Name: Mycobacterium marseillense 5356591
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 8582

Type strain:

Species: Mycobacterium marseillense

Strain Designation: 5356591

Culture col. no.: JCM 17324, DSM 45437, CCUG 56325, CIP 109828, CSUR P30

Strain history: CIP <- 2008, M. Drancourt, Timone Hosp., Marseille, France: strain 5356591

NCBI tax ID(s): 701042 (species)

SI-ID 364722

CCUG 56325, CIP 109828, JCM 17324

Special Collections

Other strains from Mycobacterium marseillense

Section

Mycobacterium marseillense 5356591 is an obligate aerobe, Gram-positive bacterium that was isolated from human sputum.

Gram-positive
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium marseillense**
	Full Scientific Name (LPSN)	Mycobacterium marseillense Ben Salah et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29309]	Gram stain	positive
[Ref.: #120843]	Gram stain	positive

[Ref.: #29309]	Cell length	0.98 µm

[Ref.: #29309]	Cell width	0.52 µm

[Ref.: #120843]	Cell shape	rod-shaped

[Ref.: #120843]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90

[Ref.: #29309]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16815]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #16815]

Culture medium growth

[Ref.: #16815]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16815]

Culture medium composition

expand / minimize

[Ref.: #41748]

Culture medium

MEDIUM 93 - for Mycobacterium avium subsp. sylvaticum

[Ref.: #41748]

Culture medium growth

[Ref.: #41748]

Culture medium composition

expand / minimize

[Ref.: #120843]

Culture medium

CIP Medium 93

[Ref.: #120843]

Culture medium growth

[Ref.: #120843]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16815]	growth	28 °C
[Ref.: #41748]	growth	30 °C
[Ref.: #67770]	growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #120843]

Oxygen tolerance

obligate aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29309]	esculin ChEBI	+	hydrolysis
[Ref.: #120843]	nitrate ChEBI	-	reduction
[Ref.: #120843]	nitrite ChEBI	-	reduction
[Ref.: #29309]	ribose ChEBI	+	carbon source
[Ref.: #29309]	tween 80 ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #120843]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #29309]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #29309]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120843]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120843]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120843]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120843]	12140	-	+	-	+	-	+	-	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16815]	Sample type/isolated from	human sputum
[Ref.: #16815]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #16815]	Country	France
[Ref.: #16815]	Country ISO 3 Code	FRA
[Ref.: #16815]	Continent	Europe

[Ref.: #60919]	Sample type/isolated from	Human sputum
[Ref.: #60919]	Sampling date	2000
[Ref.: #60919]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #60919]	Country	France
[Ref.: #60919]	Country ISO 3 Code	FRA
[Ref.: #60919]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Sputum

[Ref.: #120843]	Sample type/isolated from	Human, Spit
[Ref.: #120843]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #120843]	Country	France
[Ref.: #120843]	Country ISO 3 Code	FRA
[Ref.: #120843]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU266632 (>99% sequence identity) for Mycobacterium from Microbeatlas

Aquatic Samples	4789
Soil Samples	12779
Animal Samples	3677
Plant Samples	2833
Total Samples	24078

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16815]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120843]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16815]	Mycobacterium marseillense strain 5356591 16S ribosomal RNA gene, partial sequence	EU266632	1440	GenBank ENA	701042 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacterium marseillense DSM 45437	GCA_002086345	contig	GenBank ENA	701042 tax ID
[Ref.: #66792]	Mycobacterium marseillense JCM 17324	GCA_010731675	complete	GenBank ENA	701042 tax ID	*
[Ref.: #66792]	Mycobacterium marseillense strain DSM 45437	701042.3	wgs	PATRIC	701042 tax ID	*
[Ref.: #66792]	Mycobacterium marseillense strain JCM 17324	701042.13	wgs	PATRIC	701042 tax ID	*
[Ref.: #66792]	Mycobacterium marseillense DSM 45437	GCA_025823125	contig	GenBank ENA	701042 tax ID	*

[Ref.: #67770]

GC-content

67.7 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium marseillense JCM 17324 NCBI: GCA_010731675.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.824	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	95.84	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	76.998	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	64.259	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90	no

Availability in culture collections External links

[Ref.: #16815]

Culture collection no.

JCM 17324, DSM 45437, CCUG 56325, CIP 109828, CSUR P30

[Ref.: #77966]

SI-ID 364722

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycobacterium marseillense sp. nov., Mycobacterium timonense sp. nov. and Mycobacterium bouchedurhonense sp. nov., members of the Mycobacterium avium complex.	Ben Salah I, Cayrou C, Raoult D, Drancourt M	Int J Syst Evol Microbiol	10.1099/ijs.0.010637-0	2009	*

References

#16815

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45437

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29309

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25722 (see below)

#41748 ; Curators of the CIP;
#60919 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56325

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77966

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID364722.1 )

#120843 Collection of Institut Pasteur ; Curators of the CIP; CIP 109828

#25722

IJSEM 2803 2009 ( DOI 10.1099/ijs.0.010637-0 , PubMed 19628609 )

* These data were automatically processed and therefore are not curated

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Mycobacterium marseillense JCM 17324

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