Name: Mycobacterium heckeshornense S369
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8490

Type strain:

Species: Mycobacterium heckeshornense

Strain Designation: S369

Culture col. no.: DSM 44428, CCUG 51897, JCM 15655, CIP 107347

Strain history: CIP <- 2002, DSMZ <- A. Roth, Zehlendorf-Heckeshorn Hosp., Germany: strain S369

NCBI tax ID(s): 110505 (species)

SI-ID 50628

DSM 44428, CCUG 51897, CIP 107347, JCM 15655

Other strains from Mycobacterium heckeshornense

M. heckeshornense S504, DSM 44482
M. heckeshornense S532, DSM 44483
M. heckeshornense CCUG 52755

Section

Mycobacterium heckeshornense S369 is a microaerophile, mesophilic, rod-shaped bacterium that was isolated from respiratory tract women .

rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium heckeshornense**
	Full Scientific Name (LPSN)	Mycobacterium heckeshornense Roth et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #119854]	Cell shape	rod-shaped

[Ref.: #119854]	Motility	no

Multimedia content

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Caption:

Medium 645 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #36334]

Culture medium

MEDIUM 93 - for Mycobacterium avium subsp. sylvaticum

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Culture medium growth

[Ref.: #36334]

Culture medium composition

expand / minimize

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Culture medium

CIP Medium 93

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11548]		growth	37 °C
[Ref.: #36334]		growth	37 °C
[Ref.: #59358]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #119854]		growth	45 °C
[Ref.: #119854]	no	growth	10 °C
[Ref.: #119854]	no	growth	22 °C
[Ref.: #119854]	no	growth	30 °C

[Ref.: #11548]	Temperature range	mesophilic
[Ref.: #36334]	Temperature range	mesophilic
[Ref.: #59358]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119854]	Temperature range	thermophilic
[Ref.: #119854]	Temperature range	psychrophilic
[Ref.: #119854]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #59358]

Oxygen tolerance

microaerophile

[Ref.: #67770]

Observation

quinones: MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119854]	hippurate ChEBI	-	hydrolysis
[Ref.: #119854]	nitrate ChEBI	-	reduction
[Ref.: #119854]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119854]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119854]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119854]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119854]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #119854]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119854]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119854]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119854]	10284	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11548]	Sample type/isolated from	respiratory tract women (immunocompetent)
[Ref.: #11548]	Geographic location (country and/or sea, region)	Berlin
[Ref.: #11548]	Country	Germany
[Ref.: #11548]	Country ISO 3 Code	DEU
[Ref.: #11548]	Continent	Europe

[Ref.: #59358]	Sample type/isolated from	Human respiratory tract,woman,immunocompetent
[Ref.: #59358]	Country	Germany
[Ref.: #59358]	Country ISO 3 Code	DEU
[Ref.: #59358]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Respiratory tract of an immunocompetent women
[Ref.: #67770]	Geographic location (country and/or sea, region)	Berlin
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe

[Ref.: #119854]	Sample type/isolated from	Human, Respiratory tract
[Ref.: #119854]	Geographic location (country and/or sea, region)	Berlin
[Ref.: #119854]	Country	Germany
[Ref.: #119854]	Country ISO 3 Code	DEU
[Ref.: #119854]	Continent	Europe
[Ref.: #119854]	Isolation date	1994
* marker position based on {}

Isolation sources categories

#Host	#Human	#Female
#Host Body-Site	#Oral cavity and airways	#Airways

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF174290 (>99% sequence identity) for Mycobacterium heckeshornense from Microbeatlas

Aquatic Samples	11
Soil Samples	115
Animal Samples	2
Plant Samples	71
Total Samples	199

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11548]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119854]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11548]	Mycobacterium heckeshornense 16S ribosomal RNA gene, partial sequence	AF174290	1527	ENA	110505 tax ID
[Ref.: #20218]	Mycobacterium heckeshornense strain DSM 44428 16S ribosomal RNA gene, partial sequence	AF547934	560	ENA	110505 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium heckeshornense JCM 15655	GCA_016592155	complete	GenBank	110505 tax ID	*
[Ref.: #66792]	Mycobacterium heckeshornense strain JCM 15655	110505.9	complete	PATRIC	110505 tax ID	*
[Ref.: #66792]	Mycobacterium heckeshornense DSM 44428	GCA_025822025	scaffold	GenBank	110505 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium heckeshornense JCM 15655 NCBI: GCA_016592155.1
[Ref.: #69480]	flagellated	no	99.274	no
[Ref.: #69480]	gram-positive	yes	86.935	no
[Ref.: #69480]	anaerobic	no	98.32	no
[Ref.: #69480]	aerobic	yes	89.838	no
[Ref.: #69480]	halophile	no	93.802	no
[Ref.: #69480]	spore-forming	no	84.976	no
[Ref.: #69480]	thermophile	no	86.942	no
[Ref.: #69480]	glucose-util	yes	68.923	no
[Ref.: #69480]	motile	no	96.774	no
[Ref.: #69480]	glucose-ferment	no	92.377	no

Availability in culture collections External links

[Ref.: #11548]

Culture collection no.

DSM 44428, CCUG 51897, JCM 15655, CIP 107347

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SI-ID 50628

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Mycobacterium heckeshornense JCM 15655(T), Closely Related to a Pathogenic Nontuberculous Mycobacterial Species, Mycobacterium xenopi.	Yoshida M, Fukano H, Asakura T, Suzuki M, Hoshino Y	Microbiol Resour Announc	10.1128/MRA.00020-21	2021	*
Phylogeny	Mycobacterium heckeshornense sp. nov., A new pathogenic slowly growing Mycobacterium sp. Causing cavitary lung disease in an immunocompetent patient.	Roth A, Reischl U, Schonfeld N, Naumann L, Emler S, Fischer M, Mauch H, Loddenkemper R, Kroppenstedt RM	J Clin Microbiol	10.1128/JCM.38.11.4102-4107.2000	2000	*

References

#11548

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44428

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36334 ; Curators of the CIP;
#59358 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51897

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77884

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID50628.1 )

#119854 Collection of Institut Pasteur ; Curators of the CIP; CIP 107347
* These data were automatically processed and therefore are not curated

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Mycobacterium heckeshornense JCM 15655

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