Name: Mycobacterium vulneris NLA 000700772
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8581

Type strain:

Species: Mycobacterium vulneris

Strain Designation: NLA 000700772, NLA000700772

Culture col. no.: DSM 45247, CIP 109859, CCUG 58459, JCM 18115

Strain history: CIP <- 2008, RIVM <- J. Van Ingen, RIVM, Bilthoven, The Netherlands: strain NLA000700772

NCBI tax ID(s): 547163 (species)

SI-ID 364160

CIP 109859, DSM 45247, JCM 18115

Section

Mycobacterium vulneris NLA 000700772 is a microaerophile, mesophilic, rod-shaped bacterium that was isolated from human, infected wound after dogbite.

rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium vulneris**
	Full Scientific Name (LPSN)	Mycobacterium vulneris van Ingen et al. 2009

Synonym

Mycolicibacterium vulneris

Information on morphological and physiological properties Morphology

[Ref.: #29220]	Cell shape	rod-shaped

[Ref.: #123894]	Motility	no

[Ref.: #61691]

Incubation period

3-5 days

[Ref.: #29220]

Pigment production

yes

Multimedia content

[Ref.: #15915]

Caption:

Medium 645 37°C

[Ref.: #15915]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15915]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #15915]

Culture medium growth

[Ref.: #15915]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15915]

Culture medium composition

expand / minimize

[Ref.: #37862]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #37862]

Culture medium growth

[Ref.: #123894]

Culture medium

CIP Medium 55

[Ref.: #123894]

Culture medium growth

[Ref.: #123894]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123894]

Culture medium

CIP Medium 750

[Ref.: #123894]

Culture medium growth

[Ref.: #123894]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15915]	growth	37 °C
[Ref.: #29220]	growth	25-36 °C
[Ref.: #29220]	optimum	30.5 °C
[Ref.: #37862]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15915]	Temperature range	mesophilic
[Ref.: #29220]	Temperature range	mesophilic
[Ref.: #37862]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #61691]

Oxygen tolerance

microaerophile

[Ref.: #29220]

Ability of spore formation

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123894]		nitrate ChEBI	-	reduction
[Ref.: #123894]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123894]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #29220]	catalase	+	1.11.1.6
[Ref.: #123894]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123894]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123894]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123894]	12167	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15915]	Sample type/isolated from	human, infected wound after dogbite
[Ref.: #15915]	Geographic location (country and/or sea, region)	Limburg Province, Venlo
[Ref.: #15915]	Country	Netherlands
[Ref.: #15915]	Country ISO 3 Code	NLD
[Ref.: #15915]	Continent	Europe

[Ref.: #61691]	Sample type/isolated from	Human wound,42-yr-old woman,dog bite,leg
[Ref.: #61691]	Sampling date	2007
[Ref.: #61691]	Geographic location (country and/or sea, region)	Limburg
[Ref.: #61691]	Country	Netherlands
[Ref.: #61691]	Country ISO 3 Code	NLD
[Ref.: #61691]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human, infected wound after dogbite, a left lower leg
[Ref.: #67770]	Geographic location (country and/or sea, region)	Limburg
[Ref.: #67770]	Country	Netherlands
[Ref.: #67770]	Country ISO 3 Code	NLD
[Ref.: #67770]	Continent	Europe

[Ref.: #123894]	Sample type/isolated from	Human, Infected wound after dogbite, left lower leg
[Ref.: #123894]	Geographic location (country and/or sea, region)	Limburg
[Ref.: #123894]	Country	Netherlands
[Ref.: #123894]	Country ISO 3 Code	NLD
[Ref.: #123894]	Continent	Europe
[Ref.: #123894]	Isolation date	2007
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Human	-
#Host	#Mammals	#Canidae (Dog)
#Host Body-Site	#Other	#Wound

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT718448 (>99% sequence identity) for Mycobacterium from Microbeatlas

Aquatic Samples	4789
Soil Samples	12779
Animal Samples	3677
Plant Samples	2833
Total Samples	24078

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15915]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #123894]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15915]	Mycobacterium vulneris partial 16S rRNA gene, strain DSM 45247	LT718448	1508	ENA	547163 tax ID
[Ref.: #67770]	Mycobacterium vulneris strain NLA000700772 16S ribosomal RNA gene, complete sequence	EU834055	1471	ENA	547163 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycolicibacterium vulneris DSM 45247	GCA_000612885	contig	GenBank	547163 tax ID
[Ref.: #67770]	Mycolicibacterium vulneris DSM 45247	GCA_002104765	contig	GenBank	547163 tax ID
[Ref.: #66792]	Mycobacterium vulneris strain DSM 45247	547163.12	wgs	PATRIC	547163 tax ID	*
[Ref.: #66792]	Mycobacterium vulneris strain DSM 45247	547163.3	wgs	PATRIC	547163 tax ID	*
[Ref.: #66792]	Mycobacterium vulneris DSM 45247	2619619212	draft	JGI IMG/M	547163 tax ID	*
[Ref.: #66792]	Mycolicibacterium vulneris DSM 45247	2867085808	draft	JGI IMG/M	547163 tax ID	*

[Ref.: #67770]

GC-content

66.8 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium vulneris strain DSM 45247 PATRIC: 547163.12
[Ref.: #69480]	gram-positive	yes	87.072	no
[Ref.: #69480]	anaerobic	no	99.014	no
[Ref.: #69480]	halophile	no	93.84	no
[Ref.: #69480]	spore-forming	no	88.071	yes
[Ref.: #69480]	glucose-util	yes	68.473	no
[Ref.: #69480]	thermophile	no	97.929	yes
[Ref.: #69480]	aerobic	yes	91.495	no
[Ref.: #69480]	motile	no	93.825	no
[Ref.: #69480]	flagellated	no	98.901	no
[Ref.: #69480]	glucose-ferment	no	92.331	no

Availability in culture collections External links

[Ref.: #15915]

Culture collection no.

DSM 45247, CIP 109859, CCUG 58459, JCM 18115

[Ref.: #77965]

SI-ID 364160

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Mycobacterium vulneris DSM 45247T.	Croce O, Robert C, Raoult D, Drancourt M	Genome Announc	10.1128/genomeA.00370-14	2014	*
Phylogeny	Proposal to elevate Mycobacterium avium complex ITS sequevar MAC-Q to Mycobacterium vulneris sp. nov.	van Ingen J, Boeree MJ, Kosters K, Wieland A, Tortoli E, Dekhuijzen PN, van Soolingen D	Int J Syst Evol Microbiol	10.1099/ijs.0.008854-0	2009	*

References

#15915

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45247

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29220

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25641 (see below)

#37862 ; Curators of the CIP;
#61691 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58459

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77965

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID364160.1 )

#123894 Collection of Institut Pasteur ; Curators of the CIP; CIP 109859

#25641

IJSEM 2277 2009 ( DOI 10.1099/ijs.0.008854-0 , PubMed 19620376 )

* These data were automatically processed and therefore are not curated

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Mycobacterium vulneris strain DSM 45247

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