Mycobacterium heidelbergense (DSM 44471, ATCC 51253, CIP 105424)

Name: Mycobacterium heidelbergense (DSM 44471, ATCC 51253, CIP 105424)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8485

Type strain

Strain Designation 2554/91

Culture col. no. DSM 44471 ATCC 51253 CIP 105424 2554/91 JCM 14842

NCBI tax ID(s) 53376

Special Collections DSMZ JCM CIP

Links

Mycobacterium heidelbergense 2554/91 is a facultative anaerobe, mesophilic, rod-shaped prokaryote that was isolated from child .

rod-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 42879

ATCC 51253,CIP 105424,DSM 44471,JCM 14842

@ref 20215

Last LPSN update 2025-12-16 09:48:51

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium heidelbergense

Full scientific name Mycobacterium heidelbergense Haas et al. 1998

BacDive ID	Other strains from **Mycobacterium heidelbergense** (3)	Type strain
8484	M. heidelbergense B99, DSM 44554
150816	M. heidelbergense CCUG 42424 A
150817	M. heidelbergense CCUG 42424 B

Morphology

Cell morphology

@ref	Cell shape	Gram stain	Confidence
119856	rod-shaped
125439		positive	94.6
125438			90.5

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11797	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11797	LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)	Medium recipe at MediaDive	Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
36315	MEDIUM 55 - for Mycobacterium
119856	CIP Medium 55	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11797	positive	growth	37	mesophilic
36315	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
119856	positive	growth	30-37	mesophilic
119856	negative	growth	10
119856	negative	growth	22
119856	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
119856	facultative anaerobe
125439	obligate aerobe	96.2

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119856	606565 ChEBI	hippurate	-	hydrolysis
119856	17632 ChEBI	nitrate	-	reduction
119856	17632 ChEBI	nitrate	+	respiration
119856	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119856	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
119856	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119856	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119856	gamma-glutamyltransferase	-	2.3.2.2
119856	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119856	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
119856	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119856	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	-
#Host Body-Site	#Organ	#Lymph node
#Host	#Human	#Child

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11797	child (lymph node)		Germany	DEU	Europe
67770	Cervical lymph node	Heidelberg	Germany	DEU	Europe
119856	Human, Lymph node (child)		Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11797	2	Risk group (German classification) According to TRBA 466
119856	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1073074v1 assembly for Mycobacterium heidelbergense JCM 14842	complete	GCA_010730745	53376.9	8112685497	53376	98.02
67770	ASM208621v1 assembly for Mycobacterium heidelbergense DSM 44471	contig	GCA_002086215	53376.3	2865543863	53376	56.96
66792	ASM2582194v1 assembly for Mycobacterium heidelbergense DSM 44471	scaffold	GCA_025821945	53376.11	8114819467	53376	48.76

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Mycobacterium heidelbergense 16S rRNA gene	AJ000684	1445	53376
20218	M.heidelbergense 16S rRNA	X70960	1446	53376
20218	Mycobacterium heidelbergense strain CIP 105424 16S ribosomal RNA gene, partial sequence	AF547935	542	53376
20218	Mycobacterium heidelbergense strain DSM 44471 16S-23S ribosomal RNA intergenic spacer, partial sequence	HM022203	283	53376

GC content

@ref	GC-content (mol%)	Method
67770	68	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mycobacterium heidelbergense strain JCM 14842
PATRIC: 53376.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	52.40	no
125439	motility	BacteriaNetⓘ	no	82.10	no
125439	gram_stain	BacteriaNetⓘ	positive	94.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycobacterium heidelbergense JCM 14842
NCBI: GCA_010730745.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.88	no
125438	anaerobic	anaerobicⓘ	no	94.32	yes
125438	aerobic	aerobicⓘ	yes	65.16	yes
125438	spore-forming	spore-formingⓘ	no	69.20	no
125438	thermophilic	thermophileⓘ	no	91.71	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Mycobacterium terramassiliense, Mycobacterium rhizamassiliense and Mycobacterium numidiamassiliense sp. nov., three new Mycobacterium simiae complex species cultured from plant roots.	Bouam A, Armstrong N, Levasseur A, Drancourt M.	Sci Rep	10.1038/s41598-018-27629-1	2018
Phylogeny	A new agent of mycobacterial lymphadenitis in children: Mycobacterium heidelbergense sp. nov.	Haas WH, Butler WR, Kirschner P, Plikaytis BB, Coyle MB, Amthor B, Steigerwalt AG, Brenner DJ, Salfinger M, Crawford JT, Bottger EC, Bremer HJ	J Clin Microbiol	10.1128/jcm.35.12.3203-3209.1997	1997

References

#11797	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44471
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36315	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#119856	Collection of Institut Pasteur ; Curators of the CIP; CIP 105424
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium heidelbergense (DSM 44471, ATCC 51253, CIP 105424)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_010730745

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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