Mycobacterium murale 220-96 is an aerobe, rod-shaped bacterium that was isolated from water-damaged indoor building material.
rod-shaped aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Mycobacteriaceae |
| Genus Mycobacterium |
| Species Mycobacterium murale |
| Full scientific name Mycobacterium murale Vuorio et al. 1999 |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11686 | MYCOBACTERIUM MEDIUM (DSMZ Medium 219) | Medium recipe at MediaDive | Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water | ||
| 11686 | PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) | Medium recipe at MediaDive | Name: PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) Composition: Agar 20.0 g/l Proteose peptone no. 3 5.0 g/l Meat extract 3.0 g/l Glycerol Distilled water | ||
| 20046 | MB7H10 | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |||
| 20046 | MB7H11 | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |||
| 20046 | 5006 | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |||
| 36255 | MEDIUM 55 - for Mycobacterium | ||||
| 121186 | CIP Medium 55 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-9(H2) (90%), MK-8(H2) (10%) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68369 | 29016 ChEBI | arginine | - | hydrolysis | from API 20NE |
| 68379 | 17634 ChEBI | D-glucose | + | fermentation | from API Coryne |
| 68369 | 17634 ChEBI | D-glucose | - | fermentation | from API 20NE |
| 68379 | 16899 ChEBI | D-mannitol | + | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 68369 | 4853 ChEBI | esculin | - | hydrolysis | from API 20NE |
| 68379 | 5291 ChEBI | gelatin | + | hydrolysis | from API Coryne |
| 68369 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20NE |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 121186 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | + | fermentation | from API Coryne |
| 68369 | 25115 ChEBI | malate | - | assimilation | from API 20NE |
| 68379 | 17306 ChEBI | maltose | + | fermentation | from API Coryne |
| 68369 | 17306 ChEBI | maltose | - | assimilation | from API 20NE |
| 68379 | 17632 ChEBI | nitrate | + | reduction | from API Coryne |
| 121186 | 17632 ChEBI | nitrate | - | reduction | |
| 121186 | 17632 ChEBI | nitrate | - | respiration | |
| 68369 | 17632 ChEBI | nitrate | - | reduction | from API 20NE |
| 121186 | 16301 ChEBI | nitrite | - | reduction | |
| 68379 | 17992 ChEBI | sucrose | + | fermentation | from API Coryne |
| 68369 | 27897 ChEBI | tryptophan | - | energy source | from API 20NE |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| 68369 | 16199 ChEBI | urea | - | hydrolysis | from API 20NE |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68379 | alkaline phosphatase | - | 3.1.3.1 | from API Coryne |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68379 | alpha-glucosidase | - | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 121186 | amylase | - | ||
| 68369 | arginine dihydrolase | - | 3.5.3.6 | from API 20NE |
| 121186 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | + | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68369 | beta-glucosidase | - | 3.2.1.21 | from API 20NE |
| 68379 | beta-glucuronidase | + | 3.2.1.31 | from API Coryne |
| 121186 | caseinase | - | 3.4.21.50 | |
| 121186 | catalase | + | 1.11.1.6 | |
| 121186 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 121186 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 121186 | gelatinase | - | ||
| 68369 | gelatinase | - | from API 20NE | |
| 68379 | gelatinase | + | from API Coryne | |
| 121186 | lecithinase | - | ||
| 121186 | lipase | - | ||
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 121186 | oxidase | - | ||
| 121186 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 121186 | tween esterase | + | ||
| 121186 | urease | + | 3.5.1.5 | |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
| 68369 | urease | - | 3.5.1.5 | from API 20NE |
| Metadata FA analysis | ||||||||||||||||||||||||||||||||||||||||
| type of FA analysis | whole cell analysis | |||||||||||||||||||||||||||||||||||||||
| method/protocol | CCUG | |||||||||||||||||||||||||||||||||||||||
| @ref | 54907 | |||||||||||||||||||||||||||||||||||||||
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| @ref | Control | Alkaline phosphatase | Esterase (C 4) | 2-naphtyl caprylateEsterase Lipase (C 8) | Lipase (C 14) | L-leucyl-2-naphthylamideLeucine arylamidase | L-valyl-2-naphthylamideValine arylamidase | L-cystyl-2-naphthylamideCystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20046 | not determinedn.d. | - | + | + | - | - | - | - | - | - | - | - | - | + | + | - | + | - | - | - | |
| 121186 | - | + | + | + | - | + | + | + | + | - | + | + | - | - | - | + | + | - | - | - | |
| 11686 | - | + | + | + | +/- | + | + | + | +/- | +/- | + | + | - | - | - | + | + | - | - | - |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|---|
| 11686 | water-damaged indoor building material | Finland | FIN | Europe | ||
| 54907 | Water-damaged indoor building material | Finland | FIN | Europe | ||
| 67770 | Water-damaged indoor building material of a children's day care center | Finland | FIN | Europe | ||
| 121186 | Environment, Water-damaged indoor building material | Helsinki | Finland | FIN | Europe |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM1072299v1 assembly for Mycolicibacterium murale JCM 13392 | contig | 182220 | 72.71 | ||||
| 66792 | ASM2582230v1 assembly for Mycolicibacterium murale DSM 44340 | scaffold | 182220 | 59.04 | ||||
| 124043 | ASM3952889v1 assembly for Mycolicibacterium murale JCM 13392 | scaffold | 182220 | 35.13 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Mycobacterium murale 16S ribosomal RNA gene, partial sequence | HM770863 | 1472 | 182220 | ||
| 20218 | Mycobacterium murale strain CIP 105980 16S ribosomal RNA gene, partial sequence | AF547950 | 540 | 182220 | ||
| 20218 | Mycobacterium murale gene for 16S ribosomal RNA, partial sequence | AB537171 | 1459 | 182220 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 98.28 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 91.50 | no |
| 125439 | motility | BacteriaNetⓘ | no | 89.34 | no |
| 125439 | spore_formation | BacteriaNetⓘ | no | 73.85 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 90.34 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 97.51 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 63.36 | no |
| 125438 | aerobic | aerobicⓘ | yes | 91.39 | no |
| 125438 | thermophilic | thermophileⓘ | no | 96.17 | no |
| 125438 | flagellated | motile2+ⓘ | no | 87.46 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Phylogeny | Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera. | Gupta RS, Lo B, Son J. | Front Microbiol | 10.3389/fmicb.2018.00067 | 2018 | |
| Phylogeny | Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species. | Cheng Y, Lei W, Wang X, Tian Z, Liu H, Yang J, Lu S, Lai XH, Pu J, Huang Y, Zhang S, Yang C, Lian X, Bai Y, Wan K, Wang S, Xu J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005019 | 2021 | |
| Phylogeny | A new rapidly growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre. | Vuorio R, Andersson MA, Rainey FA, Kroppenstedt RM, Kampfer P, Busse HJ, Viljanen M, Salkinoja-Salonen M | Int J Syst Bacteriol | 10.1099/00207713-49-1-25 | 1999 |
| #11686 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44340 |
| #20046 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #36255 | ; Curators of the CIP; |
| #54907 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 39728 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68369 | Automatically annotated from API 20NE . |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #121186 | Collection of Institut Pasteur ; Curators of the CIP; CIP 105980 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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