Strain identifier

BacDive ID: 10490

Type strain: Yes

Species: Neisseria polysaccharea

Strain Designation: LNP N 462

Strain history: CIP <- 1983, J.Y. Riou, Inst. Pasteur, Paris, France: strain LNP N 462

NCBI tax ID(s): 546267 (strain), 489 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 16528

BacDive-ID: 10490

DSM-Number: 22809

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, coccus-shaped

description: Neisseria polysaccharea LNP N 462 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from healthy child, throat.

NCBI tax id

NCBI tax idMatching level
489species
546267strain

strain history

@refhistory
16528<- CIP; CIP 100113 <- J.-Y. Riou, CIP; LNP N 462
379541983, J.Y. Riou, Inst. Pasteur, Paris, France: strain LNP N 462
121934CIP <- 1983, J.Y. Riou, Inst. Pasteur, Paris, France: strain LNP N 462

doi: 10.13145/bacdive10490.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria polysaccharea
  • full scientific name: Neisseria polysaccharea Riou and Guibourdenche 1987

@ref: 16528

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria polysaccharea

full scientific name: Neisseria polysaccharea Riou and Guibourdenche 1987

strain designation: LNP N 462

type strain: yes

Morphology

cell morphology

  • @ref: 121934
  • gram stain: negative
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 121934

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16528COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
16528COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37954MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121934CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
16528positivegrowth37mesophilic
37954positivegrowth30mesophilic
46740positivegrowth37mesophilic
121934positivegrowth30-37mesophilic
121934nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16528microaerophile
46740microaerophile
121934obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12193417632nitrate-reduction
12193416301nitrite+reduction
12193435020tributyrin-hydrolysis
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

antibiotic resistance

  • @ref: 121934
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12193435581indoleno
121934polysaccharidesno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
16528catalase+1.11.1.6
16528cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
121934oxidase+
121934beta-galactosidase-3.2.1.23
121934alcohol dehydrogenase-1.1.1.1
121934DNase-
121934catalase+1.11.1.6
121934gamma-glutamyltransferase+2.3.2.2
121934lysine decarboxylase-4.1.1.18
121934ornithine decarboxylase-4.1.1.17
121934urease-3.5.1.5

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16528+-++/------+---
16528+-++-----+--
16528-+-++/------+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
16528healthy child, throatFranceFRAEurope
46740Human throat,healthy childFranceFRAEurope1975
121934Human, Throat, healthy childFranceFRAEurope1975

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body-Site#Oral cavity and airways#Throat
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_119.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_42;97_98;98_107;99_119&stattab=map
  • Last taxonomy: Neisseria
  • 16S sequence: AJ239289
  • Sequence Identity:
  • Total samples: 31491
  • soil counts: 653
  • aquatic counts: 2713
  • animal counts: 27799
  • plant counts: 326

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
165281Risk group (German classification)
1219341Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Neisseria polysaccharea 16S ribosomal RNAL061671479ena489
20218Neisseria polysaccharea ATCC 43768 16S rRNA gene, partial sequenceU02902392ena489
16528Neisseria polysaccharea 16S rRNA gene (partial), strain NCTC11858AJ2392891355ena489

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria polysaccharea ATCC 43768GCA_000176735contigncbi546267
66792Neisseria polysaccharea ATCC 43768546267.4wgspatric546267
66792Neisseria polysaccharea ATCC 43768647000284draftimg546267

GC content

  • @ref: 16528
  • GC-content: 55.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.579no
anaerobicno96.353yes
halophileno63.03no
spore-formingno97.133no
glucose-utilyes60.817no
thermophileno98.699yes
aerobicno95.941no
motileno85.94no
flagellatedno90.74no
glucose-fermentno88.839no

External links

@ref: 16528

culture collection no.: DSM 22809, ATCC 43768, CCUG 18030, CIP 100113, NCTC 11858

straininfo link

  • @ref: 79767
  • straininfo: 42026

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism3815160Structure of the exocellular D-glucan produced by Neisseria polysaccharea.Riou JY, Guibourdenche M, Perry MB, MacLean LL, Griffith DWCan J Microbiol10.1139/m86-1671986Centrifugation, Chemical Phenomena, Chemistry, Chromatography, Gel, Chromatography, Paper, Glucans/*analysis/biosynthesis, Magnetic Resonance Spectroscopy, Neisseria/*metabolism
Enzymology9150231Cloning and characterization of the gene for amylosucrase from Neisseria polysaccharea: production of a linear alpha-1,4-glucan.Buttcher V, Welsh T, Willmitzer L, Kossmann JJ Bacteriol10.1128/jb.179.10.3324-3330.19971997Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli/enzymology/genetics, Extracellular Space/enzymology/microbiology, *Genes, Bacterial, Glucans/*biosynthesis/chemistry, Glucosyltransferases/chemistry/*genetics/isolation & purification, Molecular Sequence Data, Neisseria/*enzymology/*genetics/metabolism, Protein Sorting Signals/chemistry, Sequence Homology, Amino AcidGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16528Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22809)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22809
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37954Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11542
46740Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18030)https://www.ccug.se/strain?id=18030
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
79767Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42026.1StrainInfo: A central database for resolving microbial strain identifiers
121934Curators of the CIPCollection of Institut Pasteur (CIP 100113)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20100113