Mycobacterium bohemicum 2938-X86 | Type strain | CCUG 39919, CIP 105811, DSM 44277

Strain identifier

BacDive ID: 8474

Type strain:

Species: Mycobacterium bohemicum

Strain Designation: 2938-X86

Culture col. no.: CCUG 39919, CIP 105811, DSM 44277

Strain history: 1998, U. Reischl and L. Naumann, Inst. für Med., Regensburg, Germany

Section

Name and taxonomic classification
Morphology
Culture and growth conditions
Physiology and metabolism
Isolation, sampling and environmental information
Safety information
Sequence information

External links

Mycobacterium bohemicum 2938-X86 is a mesophilic human pathogen that was isolated from sputum.

human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Actinobacteria
	Class	Actinobacteria
	Order	Mycobacteriales
	Family	Mycobacteriaceae
	Genus	Mycobacterium
	Species	Mycobacterium bohemicum
	Full Scientific Name (PNU)	Mycobacterium bohemicum Reischl et al. 1998

Information on morphological and physiological propertiesMorphology

[Ref.: #20039]	Colony color	Beige (1001)
[Ref.: #20039]	Incubation period	10-14 days
[Ref.: #20039]	Cultivation medium used	5006

[Ref.: #20039]	Colony color	Beige (1001)
[Ref.: #20039]	Incubation period	10-14 days
[Ref.: #20039]	Cultivation medium used	BHI

[Ref.: #20039]	Colony color	Beige (1001)
[Ref.: #20039]	Incubation period	10-14 days
[Ref.: #20039]	Cultivation medium used	BUG

[Ref.: #20039]	Colony color	Corn yellow (1006)
[Ref.: #20039]	Incubation period	10-14 days
[Ref.: #20039]	Cultivation medium used	MB7H9

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #11643]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #11643]

Culture medium growth

[Ref.: #11643]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #20039]

Culture medium

BUG

[Ref.: #20039]

Culture medium growth

[Ref.: #20039]

Culture medium

BHI

[Ref.: #20039]

Culture medium growth

[Ref.: #20039]

Culture medium

5006

[Ref.: #20039]

Culture medium growth

[Ref.: #20039]

Culture medium composition

expand / minimize

[Ref.: #20039]

Culture medium

MB7H9

[Ref.: #20039]

Culture medium growth

[Ref.: #20039]

Culture medium composition

expand / minimize

[Ref.: #36252]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #36252]

Culture medium growth

		Temperature
[Ref.: #11643]	growth	37 ̊C
[Ref.: #20039]	optimum	37 ̊C
[Ref.: #36252]	growth	37 ̊C
[Ref.: #54992]	growth	37 ̊C

[Ref.: #11643]	Temperature range	mesophilic
[Ref.: #20039]	Temperature range	mesophilic
[Ref.: #36252]	Temperature range	mesophilic
[Ref.: #54992]	Temperature range	mesophilic

Information on physiology and metabolismPhysiology and metabolism

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20039]	398	-	+	-	-	-	-	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	+/-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20039]	824	not determinedn.d.	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #11643]	5224	-	-	+/-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #11643]	15053	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #11643]	Sample type/isolated from	sputum
[Ref.: #11643]	Geographic location (country and/or sea, region)	Ostrava
[Ref.: #11643]	Country	Czech Republic
[Ref.: #11643]	Country ISO 3 Code	CZE
[Ref.: #11643]	Continent	Europe

[Ref.: #54992]	Sample type/isolated from	Human sputum,53-yr-old patient with Down's syndr.
[Ref.: #54992]	Sampling date	1991
[Ref.: #54992]	Country	Czech Republic
[Ref.: #54992]	Country ISO 3 Code	CZE
[Ref.: #54992]	Continent	Europe

Isolation sources categories

#Host Body Product

#Fluids

#Sputum

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsSafety information

[Ref.: #11643]	Pathogenicity (human)	yes
[Ref.: #11643]	Pathogenicity (animal)	yes
[Ref.: #11643]	Biosafety level	1	Risk group (German classification)
[Ref.: #20039]	Biosafety level	1	German classification

Information on genomic background e.g. entries in nucleic sequence databassSequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Mycobacterium bohemicum strain CIP 105811 16S ribosomal RNA gene, partial sequence	AF547901	554	ENA	56425 tax ID	*
[Ref.: #20218]	Mycobacterium bohemicum 16S ribosomal RNA gene, partial sequence	U84502	1516	ENA	56425 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium bohemicum DSM 44277	GCA_001053185	scaffold	GenBank	1236609 tax ID
[Ref.: #66792]	Mycobacterium bohemicum DSM 44277	GCA_002102025	contig	GenBank	56425 tax ID
[Ref.: #66792]	Mycobacterium bohemicum DSM 44277	1236609.3	wgs	PATRIC	1236609 tax ID
[Ref.: #66792]	Mycobacterium bohemicum strain DSM 44277	56425.4	wgs	PATRIC	56425 tax ID

Availability in culture collectionsExternal links

[Ref.: #11643]

Culture collection no.

CCUG 39919, CIP 105811, DSM 44277

[Ref.: #20218]

Associated Passport(s) in StrainInfo

169403

References

#11643

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44277

#20039

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36252 ; Curators of the CIP;
#54992 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 39919

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These References are textmined

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