Name: Komagataeibacter intermedius TF2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 92

Type strain:

Species: Komagataeibacter intermedius

Strain Designation: TF2

Culture col. no.: DSM 11804, BCC 36457, JCM 16936, LMG 18909, BCC 36447, BCRC 17055, CIP 105780, ZIM B073

Strain history: CIP <- 1998, ZIM <- C. Boesch, Zürich, Switzerland: strain TF2

NCBI tax ID(s): 66229 (species)

SI-ID 12785

LMG 18909, DSM 11804, CIP 105780, BCRC 17055, CCRC 17055, BCC 36447, JCM 16936, BCC 36457, TBRC 477

Special Collections

Other strains from Komagataeibacter intermedius

Section

When using BacDive for research please cite our paper

Komagataeibacter intermedius TF2 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from tea fungus beverage .

Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Komagataeibacter*
	Species	**Komagataeibacter intermedius**
	Full Scientific Name (LPSN)	Komagataeibacter intermedius (Boesch et al. 1998) Yamada et al. 2013

	Synonym	"Komagatabacter* intermedius"*
	Synonym	Acetobacter intermedius
	Synonym	Gluconacetobacter intermedius

Information on morphological and physiological properties Morphology

[Ref.: #120151]	Gram stain	negative
[Ref.: #125439]	Gram stain	negative Confidence in %98.6
[Ref.: #125438]	Gram stain	negative Confidence in %96.833

[Ref.: #120151]	Cell shape	rod-shaped

[Ref.: #120151]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4493]

Culture medium

ALTERNATIVE ACETOBACTER INTERMEDIUS MEDIUM (DSMZ Medium 850)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

AE MEDIUM (DSMZ Medium 849)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

MRS MEDIUM (DSMZ Medium 11)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #4493]

Culture medium composition

expand / minimize

[Ref.: #39826]

Culture medium

MEDIUM 289 - for Acetobacter intermedius

[Ref.: #39826]

Culture medium growth

[Ref.: #39826]

Culture medium composition

expand / minimize

[Ref.: #120151]

Culture medium

CIP Medium 289

[Ref.: #120151]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4493]		growth	30 °C
[Ref.: #39826]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120151]		growth	22-30 °C
[Ref.: #120151]	negative	growth	37 °C
[Ref.: #120151]	negative	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #125439]

Ability of spore formation

no Confidence in %98.5

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120151]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120151]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #120151]	beta-galactosidase	-	3.2.1.23
[Ref.: #120151]	catalase	-	1.11.1.6
[Ref.: #120151]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #120151]	gelatinase	-
[Ref.: #120151]	oxidase	-

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120151]	10230	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4493]	Sample type/isolated from	tea fungus beverage (Komucha)
[Ref.: #4493]	Country	Switzerland
[Ref.: #4493]	Country ISO 3 Code	CHE
[Ref.: #4493]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Liquid phase of a tea fungus beverage (Kombucha)

[Ref.: #120151]	Sample type/isolated from	Swiss Kombucha beverage
[Ref.: #120151]	Geographic location (country and/or sea, region)	Zurich
[Ref.: #120151]	Country	Switzerland
[Ref.: #120151]	Country ISO 3 Code	CHE
[Ref.: #120151]	Continent	Europe
[Ref.: #120151]	Isolation date	1996
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Fermented
#Host	#Fungi	-
#Engineered	#Food production	#Beverage

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Y14694 (>99% sequence identity) for Komagataeibacter from Microbeatlas

Aquatic Samples	523
Soil Samples	662
Animal Samples	2327
Plant Samples	555
Total Samples	4067

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4493]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120151]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4493]	Acetobacter intermedius 16S rRNA gene	Y14694	1481	GenBank ENA	66229 tax ID
[Ref.: #20218]	Gluconacetobacter intermedius strain LMG 18909T 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence	KC478452	747	GenBank ENA	66229 tax ID	*
[Ref.: #20218]	Gluconacetobacter intermedius strain DSM 11804T 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence	KC478454	749	GenBank ENA	66229 tax ID	*
[Ref.: #20218]	Gluconacetobacter intermedius strain LMG 18909 16S ribosomal RNA gene, partial sequence	JF793990	1351	GenBank ENA	66229 tax ID	*
[Ref.: #20218]	Gluconacetobacter intermedius strain DSM 11804 16S ribosomal RNA gene, partial sequence	JF793991	1352	GenBank ENA	66229 tax ID	*
[Ref.: #124043]	Komagataeibacter intermedius partial 16S rRNA gene, strain LMG 18909	OX424603	1485	GenBank ENA	66229 tax ID	*
[Ref.: #20218]	Gluconacetobacter intermedius gene for 16S rRNA, partial sequence, strain: JCM 16936	AB645730	1411	GenBank ENA	66229 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Komagataeibacter intermedius TF2	GCA_000964425	scaffold	GenBank ENA	1231353 tax ID
[Ref.: #66792]	Komagataeibacter intermedius TF2	1231353.3	wgs	PATRIC	1231353 tax ID	*

[Ref.: #67770]

GC-content

61.6 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Komagataeibacter intermedius TF2 PATRIC: 1231353.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	96.833	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	88.176	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	86.514	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	75.786	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	97.751	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	57.636	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Komagataeibacter intermedius TF2 NCBI: GCA_000964425
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	98.5
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	75.3
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	98.6
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	aerobe	86.9

Availability in culture collections External links

[Ref.: #4493]

Culture collection no.

DSM 11804, BCC 36457, JCM 16936, LMG 18909, BCC 36447, BCRC 17055, CIP 105780, ZIM B073

[Ref.: #69775]

SI-ID 12785

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acetobacter intermedius, sp. nov.	Boesch C, Trcek J, Sievers M, Teuber M	Syst Appl Microbiol	10.1016/S0723-2020(98)80026-X	1998	*

References

#4493

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11804

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39826 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69775

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12785.1 )

#120151 Collection of Institut Pasteur ; Curators of the CIP; CIP 105780

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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