Strain identifier

BacDive ID: 10895

Type strain: No

Species: Rhodococcus rhodnii

Strain Designation: A/O, Gdf N444, Hill A/O, N 444

Strain history: CIP <- 1995, DSM <- M. Goodfellow: strain N 444 <- P. Hill: strain A/O

NCBI tax ID(s): 38312 (species)

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General

@ref: 11301

BacDive-ID: 10895

DSM-Number: 43959

keywords: 16S sequence, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Rhodococcus rhodnii A/O is an obligate aerobe, mesophilic, Gram-positive prokaryote.

NCBI tax id

  • NCBI tax id: 38312
  • Matching level: species

strain history

@refhistory
11301<- M. Goodfellow, N 444 <- P. Hill, A/O
122222CIP <- 1995, DSM <- M. Goodfellow: strain N 444 <- P. Hill: strain A/O

doi: 10.13145/bacdive10895.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • genus: Rhodococcus
  • species: Rhodococcus rhodnii
  • full scientific name: Rhodococcus rhodnii Goodfellow and Alderson 1979 (Approved Lists 1980)

@ref: 11301

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Rhodococcus

species: Rhodococcus rhodnii

full scientific name: Rhodococcus rhodnii Goodfellow and Alderson 1979 emend. Nouioui et al. 2018

strain designation: A/O, Gdf N444, Hill A/O, N 444

type strain: no

Morphology

cell morphology

  • @ref: 122222
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 122222

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11301BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b)yeshttps://mediadive.dsmz.de/medium/215bName: BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) Composition: Brain heart infusion 37.0 g/l Glucose 5.0 g/l Distilled water
11301TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11301ORGANIC MEDIUM 79 (DSMZ Medium 426)yeshttps://mediadive.dsmz.de/medium/426Name: ORGANIC MEDIUM 79 (DSMZ Medium 426) Composition: Agar 15.0 g/l Peptone 10.0 g/l Dextrose 10.0 g/l NaCl 6.0 g/l Yeast extract 2.0 g/l Casein peptone 2.0 g/l Distilled water
34158MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122222CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
11301positivegrowth28mesophilic
11301positivegrowth30mesophilic
34158positivegrowth30mesophilic
122222positivegrowth25-37mesophilic
122222nogrowth10psychrophilic
122222nogrowth41thermophilic
122222nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122222
  • oxygen tolerance: obligate aerobe

halophily

  • @ref: 122222
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12222216947citrate-carbon source
1222224853esculin-hydrolysis
122222606565hippurate+hydrolysis
12222217632nitrate-reduction
12222216301nitrite-reduction
12222217632nitrate-respiration

antibiotic resistance

  • @ref: 122222
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 122222
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12222215688acetoin-
12222217234glucose-

enzymes

@refvalueactivityec
122222oxidase-
122222beta-galactosidase-3.2.1.23
122222alcohol dehydrogenase-1.1.1.1
122222gelatinase-
122222amylase-
122222DNase-
122222caseinase-3.4.21.50
122222catalase+1.11.1.6
122222tween esterase-
122222gamma-glutamyltransferase-2.3.2.2
122222lecithinase-
122222lipase-
122222lysine decarboxylase-4.1.1.18
122222ornithine decarboxylase-4.1.1.17
122222phenylalanine ammonia-lyase-4.3.1.24
122222tryptophan deaminase-
122222urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122222--+++++---++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122222+-------------------------+------+--+------------+----------+--------------+---++-------+-------++-

Isolation, sampling and environmental information

isolation

  • @ref: 122222
  • isolation date: 1979

taxonmaps

  • @ref: 69479
  • File name: preview.99_4925.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_14;97_1541;98_3688;99_4925&stattab=map
  • Last taxonomy: Rhodococcus rhodnii subclade
  • 16S sequence: X80623
  • Sequence Identity:
  • Total samples: 381
  • soil counts: 172
  • aquatic counts: 89
  • animal counts: 101
  • plant counts: 19

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113011Risk group (German classification)
1222221Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Rhodococcus rhodnii 16S rRNA gene, strain DSM43959
  • accession: X80623
  • length: 1482
  • database: ena
  • NCBI tax ID: 38312

External links

@ref: 11301

culture collection no.: DSM 43959, CIP 104654, IMET 7464

straininfo link

  • @ref: 80150
  • straininfo: 50467

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11301Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43959)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43959
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34158Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16586
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80150Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50467.1StrainInfo: A central database for resolving microbial strain identifiers
122222Curators of the CIPCollection of Institut Pasteur (CIP 104654)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104654