmz.de/microorganisms/photos/DSM_45154.jpgMedium 535 28°C© Leibniz-Institut DSMZ
66793EM_DSM_45154_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45154_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45154_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45154_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_45154_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15448GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
15448GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
15448positivegrowth28
29062positivegrowth10-55
29062positiveoptimum28
67770positivegrowth28

culture pH

@refabilitytypepHPH range
29062positivegrowth06-09alkaliphile
29062positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 29062
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
29062yes
69481yes100

halophily

@refsaltgrowthtested relationconcentration
29062NaClpositivegrowth0-5 %
29062NaClpositiveoptimum0.5 %

observation

@refobservation
29062aggregates in chains
67770quinones: MK-10(H8), MK-11(H8), MK-11(H10)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2906217057cellobiose+carbon source
2906228757fructose+carbon source
2906228260galactose+carbon source
2906217234glucose+carbon source
2906217306maltose+carbon source
2906229864mannitol+carbon source
2906237684mannose+carbon source
2906226546rhamnose+carbon source
2906233942ribose+carbon source
2906230911sorbitol+carbon source
2906217992sucrose+carbon source
2906253424tween 20+carbon source
2906253423tween 40+carbon source
2906253425tween 60+carbon source
2906253426tween 80+carbon source
2906216199urea+carbon source
2906218222xylose+carbon source
2906217632nitrate+reduction

enzymes

@refvalueactivityec
29062catalase+1.11.1.6
29062cytochrome oxidase+1.9.3.1
29062urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
15448deep-sea sediment at about 3865 m depthnorthern South China Sea (17° 58.742' N 116° 00.228' E)ChinaCHNAsia17.9792116.004
67770Deep-sea sediment (black soft mud at a depth of 3,865 m)ChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_7117.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_152;96_3336;97_4093;98_5235;99_7117&stattab=map
  • Last taxonomy: Marinactinospora
  • 16S sequence: EU698029
  • Sequence Identity:
  • Total samples: 361
  • soil counts: 219
  • aquatic counts: 18
  • animal counts: 52
  • plant counts: 72

Safety information

risk assessment

  • @ref: 15448
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15448
  • description: Marinactinospora thermotolerans strain SCSIO 00652 16S ribosomal RNA gene, partial sequence
  • accession: EU698029
  • length: 1443
  • database: nuccore
  • NCBI tax ID: 531310

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinactinospora thermotolerans DSM 451541122192.6wgspatric1122192
66792Marinactinospora thermotolerans DSM 451542524614583draftimg1122192
67770Marinactinospora thermotolerans DSM 45154GCA_900167435scaffoldncbi1122192

GC content

@refGC-contentmethod
1544872high performance liquid chromatography (HPLC)
2906272

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes84.174yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.931yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes80.766no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes89.6yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno92.884no
69480flagellatedmotile2+Ability to perform flagellated movementno88no

External links

@ref: 15448

culture collection no.: DSM 45154, CCTCC AA 208041, JCM 16903, SCSIO 00652, NBRC 110046

straininfo link

  • @ref: 80496
  • straininfo: 405821

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19406773Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea.Tian XP, Tang SK, Dong JD, Zhang YQ, Xu LH, Zhang S, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.005231-02009Actinomycetales/chemistry/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Geologic Sediments/*microbiology, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Species Specificity, Vitamin K 2/analysisGenetics
Phylogeny21216917Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment.Chang X, Liu W, Zhang XHInt J Syst Evol Microbiol10.1099/ijs.0.027383-02011Actinobacteria/*classification/genetics/*isolation & purification/metabolism, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, Seawater/*microbiology, Sodium Chloride/metabolismMetabolism
Enzymology21977916Antimalarial beta-carboline and indolactam alkaloids from Marinactinospora thermotolerans, a deep sea isolate.Huang H, Yao Y, He Z, Yang T, Ma J, Tian X, Li Y, Huang C, Chen X, Li W, Zhang S, Zhang C, Ju JJ Nat Prod10.1021/np200399t2011Actinomycetales/*chemistry, Alkaloids/chemistry/*isolation & purification/pharmacology, Antimalarials/chemistry/*isolation & purification/pharmacology, Antineoplastic Agents/chemistry/*isolation & purification/pharmacology, Carbolines/chemistry/*isolation & purification/pharmacology, Crystallography, X-Ray, Drug Screening Assays, Antitumor, Humans, Marine Biology, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Plasmodium falciparum/drug effectsPhylogeny
Genetics22064543Discovery and engineered overproduction of antimicrobial nucleoside antibiotic A201A from the deep-sea marine actinomycete Marinactinospora thermotolerans SCSIO 00652.Zhu Q, Li J, Ma J, Luo M, Wang B, Huang H, Tian X, Li W, Zhang S, Zhang C, Ju JAntimicrob Agents Chemother10.1128/AAC.05278-112011Actinomycetales/enzymology/*genetics, Amino Acid Sequence, Aminoglycosides/biosynthesis, Anti-Bacterial Agents/biosynthesis, Aquatic Organisms, Bacterial Proteins/genetics/metabolism, China, Chromatography, High Pressure Liquid, Computational Biology, Fermentation, *Gene Expression Regulation, Bacterial, Genome-Wide Association Study, Genomic Library, Industrial Microbiology, Metabolic Engineering, Molecular Sequence Data, Multigene Family, Mutation, Oceans and Seas, Oxidoreductases/*genetics/metabolism, Transcription, GeneticBiotechnology
Metabolism22362652Characterization of a single gene cluster responsible for methylpendolmycin and pendolmycin biosynthesis in the deep sea bacterium Marinactinospora thermotolerans.Ma J, Zuo D, Song Y, Wang B, Huang H, Yao Y, Li W, Zhang S, Zhang C, Ju JChembiochem10.1002/cbic.2011007002012Actinomycetales/chemistry/*metabolism, Alkaloids/*biosynthesis/chemistry, Biocatalysis, Computational Biology, Cytochrome P-450 Enzyme System/*genetics/metabolism, Dimethylallyltranstransferase/*genetics/*metabolism, Genetic Engineering, Indoles/chemistry, Lactams/chemistry, Molecular Conformation, Multigene Family/genetics, Peptide Synthases/*genetics/metabolism, Stereoisomerism
Enzymology23215246Marthiapeptide A, an anti-infective and cytotoxic polythiazole cyclopeptide from a 60 L scale fermentation of the deep sea-derived Marinactinospora thermotolerans SCSIO 00652.Zhou X, Huang H, Chen Y, Tan J, Song Y, Zou J, Tian X, Hua Y, Ju JJ Nat Prod10.1021/np300554f2012Actinomycetales/*chemistry, Anti-Bacterial Agents/chemistry/*isolation & purification/*pharmacology, China, Chromatography, High Pressure Liquid, Crystallography, X-Ray, Drug Screening Assays, Antitumor, Female, Gram-Positive Bacteria/drug effects, Hep G2 Cells, Humans, Microbial Sensitivity Tests, Molecular Structure, Oceans and Seas, Peptides, Cyclic/chemistry/*isolation & purification/*pharmacology, Thiazoles/chemistry/*isolation & purification/*pharmacologyPhylogeny
Phylogeny25903844Marinactinospora endophytica sp. nov., isolated from a medicinal plant.Liu MJ, Khieu TN, Gao R, Hozzein WN, Wang HF, Yang W, Nimaichand S, Xiong Z, Duan YQ, Li WJAntonie Van Leeuwenhoek10.1007/s10482-015-0451-12015Actinobacteria/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Stems/microbiology, Plants, Medicinal/*microbiology, RNA, Ribosomal, 16S/genetics, Salsola/microbiology, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureGenetics
Enzymology26754423Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol.Deng D, Zhang Y, Sun A, Liang J, Hu YAppl Biochem Biotechnol10.1007/s12010-016-1980-42016Actinobacteria/*enzymology/genetics, Aquatic Organisms/*enzymology/genetics, Cloning, Molecular, Escherichia coli/genetics, Genome, Bacterial, Lipase/biosynthesis/chemistry/*genetics, Phenylethyl Alcohol/*chemistry, StereoisomerismGenetics
Phylogeny28576760Mathermycin, a Lantibiotic from the Marine Actinomycete Marinactinospora thermotolerans SCSIO 00652.Chen E, Chen Q, Chen S, Xu B, Ju J, Wang HAppl Environ Microbiol10.1128/AEM.00926-172017Actinomycetales/classification/genetics/*isolation & purification/*metabolism, Amino Acid Sequence, Bacillus subtilis/drug effects, Bacterial Proteins/genetics/metabolism, Bacteriocins/*biosynthesis/pharmacology, Biosynthetic Pathways, Peptide Mapping, Seawater/*microbiologyMetabolism
Phylogeny30298376Marinactinospora rubrisoli sp. nov., isolated from red soil.Huang B, Cheng K, Zhang L, Huang Y, Liu CArch Microbiol10.1007/s00203-018-1583-22018Actinomycetales/chemistry/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/chemistry, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/analysis, Soil, *Soil MicrobiologyEnzymology
Enzymology30702740Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs.Zhu Q, Song Y, Huang H, Li Q, Ju JOrg Biomol Chem10.1039/c8ob02852d2019Actinobacteria/*enzymology, Aminoglycosides/*biosynthesis/chemistry, Biocatalysis, Computational Biology, Molecular Structure, Mutation, Oxo-Acid-Lyases/genetics/*metabolismMetabolism
Phylogeny31310190Marinitenerispora sediminis gen. nov., sp. nov., a member of the family Nocardiopsaceae isolated from marine sediment.Ng ZY, Fang BZ, Li WJ, Tan GYAInt J Syst Evol Microbiol10.1099/ijsem.0.0035872019Actinomycetales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Genes, Bacterial, Geologic Sediments/*microbiology, Islands, Malaysia, Multilocus Sequence Typing, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15448Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45154)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45154
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29062Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2549228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80496Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405821.1StrainInfo: A central database for resolving microbial strain identifiers