Strain identifier
BacDive ID: 11251
Type strain: 
Species: Streptomonospora amylolytica
Strain history: <- S.-K. Tang, YIM; YIM 91353 <- M. Cai
NCBI tax ID(s): 505254 (species)
version 8.1 (current version)
General
@ref: 15458
BacDive-ID: 11251
DSM-Number: 45171
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Streptomonospora amylolytica DSM 45171 is an aerobe, spore-forming, mesophilic bacterium that was isolated from saline soil from a salt lake.
NCBI tax id
- NCBI tax id: 505254
- Matching level: species
strain history
- @ref: 15458
- history: <- S.-K. Tang, YIM; YIM 91353 <- M. Cai
doi: 10.13145/bacdive11251.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Nocardiopsidaceae
- genus: Streptomonospora
- species: Streptomonospora amylolytica
- full scientific name: Streptomonospora amylolytica Cai et al. 2009
@ref: 15458
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardiopsaceae
genus: Streptomonospora
species: Streptomonospora amylolytica
full scientific name: Streptomonospora amylolytica Cai et al. 2009
type strain: yes
Morphology
cell morphology
- @ref: 29162
- gram stain: positive
- cell length: 0.5 µm
- cell width: 0.35 µm
- motility: no
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 20173 | Ochre brown (8001) | 10-14 days | ISP 2 |
| 20173 | Beige (1001) | 10-14 days | ISP 3 |
| 20173 | Ochre brown (8001) | 10-14 days | ISP 4 |
| 20173 | Beige (1001) | 10-14 days | ISP 5 |
| 20173 | Ivory (1014) | 10-14 days | ISP 6 |
| 20173 | Beige (1001) | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name |
|---|---|---|
| 20173 | no | ISP 2 |
| 20173 | no | ISP 3 |
| 20173 | no | ISP 4 |
| 20173 | no | ISP 5 |
| 20173 | no | ISP 6 |
| 20173 | no | ISP 7 |
pigmentation
- @ref: 29162
- production: no
multimedia
- @ref: 15458
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45171.jpg
- caption: Medium 1065 37°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 15458 | M1-NOCARDIOPSIS ARABIA MEDIUM (DSMZ Medium 1065) | yes | https://mediadive.dsmz.de/medium/1065 | Name: M1-NOCARDIOPSIS ARABIA MEDIUM (DSMZ Medium 1065) Composition: NaCl 20.0 g/l Agar 18.0 g/l Starch 10.0 g/l Yeast extract 4.0 g/l Peptone 2.0 g/l Sea water |
| 15458 | STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1240.pdf | |
| 20173 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 20173 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 20173 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 20173 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 20173 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 20173 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 15458 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
| 15458 | GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) | yes | https://mediadive.dsmz.de/medium/1159 | Name: GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) Composition: NaCl 100.0 g/l Agar 15.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 15458 | positive | growth | 37 | mesophilic |
| 20173 | positive | optimum | 37 | mesophilic |
| 29162 | positive | growth | 20-45 | |
| 29162 | positive | optimum | 37 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29162 | positive | growth | 5.0-9.0 | alkaliphile |
| 29162 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 29162
- oxygen tolerance: aerobe
spore formation
- @ref: 29162
- spore formation: yes
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 29162 | NaCl | positive | growth | 05-20 % |
| 29162 | NaCl | positive | optimum | 10 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 20173 | 17234 | glucose | + | |
| 20173 | 22599 | arabinose | + | |
| 20173 | 17992 | sucrose | +/- | |
| 20173 | 18222 | xylose | - | |
| 20173 | 17268 | myo-inositol | + | |
| 20173 | 29864 | mannitol | + | |
| 20173 | 28757 | fructose | + | |
| 20173 | 26546 | rhamnose | - | |
| 20173 | 16634 | raffinose | +/- | |
| 20173 | 62968 | cellulose | +/- | |
| 29162 | 22599 | arabinose | + | carbon source |
| 29162 | 29016 | arginine | + | carbon source |
| 29162 | 17057 | cellobiose | + | carbon source |
| 29162 | 17234 | glucose | + | carbon source |
| 29162 | 18240 | 4-hydroxy-L-proline | + | carbon source |
| 29162 | 26271 | proline | + | carbon source |
| 29162 | 26546 | rhamnose | + | carbon source |
| 29162 | 26986 | threonine | + | carbon source |
| 29162 | 16296 | D-tryptophan | + | carbon source |
| 29162 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 29162
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29162 | catalase | + | 1.11.1.6 |
| 29162 | urease | + | 3.5.1.5 |
| 68382 | alkaline phosphatase | - | 3.1.3.1 |
| 68382 | esterase (C 4) | - | |
| 68382 | esterase lipase (C 8) | - | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | - | 3.4.11.1 |
| 68382 | valine arylamidase | - | |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | - | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | - | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | - | 3.2.1.20 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20173 | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 15458
- sample type: saline soil from a salt lake
- geographic location: Xinjiang Province
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Saline |
Safety information
risk assessment
- @ref: 15458
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15458
- description: Streptomonospora amylolytica strain YIM 91353 16S ribosomal RNA gene, partial sequence
- accession: EU442551
- length: 1532
- database: ena
- NCBI tax ID: 505254
GC content
| @ref | GC-content | method |
|---|---|---|
| 15458 | 71.2 | high performance liquid chromatography (HPLC) |
| 29162 | 71.2 |
External links
@ref: 15458
culture collection no.: DSM 45171, CCTCC AA 208048, YIM 91353
straininfo link
- @ref: 80485
- straininfo: 397479
literature
- topic: Phylogeny
- Pubmed-ID: 19622663
- title: Streptomonospora amylolytica sp. nov. and Streptomonospora flavalba sp. nov., two novel halophilic actinomycetes isolated from a salt lake.
- authors: Cai M, Tang SK, Chen YG, Li Y, Zhang YQ, Li WJ
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.007682-0
- year: 2009
- mesh: Actinomycetales/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Water Microbiology
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 15458 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45171) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45171 | |||
| 20173 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM45171.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29162 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25586 | 28776041 | |
| 68382 | Automatically annotated from API zym | |||||
| 80485 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397479.1 | StrainInfo: A central database for resolving microbial strain identifiers |