Name: Acholeplasma laidlawii PG 8
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 97

Type strain:

Species: Acholeplasma laidlawii

Strain Designation: PG 8, PG8

Culture col. no.: DSM 23060, ATCC 23206, CIP 75.27, IFO (now NBRC) 14400, NCTC 10116, CCUG 32754

Strain history: CIP <- 1975, ATCC <- E.A. Freundt: strain PG8 <- Nat. Inst. Med. Res. A <- P.P. Laidlaw and W.J. Elford

NCBI tax ID(s): 2148 (species)

SI-ID 37937

ATCC 23206, NCTC 10116, CCUG 32754, IFO 14400, IMET 10894, NBRC 14400, CIP 75.27, DSM 23060

Special Collections

Other strains from Acholeplasma laidlawii

Section

Acholeplasma laidlawii PG 8 is a mesophilic, Gram-negative animal pathogen that was isolated from sewage.

Gram-negative
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Acholeplasmatales*
	Family	*Acholeplasmataceae*
	Genus	*Acholeplasma*
	Species	**Acholeplasma laidlawii**
	Full Scientific Name (LPSN)	Acholeplasma laidlawii (Sabin 1941) Edward and Freundt 1970 (Approved Lists 1980)

Synonym

"Sapromyces laidlawi"

Information on morphological and physiological properties Morphology

[Ref.: #120550]	Gram stain	negative

[Ref.: #69480]	Motility	no Confidence in %93

[Ref.: #16683]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16683]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

[Ref.: #16683]

Culture medium growth

[Ref.: #16683]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16683]

Culture medium composition

expand / minimize

[Ref.: #32774]

Culture medium

MEDIUM 88 - for Mycoplasma and Acholeplasma

[Ref.: #32774]

Culture medium growth

[Ref.: #32774]

Culture medium composition

expand / minimize

[Ref.: #120550]

Culture medium

CIP Medium 88

[Ref.: #120550]

Culture medium growth

[Ref.: #120550]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120550]

Culture medium

CIP Medium 749

[Ref.: #120550]

Culture medium growth

[Ref.: #120550]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #16683]			growth	37 °C
[Ref.: #32774]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16683]	Sample type/isolated from	sewage

[Ref.: #120550]	Sample type/isolated from	Environment, Sewage
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence U14905 (>99% sequence identity) for Acholeplasma laidlawii subclade from Microbeatlas

Aquatic Samples	5
Soil Samples
Animal Samples	16
Plant Samples	1
Total Samples	22

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16683]	Pathogenicity (animal)	yes
[Ref.: #16683]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120550]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Acholeplasma laidlawii PG8 ATCC 23206 16S ribosomal RNA gene, partial sequence	U14905	1476	GenBank ENA	2148 tax ID	*
[Ref.: #20218]	Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY740436	769	GenBank ENA	2148 tax ID	*
[Ref.: #20218]	Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY740437	978	GenBank ENA	2148 tax ID	*
[Ref.: #20218]	Acholeplasma laidlawii 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF294996	664	GenBank ENA	2148 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acholeplasma laidlawii DSM 23060	GCA_003385765	scaffold	GenBank ENA	2148 tax ID	*
[Ref.: #66792]	Acholeplasma laidlawii NCTC10116	GCA_900476025	complete	GenBank ENA	2148 tax ID	*
[Ref.: #66792]	Acholeplasma laidlawii strain DSM 23060	2148.14	wgs	PATRIC	2148 tax ID	*
[Ref.: #66792]	Acholeplasma laidlawii strain NCTC10116	2148.13	complete	PATRIC	2148 tax ID	*
[Ref.: #66792]	Acholeplasma laidlawii DSM 23060	2757320367	draft	JGI IMG/M	2148 tax ID	*
[Ref.: #66792]	Acholeplasma laidlawii NCTC 10116	2826166747	complete	JGI IMG/M	2148 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Acholeplasma laidlawii strain NCTC10116 PATRIC: 2148.13
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Acholeplasma laidlawii NCTC10116 NCBI: GCA_900476025.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	64.384	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	77.747	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	90.005	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	82.57	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.996	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93	no

Availability in culture collections External links

[Ref.: #16683]

Culture collection no.

DSM 23060, ATCC 23206, CIP 75.27, IFO (now NBRC) 14400, NCTC 10116, CCUG 32754

[Ref.: #69780]

SI-ID 37937

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Sensitive and selective detection of mycoplasma in cell culture samples using cantilever sensors.	Xu S, Sharma H, Mutharasan R	Biotechnol Bioeng	10.1002/bit.22637	2010	*
Metabolism	[The phenotypic traits of Mollicutes as their possible phylogenetic markers].	Skrypal' IH, Onyshchenko AM, Malynovs'ka LP, Tokovenko IP, Havrylko LO	Mikrobiol Z		1995	*
Phylogeny	Possible monosaccharide composition of glycopolymers located on the external side of membranes of cells of some mollicutes.	Skripal' IG, Onishchenko AM, Gavrilko LO	Mikrobiol Z		1995	*
Enzymology	Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis.	Robertson JA, Pyle LE, Stemke GW, Finch LR	Nucleic Acids Res	10.1093/nar/18.6.1451	1990	*
Enzymology	[Characterization of Acholeplasma Strains by Horizontal Polyacrylamide Flat Gel Electrophoresis (author's transl)].	Boden K, Kirchhoff H	Zentralbl Bakteriol Orig A		1977	*
	Colony morphology, ultrastructure and morphogenesis in Mycoplasma hominis, Acholeplasma laidlawii and Ureaplasma urealyticum.	Meloni GA, Bertoloni G, Busolo F, Conventi L	J Gen Microbiol	10.1099/00221287-116-2-435	1980	*

References

#16683

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23060

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32774 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69780

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID37937.1 )

#120550 Collection of Institut Pasteur ; Curators of the CIP; CIP 75.27
* These data were automatically processed and therefore are not curated

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Acholeplasma laidlawii strain NCTC10116

Acholeplasma laidlawii NCTC10116

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