Strain identifier

BacDive ID: 11007

Type strain: No

Species: Rhodococcus opacus

Strain Designation: MoAcy1

Strain history: <- B. Rosner, Universität Konstanz, MoAcy1

NCBI tax ID(s): 37919 (species)

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General

@ref: 11584

BacDive-ID: 11007

DSM-Number: 44186

keywords: genome sequence, spore-forming, mesophilic

description: Rhodococcus opacus MoAcy1 is a spore-forming, mesophilic prokaryote that was isolated from dry garden soil sample taken near a parking lot.

NCBI tax id

  • NCBI tax id: 37919
  • Matching level: species

strain history

  • @ref: 11584
  • history: <- B. Rosner, Universität Konstanz, MoAcy1

doi: 10.13145/bacdive11007.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • genus: Rhodococcus
  • species: Rhodococcus opacus
  • full scientific name: Rhodococcus opacus Klatte et al. 1995

  • synonyms

    @refsynonym
    20215Rhodococcus imtechensis
    20215Rhodococcus percolatus

@ref: 11584

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Rhodococcus

species: Rhodococcus opacus

full scientific name: Rhodococcus opacus Klatte et al. 1995

strain designation: MoAcy1

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no90.169
69480100positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11584BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b)yeshttps://mediadive.dsmz.de/medium/215bName: BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) Composition: Brain heart infusion 37.0 g/l Glucose 5.0 g/l Distilled water
11584TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

  • @ref: 11584
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes91
69480no98.694

Isolation, sampling and environmental information

isolation

  • @ref: 11584
  • sample type: dry garden soil sample taken near a parking lot
  • geographic location: Tübingen
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Garden
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 11584
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Rhodococcus opacus DSM 44186GCA_019856255completencbi37919
66792Rhodococcus opacus DSM 44186GCA_003626495contigncbi37919
66792Rhodococcus opacus strain DSM 4418637919.28wgspatric37919
66792Rhodococcus opacus strain DSM 4418637919.59completepatric37919
66792Rhodococcus opacus strain DSM 4418637919.73completepatric37919
66792Rhodococcus opacus strain DSM 4418637919.71completepatric37919
66792Rhodococcus opacus strain DSM 4418637919.68completepatric37919

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes91no
flagellatedno97.918no
gram-positiveyes81.524no
anaerobicno99.237no
aerobicyes89.84no
halophileno85.947no
spore-formingno66.364no
motileno92.172no
thermophileno99.537yes
glucose-utilyes90.116no
glucose-fermentno89.573no

External links

@ref: 11584

culture collection no.: DSM 44186

straininfo link

  • @ref: 80261
  • straininfo: 50514

literature

  • topic: Genetics
  • Pubmed-ID: 34989600
  • title: Complete Genome Sequence of Rhodococcus opacus Strain MoAcy1 (DSM 44186), an Aerobic Acetylenotroph Isolated from Soil.
  • authors: Sutton JM, Bushman TJ, Akob DM, Fierst JL
  • journal: Microbiol Resour Announc
  • DOI: 10.1128/MRA.00814-21
  • year: 2022
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitle
11584Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44186)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44186
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80261Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50514.1StrainInfo: A central database for resolving microbial strain identifiers