Name: Mycobacterium parascrofulaceum DSM 44648
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8533

Type strain:

Species: Mycobacterium parascrofulaceum

Culture col. no.: DSM 44648, ATCC BAA-614, HSC 68, JCM 13015, CIP 108112, KCTC 9979

Strain history: CIP <- 2003, C.Y. Turenne, Health Canada, Winnipeg, Manitoba, Canada: strain HSC68 <- J. Wolfe

NCBI tax ID(s): 240125 (species)

SI-ID 100401

ATCC BAA-614, DSM 44648, CIP 108112, JCM 13015, KCTC 9979

Other strains from Mycobacterium parascrofulaceum

M. parascrofulaceum M6, DSM 43222, SN 601
M. parascrofulaceum CCUG 20996
M. parascrofulaceum CCUG 27854, ATCC 35787
M. parascrofulaceum CCUG 28068
M. parascrofulaceum 10409, 144320002, CRBIP7.157, CIPT ...
M. parascrofulaceum 144320001, Harrison, CRBIP7.161, ...
M. parascrofulaceum 144320003, CRBIP7.165, CIPT 165

Section

Mycobacterium parascrofulaceum DSM 44648 is an obligate aerobe, mesophilic bacterium that was isolated from cervix biopsy.

mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium parascrofulaceum**
	Full Scientific Name (LPSN)	Mycobacterium parascrofulaceum Turenne et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #121594]	Cell shape	rod-shaped

[Ref.: #121594]	Motility	no
[Ref.: #30100]	Motility	no

[Ref.: #30100]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11957]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #11957]

Culture medium growth

[Ref.: #11957]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11957]

Culture medium composition

expand / minimize

[Ref.: #36338]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #36338]

Culture medium growth

[Ref.: #121594]

Culture medium

CIP Medium 55

[Ref.: #121594]

Culture medium growth

[Ref.: #121594]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11957]		growth	37 °C
[Ref.: #30100]		growth	25-37 °C
[Ref.: #30100]		optimum	37 °C
[Ref.: #36338]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #121594]		growth	30-37 °C
[Ref.: #121594]	no	growth	10 °C
[Ref.: #121594]	no	growth	22 °C
[Ref.: #121594]	no	growth	45 °C

[Ref.: #11957]	Temperature range	mesophilic
[Ref.: #30100]	Temperature range	mesophilic
[Ref.: #36338]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121594]	Temperature range	mesophilic
[Ref.: #121594]	Temperature range	psychrophilic
[Ref.: #121594]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121594]

Oxygen tolerance

obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30100]		NaCl		growth	5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121594]	hippurate ChEBI	-	hydrolysis
[Ref.: #121594]	nitrate ChEBI	-	reduction
[Ref.: #121594]	nitrate ChEBI	-	respiration
[Ref.: #121594]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #121594]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121594]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #30100]	catalase	+	1.11.1.6
[Ref.: #121594]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121594]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121594]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121594]	oxidase	-
[Ref.: #30100]	pyrazinamidase	+	3.5.1.B15
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #30100]	urease	+	3.5.1.5
[Ref.: #121594]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121594]	10893	-	-	+	+	-	+	+	+	+	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11957]	Sample type/isolated from	cervix biopsy
[Ref.: #11957]	Geographic location (country and/or sea, region)	Winnipeg, Manitoba
[Ref.: #11957]	Country	Canada
[Ref.: #11957]	Country ISO 3 Code	CAN
[Ref.: #11957]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Cervix biopsy
[Ref.: #67770]	Geographic location (country and/or sea, region)	Manitoba
[Ref.: #67770]	Country	Canada
[Ref.: #67770]	Country ISO 3 Code	CAN
[Ref.: #67770]	Continent	North America

[Ref.: #121594]	Sample type/isolated from	Human, Cervix biopsy
[Ref.: #121594]	Geographic location (country and/or sea, region)	Manitoba
[Ref.: #121594]	Country	Canada
[Ref.: #121594]	Country ISO 3 Code	CAN
[Ref.: #121594]	Continent	North America
[Ref.: #121594]	Isolation date	1981
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Biopsy
#Host Body-Site	#Urogenital tract	#Ovary

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11957]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121594]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #30100]	Mycobacterium parascrofulaceum strain BAA-614 16S ribosomal RNA gene, partial sequence	AY337273	1468	GenBank	525368 tax ID
[Ref.: #20218]	Mycobacterium parascrofulaceum strain BAA-614 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY337279	373	ENA	525368 tax ID	*
[Ref.: #20218]	Mycobacterium parascrofulaceum strain ATCC BAA-614 16S ribosomal RNA gene, partial sequence	GQ153273	1483	ENA	525368 tax ID	*
[Ref.: #20218]	Mycobacterium parascrofulaceum strain ATCC BAA-614 16S ribosomal RNA gene, partial sequence	JF271815	1383	ENA	525368 tax ID	*
[Ref.: #20218]	Mycobacterium parascrofulaceum strain ATCC BAA-614 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	JQ411511	441	ENA	525368 tax ID	*
[Ref.: #20218]	Mycobacterium parascrofulaceum strain ATCC BAA-614 16S-23S ribosomal RNA intergenic spacer, partial sequence	JQ411531	281	ENA	525368 tax ID	*
[Ref.: #20218]	Mycobacterium parascrofulaceum strain CIP 108112 16S ribosomal RNA gene, partial sequence	AY943211	539	ENA	240125 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacterium parascrofulaceum ATCC BAA-614	GCA_000164135	scaffold	GenBank	525368 tax ID

[Ref.: #67770]

GC-content

68.4 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium parascrofulaceum ATCC BAA-614 ATCC BAA-614 NCBI: GCA_000164135.1
[Ref.: #69480]	motile	no	93.571	yes
[Ref.: #69480]	flagellated	no	98.708	yes
[Ref.: #69480]	gram-positive	yes	89.906	no
[Ref.: #69480]	anaerobic	no	98.738	no
[Ref.: #69480]	aerobic	yes	86.484	no
[Ref.: #69480]	halophile	no	92.673	no
[Ref.: #69480]	spore-forming	no	83.709	no
[Ref.: #69480]	thermophile	no	98.436	yes
[Ref.: #69480]	glucose-util	yes	65.316	no
[Ref.: #69480]	glucose-ferment	no	91.173	no

Availability in culture collections External links

[Ref.: #11957]

Culture collection no.

DSM 44648, ATCC BAA-614, HSC 68, JCM 13015, CIP 108112, KCTC 9979

[Ref.: #77919]

SI-ID 100401

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae.	Turenne CY, Cook VJ, Burdz TV, Pauls RJ, Thibert L, Wolfe JN, Kabani A	Int J Syst Evol Microbiol	10.1099/ijs.0.02940-0	2004	*

References

#11957

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44648

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#30100

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26456 (see below)

#36338 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77919

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100401.1 )

#121594 Collection of Institut Pasteur ; Curators of the CIP; CIP 108112

#26456

IJSEM 1543 2004 ( DOI 10.1099/ijs.0.02940-0 , PubMed 15388708 )

* These data were automatically processed and therefore are not curated

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Mycobacterium parascrofulaceum ATCC BAA-614 ATCC BAA-614

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