Strain identifier

BacDive ID: 10824

Type strain: Yes

Species: Nocardia iowensis

Strain Designation: BM123, UI122540

Strain history: DSM 45197 <-- J. P. Rosazza Ul 122540 <-- NRRL 5646 <-- Lederle Labs.; BM123.

NCBI tax ID(s): 204891 (species)

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General

@ref: 15691

BacDive-ID: 10824

DSM-Number: 45197

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Nocardia iowensis BM123 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from garden soil.

NCBI tax id

  • NCBI tax id: 204891
  • Matching level: species

strain history

@refhistory
15691<- J. P. Rosazza, University of Iowa; UI122540 <- NRRL <- Lederle Laboratories; BM123
67770DSM 45197 <-- J. P. Rosazza Ul 122540 <-- NRRL 5646 <-- Lederle Labs.; BM123.

doi: 10.13145/bacdive10824.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia iowensis
  • full scientific name: Nocardia iowensis Lamm et al. 2009

@ref: 15691

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Nocardia

species: Nocardia iowensis

full scientific name: Nocardia iowensis Lamm et al. 2009

strain designation: BM123, UI122540

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
29152positiverod-shaped
69480no93.926
69480positive100

colony morphology

@refcolony colormedium used
69377Ivory (1014)ISP 5
69377Mahogany brown (8016)suter with tyrosine
69377Ochar brown (8001)ISP 7
69377Ochre yellow (1024)ISP 2
69377Sand yellow (1002)suter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69377yesAerial myceliumCream (9001)ISP 2
69377yesAerial myceliumCream (9001)ISP 5
69377yesAerial myceliumBeige (1001)ISP 7
69377yesAerial myceliumBasalt grey (7012)suter with tyrosine
69377yesAerial myceliumIvory (1014)suter without tyrosine

pigmentation

@refproductionnamecolor
29152no
69377noMelanin
69377yessoluble pigmentBeige (1001), copper brown (8004)

multimedia

@refmultimedia contentintellectual property rightscaption
15691https://www.dsmz.de/microorganisms/photos/DSM_45197-1.jpg© Leibniz-Institut DSMZ
15691https://www.dsmz.de/microorganisms/photos/DSM_45197.jpg© Leibniz-Institut DSMZMedium 535 28°C
69377DSM_45197_image3.jpegHelmholtz-Zentrum für Infektionsforschung GmbHPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15691GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
15691TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15691positivegrowth28mesophilic
19923positiveoptimum28mesophilic
29152positiveoptimum29mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
29152yes
69481yes100
69480yes98.262

compound production

  • @ref: 67770
  • compound: Antibiotic BM123 (US Pat. 4,007,167 and 4,237,120)

halophily

@refsaltgrowthtested relationconcentration
29152NaClpositivegrowth<7 %
69377NaClpositivegrowth0-5 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4, ω-cycl)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2915228757fructose+carbon source
2915217234glucose+carbon source
2915217306maltose+carbon source
2915217814salicin+carbon source
2915227082trehalose+carbon source
291524853esculin+hydrolysis
2915217632nitrate+reduction
6836927689decanoate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
6937722599arabinose-growth
6937762968cellulose-growth
6937728757fructose-growth
6937717234glucose+growth
6937717268inositol+growth
6937737684mannose-growth
6937716634raffinose-growth
6937726546rhamnose-growth
6937717992sucrose-growth
6937718222xylose-growth
6837917632nitrate+reduction
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
29152catalase+1.11.1.6
29152urease+3.5.1.5
68379gelatinase+
68379urease+3.5.1.5
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382leucine arylamidase+3.4.11.1
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69377+--+--+++/-++--------

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
15691-+---+----++---+++--
69377+++/-+/-+++/-+/--++---++++-

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
15691+----+-+/-++-+/-+-+/--+/-+/-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15691garden soilOsceola, IowaUSAUSANorth America
67770Garden soilOsceola, IAUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

Safety information

risk assessment

  • @ref: 15691
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15691
  • description: Nocardia sp. NRRL 5646 16S ribosomal RNA gene, partial sequence
  • accession: DQ925490
  • length: 1427
  • database: ena
  • NCBI tax ID: 204891

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nocardia iowensis NRRL 5646GCA_019222765completencbi204891
66792Nocardia iowensis strain NRRL 5646204891.4completepatric204891

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno96.841no
gram-positiveyes85.991yes
anaerobicno99.41no
aerobicyes89.846no
halophileno93.814no
spore-formingyes89.593yes
motileno91.628no
thermophileno99.479no
glucose-utilyes88.932yes
glucose-fermentno92.671yes

External links

@ref: 15691

culture collection no.: DSM 45197, JCM 18299, NRRL 5646, NRRL B-24671

straininfo link

  • @ref: 80085
  • straininfo: 406680

literature

  • topic: Phylogeny
  • Pubmed-ID: 19622667
  • title: Nocardia iowensis sp. nov., an organism rich in biocatalytically important enzymes and nitric oxide synthase.
  • authors: Lamm AS, Khare A, Conville P, Lau PC, Bergeron H, Rosazza JP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.007427-0
  • year: 2009
  • mesh: Bacterial Proteins/*genetics/metabolism, Biocatalysis, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Nitric Oxide Synthase/*genetics/metabolism, Nocardia/classification/*enzymology/genetics/*isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15691Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45197)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45197
19923Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45197.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29152Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2557628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69377Wink, J.https://cdn.dsmz.de/wink/DSM%2045197.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80085Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406680.1StrainInfo: A central database for resolving microbial strain identifiers