Name: Helicobacter pylori T81213-NTB
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6099

Type strain:

Species: Helicobacter pylori

Strain Designation: T81213-NTB, T81123-NTB

Culture col. no.: DSM 7492, ATCC 49396, JCM 12096, CCUG 45423, CIP 104754, LMG 14378, NCTC 12491, CCUG 32350, CCUG 44615

Strain history: CIP <- 1997, CCUG, Helicobacter nemestrinae <- 1993, ATCC <- M.A. Bronsdon: strain T81123-NTB

NCBI tax ID(s): 210 (species)

SI-ID 7930

LMG 14378, ATCC 49396, CCUG 32350, CCUG 45423, CIP 104754, CCUG 44615, DSM 7492, NCTC 12491, JCM 12096

Other strains from Helicobacter pylori

Section

Helicobacter pylori T81213-NTB is a mesophilic, Gram-negative, motile bacterium that was isolated from gastric mucosa of pigtailed macaque.

Gram-negative
motile
rod-shaped
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Helicobacteraceae*
	Genus	*Helicobacter*
	Species	**Helicobacter pylori**
	Full Scientific Name (LPSN)	Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989

	Synonym	Campylobacter pyloridis
	Synonym	Helicobacter nemestrinae
	Synonym	Campylobacter pylori

Information on morphological and physiological properties Morphology

[Ref.: #122083]	Gram stain	negative

[Ref.: #122083]	Cell shape	rod-shaped

[Ref.: #122083]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3190]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3190]

Culture medium growth

[Ref.: #3190]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3190]

Culture medium composition

expand / minimize

[Ref.: #39088]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #39088]

Culture medium growth

[Ref.: #39088]

Culture medium composition

expand / minimize

[Ref.: #122083]

Culture medium

Brucella broth

[Ref.: #122083]

Culture medium growth

[Ref.: #122083]

Culture medium

CIP Medium 6

[Ref.: #122083]

Culture medium growth

[Ref.: #122083]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3190]		growth	37 °C
[Ref.: #39088]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #122083]		growth	30-41 °C
[Ref.: #122083]	no	growth	5 °C
[Ref.: #122083]	no	growth	10 °C
[Ref.: #122083]	no	growth	25 °C
[Ref.: #122083]	no	growth	45 °C

[Ref.: #3190]	Temperature range	mesophilic
[Ref.: #39088]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #122083]	Temperature range	psychrophilic
[Ref.: #122083]	Temperature range	mesophilic
[Ref.: #122083]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #122083]		NaCl	no	growth	3.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	D-glucose ChEBI	-	assimilation	* from API CAM
[Ref.: #122083]	hippurate ChEBI	-	hydrolysis
[Ref.: #68373]	hippurate ChEBI	-	hydrolysis	* from API CAM
[Ref.: #68373]	malate ChEBI	-	assimilation	* from API CAM
[Ref.: #122083]	nitrate ChEBI	-	reduction
[Ref.: #68373]	nitrate ChEBI	-	reduction	* from API CAM
[Ref.: #122083]	nitrite ChEBI	-	reduction
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	urea ChEBI	+	hydrolysis	* from API CAM
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance
[Ref.: #122083]	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no			yes
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg		* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg		* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg		* from API CAM

	Metabolite production	Metabolite	Production
[Ref.: #122083]		indole ChEBI	no
[Ref.: #68373]		hydrogen sulfide ChEBI	no	* from API CAM

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API CAM
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122083]	catalase	+	1.11.1.6
[Ref.: #68373]	catalase	+	1.11.1.6	* from API CAM
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122083]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #68373]	gamma-glutamyltransferase	+	2.3.2.2	* from API CAM
[Ref.: #122083]	gelatinase	-
[Ref.: #68373]	L-aspartate arylamidase	-	3.4.11.21	* from API CAM
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122083]	oxidase	+
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122083]	urease	+	3.5.1.5
[Ref.: #68373]	urease	+	3.5.1.5	* from API CAM
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #3190]	3947	+	-	-	-	+	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	+
[Ref.: #3190]	3839	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3190]	3725	+	-	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3190]	3724	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3190]	3722	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3190]	3721	+	-	+	-	+	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	+
[Ref.: #3190]	3720	+	-	+/-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122083]	8173	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3190]	Sample type/isolated from	gastric mucosa of pigtailed macaque
[Ref.: #3190]	Geographic location (country and/or sea, region)	Seattle
[Ref.: #3190]	Country	USA
[Ref.: #3190]	Country ISO 3 Code	USA
[Ref.: #3190]	Continent	North America
[Ref.: #3190]	Geographic location	47.6062°/-122.3320°

[Ref.: #67770]	Sample type/isolated from	Gastric mucosa of a 6-year-old healthy pigtailed macaque (Macaca nemestrina)
[Ref.: #67770]	Host species	Macaca nemestrina

[Ref.: #122083]	Sample type/isolated from	Animal, Pig - tailed macaque, gastric mucosa
[Ref.: #122083]	Country	United States of America
[Ref.: #122083]	Country ISO 3 Code	USA
[Ref.: #122083]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Primates
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC507449 (>99% sequence identity) for Helicobacter pylori subclade from Microbeatlas

Aquatic Samples	829
Soil Samples	670
Animal Samples	16307
Plant Samples	278
Total Samples	18084

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3190]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122083]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Helicobacter pylori JCM 12096 gene for 16S ribosomal RNA, partial sequence	LC507449	1426	ENA	210 tax ID
[Ref.: #20218]	H.nemestrinae (ATCC 49396T) gene for 16S rRNA	X67854	1463	ENA	210 tax ID	*
[Ref.: #20218]	Helicobacter nemestrinae 16S ribosomal RNA gene, partial sequence	AF348617	1477	ENA	210 tax ID	*
[Ref.: #20218]	Helicobacter nemestrinae strain ATCC49396 16S ribosomal RNA gene, partial sequence	AF363064	1437	ENA	210 tax ID	*

[Ref.: #3190]	GC-content	24.0 mol%
[Ref.: #67770]	GC-content	24 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #3190]

Culture collection no.

DSM 7492, ATCC 49396, JCM 12096, CCUG 45423, CIP 104754, LMG 14378, NCTC 12491, CCUG 32350, CCUG 44615

[Ref.: #75587]

SI-ID 7930

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection.	Horiuchi S, Nakano R, Nakano A, Hishiya N, Uno K, Suzuki Y, Tanouchi A, Kakuta N, Masui T, Jojima N, Yano H	J Microbiol Methods	10.1016/j.mimet.2019.105653	2019	*
Phylogeny	Helicobacter nemestrinae ATCC 49396T is a strain of Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989, and Helicobacter nemestrinae Bronsdon et al. 1991 is therefore a junior heterotypic synonym of Helicobacter pylori.	Suerbaum S, Kraft C, Dewhirst FE, Fox JG	Int J Syst Evol Microbiol	10.1099/00207713-52-2-437	2002	*
Phylogeny	Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina)	Bronsdon MA, Goodwin CS, Sly LI, Chilvers T, Schoenknecht FD	Int J Syst Bacteriol	10.1099/00207713-41-1-148	1991	*

References

#3190

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7492

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39088 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68373

Automatically annotated from API CAM .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#75587

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7930.1 )

#122083 Collection of Institut Pasteur ; Curators of the CIP; CIP 104754
* These data were automatically processed and therefore are not curated

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