Mycobacterium pulveris (CCUG 37668, CIP 106804, KCTC 9518)

Name: Mycobacterium pulveris (CCUG 37668, CIP 106804, KCTC 9518)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8468

Type strain

Strain Designation 33505

Culture col. no. CCUG 37668 CIP 106804 KCTC 9518 DSM 44222 ATCC 35154 4 more

NCBI tax ID(s) 36813

Special Collections DSMZ CCUG JCM KCTC CIP Wink compendium

History <- A. F. Yassin <- ATCC <- M. Tsukamura, NCH 33505 <- M Tsukamura M. Tsukamura 33505. CIP <- 2000, JCM <- M. Tsukamura: strain 33505

Links

Mycobacterium pulveris 33505 is an aerobe, Gram-positive bacterium that was isolated from house dust.

Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40892

CT2025-00075,ATCC 35154,CCUG 37668,JCM 6370,CIP 106804,DSM 44222,CIP 1480001,KCTC 9518,CNCTC 7289

@ref 20215

Last LPSN update 2026-02-09 11:19:42

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium pulveris

Full scientific name Mycobacterium pulveris Tsukamura et al. 1983

Synonyms (1)

Mycolicibacterium pulveris

Morphology

Cell morphology

@ref	Cell shape	Gram stain	Confidence
67771		positive
122052	rod-shaped
125439		positive	97.2
125438			92
125438		positive	90.154

Colony morphology

@ref	Colony color	Incubation period	Medium used
19852	Zinc yellow	10-14 days	5006
19852	Zinc yellow	10-14 days	MB7H10
19852	Zinc yellow	10-14 days	MB7H11
53795		3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11606	LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)	Medium recipe at MediaDive	Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
11606	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
19852	MB7H10		Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
19852	MB7H11		Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
19852	5006		Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36380	MEDIUM 55 - for Mycobacterium
122052	CIP Medium 55	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11606	positive	growth	37
19852	positive	optimum	37
36380	positive	growth	37
53795	positive	growth	37
67770	positive	growth	37
67771	positive	growth	37
122052	positive	growth	22-45
122052	negative	growth	10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
53795	aerobe
67771	aerobe
122052	obligate aerobe
125439	obligate aerobe	99.2

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
122052	606565 ChEBI	hippurate	-	hydrolysis
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
122052	17632 ChEBI	nitrate	+	reduction
122052	17632 ChEBI	nitrate	-	respiration
122052	16301 ChEBI	nitrite	-	reduction
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122052	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
122052	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
122052	catalase	+	1.11.1.6
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122052	gamma-glutamyltransferase	-	2.3.2.2
122052	gelatinase	-
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122052	oxidase	-
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
122052	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19852	-	+	+	+/-	-	-	+	-	-	+/-	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19852	not determinedn.d.	+	+	+	+	+	-	+	-	-	+	+	-	-	-	+	-	-	-	-
122052	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#House
#Engineered	#Waste	#Dust (Ash)

Isolation

@ref	Sample type
11606	house dust
53795	House dust
67770	House dust
67771	From house dust
122052	Environment, House dust

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11606	1	Risk group (German classification) According to TRBA 466
19852	1	Risk group (German classification) According to TRBA 466
122052	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1072572v1 assembly for Mycolicibacterium pulveris JCM 6370	complete	GCA_010725725	36813.7	2996216305	36813	98.62
66792	ASM2582210v1 assembly for Mycolicibacterium pulveris DSM 44222	scaffold	GCA_025822105	36813.9	8115232036	36813	59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11606	Mycobacterium pulveris 16S rRNA gene, strain DSM 44222T	AJ429046	1492		36813
124043	Mycobacterium pulveris strain CIP 106804 16S ribosomal RNA gene, partial sequence.	AF547960	546		36813

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mycolicibacterium pulveris strain JCM 6370
PATRIC: 36813.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	58.10	no
125439	motility	BacteriaNetⓘ	no	81.10	no
125439	gram_stain	BacteriaNetⓘ	positive	97.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycolicibacterium pulveris JCM 6370
NCBI: GCA_010725725.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.15	yes
125438	anaerobic	anaerobicⓘ	no	96.10	yes
125438	aerobic	aerobicⓘ	yes	83.39	yes
125438	spore-forming	spore-formingⓘ	no	60.10	no
125438	thermophilic	thermophileⓘ	no	94.40	yes
125438	flagellated	motile2+ⓘ	no	92.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.	Gupta RS, Lo B, Son J.	Front Microbiol	10.3389/fmicb.2018.00067	2018
	Genomics Insights into Mycolicibacterium Hassiacum Causing Infection in a Cat with Pyogranulomatous Dermatitis and Panniculitis.	Smedile D, Iurescia M, Carfora V, Cocumelli C, Palmerini T, Diaconu EL, Congiu I, Donati V, Stravino F, Sorbara L, Romano E, Caprioli A, Battisti A.	Pathogens	10.3390/pathogens13090785	2024
	In Vitro Activity of the Sudapyridine (WX-081) against Non-Tuberculous Mycobacteria Isolated in Beijing, China.	Zhu R, Shang Y, Chen S, Xiao H, Ren R, Wang F, Xue Y, Li L, Li Y, Chu N, Huang H.	Microbiol Spectr	10.1128/spectrum.01372-22	2022
	In Vitro Antimicrobial Activities of Tigecycline, Eravacycline, Omadacycline, and Sarecycline against Rapidly Growing Mycobacteria.	Zhang T, Du J, Dong L, Wang F, Zhao L, Jia J, Wang C, Cheng M, Yu X, Huang H.	Microbiol Spectr	10.1128/spectrum.03238-22	2023
	In vitro and intracellular inhibitory activities of nosiheptide against Mycobacterium abscessus.	Zhu R, Yu X, Zhang T, Kong Y, Wang F, Jia J, Xue Y, Huang H.	Front Microbiol	10.3389/fmicb.2022.926361	2022
Pathogenicity	In Vitro Activities of Bedaquiline and Delamanid against Nontuberculous Mycobacteria Isolated in Beijing, China.	Yu X, Gao X, Li C, Luo J, Wen S, Zhang T, Ma Y, Dong L, Wang F, Huang H.	Antimicrob Agents Chemother	10.1128/aac.00031-19	2019
Pathogenicity	Dual-probe assay for rapid detection of drug-resistant Mycobacterium tuberculosis by real-time PCR.	Wada T, Maeda S, Tamaru A, Imai S, Hase A, Kobayashi K.	J Clin Microbiol	10.1128/jcm.42.11.5277-5285.2004	2004
	Comparative Analysis of Virulence Genes in Non-Tuberculosis Mycobacteria (NTM) Isolated from Kenyan Camel Milk Suggests Potential Pathogenicity.	Wanjiru ML, Matofari JW, Bebe BO, Njeru MJ, Nduko JM.	Curr Microbiol	10.1007/s00284-025-04244-8	2025
	Lymphocutaneous spread of Mycobacterium elephantis in an immunocompetent individual: A case report.	Edwards BD, Somayaji R, Fisher D, Chia JC.	SAGE Open Med Case Rep	10.1177/2050313x211034913	2021
Enzymology	Isolation of mycobacteria from clinical samples collected in the United States from 2004 to 2011.	Thacker TC, Robbe-Austerman S, Harris B, Van Palmer M, Waters WR.	BMC Vet Res	10.1186/1746-6148-9-100	2013
Phylogeny	Numerical classification of rapidly growing nonphotochromogenic mycobacteria.	Tsukamura M, Ichiyama S.	Microbiol Immunol	10.1111/j.1348-0421.1986.tb03014.x	1986
	Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.	Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methe B, DeSantis TZ, Human Microbiome Consortium, Petrosino JF, Knight R, Birren BW.	Genome Res	10.1101/gr.112730.110	2011
Genetics	Characterization of Mycobacterium salfingeri sp. nov.: A novel nontuberculous mycobacteria isolated from a human wound infection.	Musser E, Smith C, Halse TA, Kohlerschmidt D, Rourke A, Fiero A, Musser KA, Escuyer V, Lapierre P.	Front Microbiol	10.3389/fmicb.2022.992610	2022

References

#11606	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44222
#19852	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#36380	; Curators of the CIP;
#53795	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37668
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#122052	Collection of Institut Pasteur ; Curators of the CIP; CIP 106804
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium pulveris (CCUG 37668, CIP 106804, KCTC 9518)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_010725725

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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