Mycobacterium septicum Australia 9330 is an aerobe, rod-shaped bacterium that was isolated from catheter tip of centrally located Hickman catheter.
rod-shaped aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Mycobacteriaceae |
| Genus Mycobacterium |
| Species Mycobacterium septicum |
| Full scientific name Mycobacterium septicum Schinsky et al. 2000 |
| Synonyms (1) |
| BacDive ID | Other strains from Mycobacterium septicum (3) | Type strain |
|---|---|---|
| 8249 | M. septicum DSM 43076, IMRU 12 | |
| 8257 | M. septicum Gent 5, DSM 43480, IMET 10653, SN 252 Typ ... | |
| 136951 | M. septicum D13, CIP 108512 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11717 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | Medium recipe at MediaDive | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water | ||
| 19860 | MB7H10 | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |||
| 19860 | MB7H11 | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |||
| 19860 | 5006 | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |||
| 36370 | MEDIUM 55 - for Mycobacterium | ||||
| 122566 | CIP Medium 55 | Medium recipe at CIP |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 122566 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 122566 | 17632 ChEBI | nitrate | + | reduction | |
| 122566 | 17632 ChEBI | nitrate | - | respiration | |
| 122566 | 16301 ChEBI | nitrite | - | reduction | |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68379 | alkaline phosphatase | - | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | - | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | - | 3.2.1.20 | from API Coryne |
| 122566 | amylase | - | ||
| 122566 | beta-galactosidase | - | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 122566 | caseinase | - | 3.4.21.50 | |
| 122566 | catalase | + | 1.11.1.6 | |
| 122566 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 122566 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 122566 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 122566 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 122566 | lipase | - | ||
| 68382 | lipase (C 14) | + | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 122566 | oxidase | - | ||
| 122566 | protease | - | ||
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 122566 | tween esterase | + | ||
| 122566 | urease | - | 3.5.1.5 |
| Metadata FA analysis | ||||||||||||||||||||||||||||||||||||||||||||||
| type of FA analysis | whole cell analysis | |||||||||||||||||||||||||||||||||||||||||||||
| method/protocol | CCUG | |||||||||||||||||||||||||||||||||||||||||||||
| @ref | 55946 | |||||||||||||||||||||||||||||||||||||||||||||
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| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Infection | #Medical device | - | |
| #Infection | #Medical device | #Catheter |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | Latitude | Longitude | Isolation date | |
|---|---|---|---|---|---|---|---|---|---|
| 11717 | catheter tip of centrally located Hickman catheter | Melbourne | Australia | AUS | Australia and Oceania | -37.8082 | 144.958 -37.8082/144.958 | ||
| 55946 | Catheter tip of centrally located Hickman catheter | Melbourne | Australia | AUS | Australia and Oceania | ||||
| 67770 | Catheter tip of centrally located Hickman catheter | Melbourne | Australia | AUS | Australia and Oceania | ||||
| 122566 | Catheter tip of centrally located Hickman | Melbourne | Australia | AUS | Australia and Oceania | 1990 |
Global distribution of 16S sequence NR_042916 (>99% sequence identity) for Mycolicibacterium from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM1239642v1 assembly for Mycolicibacterium septicum DSM 44393 ATCC 700731 | contig | 1341646 | 72.4 | ||||
| 67770 | Mycobacterium_septicum_DSM44393T assembly for Mycolicibacterium septicum DSM 44393 type strain: DSM 44393 | scaffold | 1341646 | 45.12 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Mycolicibacterium septicum strain DSM 44393 16S ribosomal RNA gene, partial sequence | AY457070 | 1483 | 98668 | ||
| 20218 | Mycobacterium septicum strain ATCC 700731 16S ribosomal RNA gene, partial sequence | JQ899228 | 642 | 1341646 | ||
| 20218 | Mycobacterium septicum strain ATCC 700731 16S ribosomal RNA gene, partial sequence | JQ899250 | 643 | 1341646 | ||
| 20218 | Mycobacterium septicum strain CIP 106642 16S ribosomal RNA gene, partial sequence | AF547964 | 540 | 1341646 | ||
| 20218 | Mycobacterium septicum 16S ribosomal RNA gene, partial sequence | AF111809 | 1491 | 1341646 | ||
| 11717 | Mycolicibacterium septicum DSM 44393 16S ribosomal RNA, partial sequence | NR_042916 | 1483 | 1341646 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | no | 61.10 | no |
| 125439 | motility | BacteriaNetⓘ | no | 60.40 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 90.20 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 96.00 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 92.22 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 97.59 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 61.96 | no |
| 125438 | aerobic | aerobicⓘ | yes | 87.78 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 98.00 | no |
| 125438 | flagellated | motile2+ⓘ | no | 91.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Draft Genome Sequence of Mycobacterium neworleansense Strain ATCC 49404T. | Asmar S, Robert C, Croce O, Caputo A, Drancourt M. | Genome Announc | 10.1128/genomea.01314-15 | 2015 | ||
| Draft Genome Sequence of Mycobacterium peregrinum Strain CSUR P2098. | Asmar S, Rascovan N, Robert C, Drancourt M. | Genome Announc | 10.1128/genomea.01274-15 | 2015 | ||
| Phylogeny | Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns. | Fogelson SB, Camus AC, Lorenz WW, Vasireddy R, Vasireddy S, Smith T, Brown-Elliott BA, Wallace RJ, Hasan NA, Reischl U, Sanchez S. | PLoS One | 10.1371/journal.pone.0214274 | 2019 | |
| Phylogeny | Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera. | Gupta RS, Lo B, Son J. | Front Microbiol | 10.3389/fmicb.2018.00067 | 2018 | |
| In Vitro Activity of the Sudapyridine (WX-081) against Non-Tuberculous Mycobacteria Isolated in Beijing, China. | Zhu R, Shang Y, Chen S, Xiao H, Ren R, Wang F, Xue Y, Li L, Li Y, Chu N, Huang H. | Microbiol Spectr | 10.1128/spectrum.01372-22 | 2022 | ||
| In Vitro Antimicrobial Activities of Tigecycline, Eravacycline, Omadacycline, and Sarecycline against Rapidly Growing Mycobacteria. | Zhang T, Du J, Dong L, Wang F, Zhao L, Jia J, Wang C, Cheng M, Yu X, Huang H. | Microbiol Spectr | 10.1128/spectrum.03238-22 | 2023 | ||
| Pathogenicity | In vitro activities of tigecycline and 10 other antimicrobials against nonpigmented rapidly growing mycobacteria. | Fernandez-Roblas R, Martin-de-Hijas NZ, Fernandez-Martinez AI, Garcia-Almeida D, Gadea I, Esteban J. | Antimicrob Agents Chemother | 10.1128/aac.00695-08 | 2008 | |
| Pathogenicity | In Vitro Activities of Bedaquiline and Delamanid against Nontuberculous Mycobacteria Isolated in Beijing, China. | Yu X, Gao X, Li C, Luo J, Wen S, Zhang T, Ma Y, Dong L, Wang F, Huang H. | Antimicrob Agents Chemother | 10.1128/aac.00031-19 | 2019 | |
| Mycobacterial biomaterials and resources for researchers. | Hazbon MH, Rigouts L, Schito M, Ezewudo M, Kudo T, Itoh T, Ohkuma M, Kiss K, Wu L, Ma J, Hamada M, Strong M, Salfinger M, Daley CL, Nick JA, Lee JS, Rastogi N, Couvin D, Hurtado-Ortiz R, Bizet C, Suresh A, Rodwell T, Albertini A, Lacourciere KA, Deheer-Graham A, Alexander S, Russell JE, Bradford R, Riojas MA. | Pathog Dis | 10.1093/femspd/fty042 | 2018 | ||
| Phylogeny | Rapid identification of mycobacterial whole cells in solid and liquid culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | Lotz A, Ferroni A, Beretti JL, Dauphin B, Carbonnelle E, Guet-Revillet H, Veziris N, Heym B, Jarlier V, Gaillard JL, Pierre-Audigier C, Frapy E, Berche P, Nassif X, Bille E. | J Clin Microbiol | 10.1128/jcm.01397-10 | 2010 | |
| Genetics | Noncontiguous Genome Sequence of Mycobacterium septicum Strain DSM 44393T. | Sassi M, Robert C, Raoult D, Drancourt M | Genome Announc | 10.1128/genomeA.00574-13 | 2013 | |
| Phylogeny | Isolation of Mycobacterium septicum from the sputum of a patient suffering from hemoptoic pneumonia. | Adekambi T, Drancourt M | Res Microbiol | 10.1016/j.resmic.2005.10.006 | 2005 | |
| Phylogeny | Mycobacterium septicum sp. nov., a new rapidly growing species associated with catheter-related bacteraemia. | Schinsky MF, McNeil MM, Whitney AM, Steigerwalt AG, Lasker BA, Floyd MM, Hogg GG, Brenner DJ, Brown JM | Int J Syst Evol Microbiol | 10.1099/00207713-50-2-575 | 2000 |
| #11717 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44393 |
| #19860 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #36370 | ; Curators of the CIP; |
| #55946 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43574 |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #122566 | Collection of Institut Pasteur ; Curators of the CIP; CIP 106642 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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