Strain identifier
BacDive ID: 10536
Type strain: 
Species: Leptospirillum ferriphilum
Strain Designation: P3A, P3A
Strain history: <- N. J. Coram, University of Stellenbosch, Department of Microbiology, Matieland, South Africa; ATCC 49881 <- ATCC <- W. Sand, University Hamburg, Dept. Mikrobiologie, Hamburg, Germany; P3A
NCBI tax ID(s): 178606 (species)
version 8.1 (current version)
General
@ref: 5461
BacDive-ID: 10536
DSM-Number: 14647
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Leptospirillum ferriphilum P3A is a mesophilic bacterium that was isolated from enrichment culture.
NCBI tax id
- NCBI tax id: 178606
- Matching level: species
strain history
- @ref: 5461
- history: <- N. J. Coram, University of Stellenbosch, Department of Microbiology, Matieland, South Africa; ATCC 49881 <- ATCC <- W. Sand, University Hamburg, Dept. Mikrobiologie, Hamburg, Germany; P3A
doi: 10.13145/bacdive10536.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/nitrospirota
- domain: Bacteria
- phylum: Nitrospirota
- class: Nitrospiria
- order: Nitrospirales
- family: Nitrospiraceae
- genus: Leptospirillum
- species: Leptospirillum ferriphilum
- full scientific name: Leptospirillum ferriphilum Coram and Rawlings 2002
@ref: 5461
domain: Bacteria
phylum: Nitrospirae
class: Nitrospira
order: Nitrospirales, not validated
family: Nitrospiraceae
genus: Leptospirillum
species: Leptospirillum ferriphilum
full scientific name: Leptospirillum ferriphilum Coram and Rawlings 2002
strain designation: P3A, P3A
type strain: yes
Morphology
colony morphology
- @ref: 5461
- incubation period: 8-14 days
Culture and growth conditions
culture medium
- @ref: 5461
- name: LEPTOSPIRILLUM (HH) MEDIUM (DSMZ Medium 882)
- growth: yes
- link: https://mediadive.dsmz.de/medium/882
- composition: Name: LEPTOSPIRILLUM (HH) MEDIUM (DSMZ Medium 882) Composition: FeSO4 x 7 H2O 19.98 g/l CaCl2 x 2 H2O 0.146853 g/l (NH4)2SO4 0.131868 g/l MgCl2 x 6 H2O 0.0529471 g/l KH2PO4 0.0269731 g/l MnCl2 x 4 H2O 7.59241e-05 g/l ZnCl2 6.79321e-05 g/l CuCl2 x 2 H2O 6.69331e-05 g/l CoCl2 x 6 H2O 6.39361e-05 g/l H3BO3 3.0969e-05 g/l Na2MoO4 9.99001e-06 g/l H2SO4 Distilled water
culture temp
- @ref: 5461
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Isolation, sampling and environmental information
isolation
- @ref: 5461
- sample type: enrichment culture
- geographic location: Lima
- country: Peru
- origin.country: PER
- continent: Middle and South America
isolation source categories
- Cat1: #Engineered
- Cat2: #Laboratory
- Cat3: #Lab enrichment
taxonmaps
- @ref: 69479
- File name: preview.99_10283.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_251;96_987;97_1148;98_1371;99_10283&stattab=map
- Last taxonomy: Leptospirillum ferriphilum subclade
- 16S sequence: AF356829
- Sequence Identity:
- Total samples: 142
- soil counts: 17
- aquatic counts: 109
- animal counts: 12
- plant counts: 4
Safety information
risk assessment
- @ref: 5461
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5461
- description: Leptospirillum ferriphilum strain ATCC 49881 16S ribosomal RNA gene, partial sequence
- accession: AF356829
- length: 1516
- database: ena
- NCBI tax ID: 178606
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Leptospirillum ferriphilum DSM 14647 | GCA_000755505 | contig | ncbi | 178606 |
| 66792 | Leptospirillum ferriphilum strain DSM 14647 | 178606.4 | wgs | patric | 178606 |
| 66792 | Leptospirillum ferriphilum DSM 14647 | 2609460196 | draft | img | 178606 |
GC content
- @ref: 5461
- GC-content: 56.6
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 85.244 | no |
| gram-positive | no | 98.82 | no |
| anaerobic | no | 68.554 | no |
| aerobic | no | 81.227 | no |
| halophile | no | 92.327 | no |
| spore-forming | no | 88.606 | no |
| thermophile | yes | 70.464 | yes |
| glucose-util | yes | 53.49 | no |
| flagellated | no | 86.471 | no |
| glucose-ferment | no | 82.415 | no |
External links
@ref: 5461
culture collection no.: DSM 14647, ATCC 49881
straininfo link
- @ref: 79812
- straininfo: 87760
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 22970253 | Thiol/Disulfide system plays a crucial role in redox protection in the acidophilic iron-oxidizing bacterium Leptospirillum ferriphilum. | Norambuena J, Flores R, Cardenas JP, Quatrini R, Chavez R, Levican G | PLoS One | 10.1371/journal.pone.0044576 | 2012 | Bacteria/genetics/*metabolism, Base Sequence, DNA Primers, Disulfides/*metabolism, Genes, Bacterial, Glutathione Reductase/metabolism, Insulin/metabolism, Iron/*metabolism, Oxidation-Reduction, Polymerase Chain Reaction, Sulfhydryl Compounds/*metabolism | Enzymology |
| Genetics | 25377718 | Draft Genome Sequence of the Iron-Oxidizing Acidophile Leptospirillum ferriphilum Type Strain DSM 14647. | Cardenas JP, Lazcano M, Ossandon FJ, Corbett M, Holmes DS, Watkin E | Genome Announc | 10.1128/genomeA.01153-14 | 2014 | ||
| Metabolism | 29150517 | Multi-omics Reveals the Lifestyle of the Acidophilic, Mineral-Oxidizing Model Species Leptospirillum ferriphilum(T). | Christel S, Herold M, Bellenberg S, El Hajjami M, Buetti-Dinh A, Pivkin IV, Sand W, Wilmes P, Poetsch A, Dopson M | Appl Environ Microbiol | 10.1128/AEM.02091-17 | 2018 | Bacteria/classification/*genetics/metabolism, Copper/metabolism, *Genome, Bacterial, Hydrogen-Ion Concentration, Iron/*metabolism, Oxidation-Reduction, Phylogeny, *Proteome, Proteomics, RNA, Bacterial/*genetics/metabolism, *Transcriptome | Phylogeny |
| Stress | 31736901 | Osmotic Imbalance, Cytoplasm Acidification and Oxidative Stress Induction Support the High Toxicity of Chloride in Acidophilic Bacteria. | Rivera-Araya J, Pollender A, Huynh D, Schlomann M, Chavez R, Levican G | Front Microbiol | 10.3389/fmicb.2019.02455 | 2019 | ||
| Transcriptome | 35486621 | Transcriptomic analysis of chloride tolerance in Leptospirillum ferriphilum DSM 14647 adapted to NaCl. | Rivera-Araya J, Heine T, Chavez R, Schlomann M, Levican G | PLoS One | 10.1371/journal.pone.0267316 | 2022 | Bacteria/genetics, *Chlorides, Halogens, Reactive Oxygen Species, *Sodium Chloride, Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5461 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14647) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14647 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 79812 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID87760.1 | StrainInfo: A central database for resolving microbial strain identifiers |